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AB103419

Anti-PYGL antibody

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(1 Publication)

Rabbit Polyclonal PYGL antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Glycogen phosphorylase, liver form.

View Alternative Names

PYGL

2 Images
Western blot - Anti-PYGL antibody (AB103419)
  • WB

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Western blot - Anti-PYGL antibody (AB103419)

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Western blot - Anti-PYGL antibody (ab103419) at 5 µg/mL

Lane 1:

PYGL transfected 293T cell lysate at 25 µg

Lane 2:

Non-transfected 293T cell lysate at 25 µg

Predicted band size: 101 kDa,102 kDa,50 kDa,51 kDa,52 kDa,58 kDa,61 kDa,62 kDa,65 kDa,68 kDa,69 kDa,74 kDa,77 kDa,88 kDa,93 kDa,97 kDa

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Western blot - Anti-PYGL antibody (AB103419)
  • WB

Unknown

Western blot - Anti-PYGL antibody (AB103419)

All lanes:

Western blot - Anti-PYGL antibody (ab103419) at 5 µg/mL

All lanes:

Human liver tissue lysate at 50 µg

Predicted band size: 127 kDa,97 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human Glycogen phosphorylase, liver form.

P06737

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PYGL also known as glycogen phosphorylase liver form is an enzyme with a monomeric mass of around 97 kDa. Mechanically PYGL catalyzes the breakdown of glycogen into glucose-1-phosphate by cleaving alpha-14-glycosidic bonds. This enzyme primarily operates in the liver where it plays a critical role in regulating glucose release into the bloodstream. PYGL's activity is tightly controlled by hormonal signals which ensure proper glucose levels.
Biological function summary

The enzyme plays a major role in maintaining energy homeostasis by mobilizing glucose from glycogen stores during fasting states. It does not function as part of a larger complex operating instead as a homodimer when activated. PYGL is critical for the liver's ability to supply glucose to other organs when dietary glucose is not available. Proper functioning of PYGL helps maintain a balance between glucose storage and release contributing to overall metabolic equilibrium.

Pathways

PYGL has a notable role in glycogenolysis a process that liberates stored glycogen as glucose-1-phosphate. This enzyme also connects with gluconeogenesis where it impacts the production of glucose from non-carbohydrate sources. In these pathways its activity often intersects with other enzymes namely phosphoglucomutase and glucose-6-phosphatase which further process the products of PYGL’s activity.

PYGL is associated with conditions such as Hers disease a glycogen storage disease caused by a deficiency in hepatic glycogen phosphorylase. This leads to excessive glycogen accumulation in the liver and resultant hypoglycemia. Additionally abnormal PYGL activity relates to type 2 diabetes where altered glycogen metabolism impacts insulin response and glucose homeostasis. In these contexts it maintains functional connections with proteins like insulin receptor and glycogen synthase which together influence carbohydrate metabolism balance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Allosteric enzyme that catalyzes the rate-limiting step in glycogen catabolism, the phosphorolytic cleavage of glycogen to produce glucose-1-phosphate, and plays a central role in maintaining cellular and organismal glucose homeostasis.
See full target information Glycogen phosphorylase, liver form
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer genomics & proteomics 20:30-39 PubMed36581340

2022

Rab27b, a Regulator of Exosome Secretion, Is Associated With Peritoneal Metastases in Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sho Nambara,Takaaki Masuda,Kosuke Hirose,Qingjiang Hu,Taro Tobo,Yuki Ozato,Junji Kurashige,Yoshiki Hiraki,Yuichi Hisamatsu,Tomohiro Iguchi,Keishi Sugimachi,Eiji Oki,Tomoharu Yoshizumi,Koshi Mimori
View all publications
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Product promise

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