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AB324352

Anti-PYCR2 antibody [EPR28825-54]

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Rabbit Recombinant Monoclonal PYCR2 antibody. Suitable for WB and reacts with Human samples.

View Alternative Names

Pyrroline-5-carboxylate reductase 2, P5C reductase 2, P5CR 2, PYCR2

3 Images
Western blot - Anti-PYCR2 antibody [EPR28825-54] (AB324352)
  • WB

Supplier Data

Western blot - Anti-PYCR2 antibody [EPR28825-54] (AB324352)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : Lane 1 : 180 seconds; Lanes 2-3 : 81 seconds.

All lanes:

Western blot - Anti-PYCR2 antibody [EPR28825-54] (ab324352) at 1/1000 dilution

Lane 1:

A549 (human lung carcinoma epithelial cell) whole cell lysate at 30 µg

Lane 2:

HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate at 30 µg

Lane 3:

SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 30 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 34 kDa

false

Western blot - Anti-PYCR2 antibody [EPR28825-54] (AB324352)
  • WB

Supplier Data

Western blot - Anti-PYCR2 antibody [EPR28825-54] (AB324352)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lanes 1-2 : 180 seconds; Lane 3 : 37 seconds.

All lanes:

Western blot - Anti-PYCR2 antibody [EPR28825-54] (ab324352) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 30 µg

Lane 2:

HeLa transfected with siRNA specifically targeting PYCR2 whole cell lysate at 30 µg

Lane 3:

Jurkat (human t cell leukemia t lymphocyte from peripheral blood) whole cell lysate at 30 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 34 kDa,36 kDa

false

Western blot - Anti-PYCR2 antibody [EPR28825-54] (AB324352)
  • WB

Supplier Data

Western blot - Anti-PYCR2 antibody [EPR28825-54] (AB324352)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with recombinant human PYCR1 or PYCR3 by western blot.

In Western blot, Anti-6X His tag® antibody [AD1.1.10] - (ab15149) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-PYCR2 antibody [EPR28825-54] (ab324352) at 1/1000 dilution

Lane 1:

His-tagged human PYCR1 recombinant protein at 10 ng

Lane 2:

His-tagged human PYCR2 recombinant protein at 10 ng

Lane 3:

His-tagged human PYCR3 recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 34 kDa,33 kDa,29 kDa

false

Exposure time: 180s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28825-54

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Housekeeping enzyme that catalyzes the last step in proline biosynthesis. In some cell types, such as erythrocytes, its primary function may be the generation of NADP(+). Can utilize both NAD and NADP. Has higher affinity for NADP, but higher catalytic efficiency with NADH (PubMed : 2722838, PubMed : 6894153). Involved in cellular response to oxidative stress (PubMed : 25865492).
See full target information PYCR2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com