AB223788

Anti-PYGL antibody

Name: Anti-PYGL antibody (ab223788) | Abcam
Brand: Abcam Limited
SKU: AB223788
Price: 545 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal PYGL antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Glycogen phosphorylase, liver form aa 700 to C-terminus.

View Alternative Names

PYGL

3 Images

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PYGL antibody (AB223788)

PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for PYGL (green) using ab223788 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYGL antibody (AB223788)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYGL antibody (AB223788)

Paraffin-embedded human liver tissue stained for PYGL using ab223788 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-PYGL antibody (AB223788)

All lanes:

Western blot - Anti-PYGL antibody (ab223788) at 1/250 dilution

All lanes:

U-251 MG (human brain glioma cell line) cell lysate

Predicted band size: 97 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Glycogen phosphorylase, liver form aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P06737

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Sheep": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PYGL also known as glycogen phosphorylase liver form is an enzyme with a monomeric mass of around 97 kDa. Mechanically PYGL catalyzes the breakdown of glycogen into glucose-1-phosphate by cleaving alpha-14-glycosidic bonds. This enzyme primarily operates in the liver where it plays a critical role in regulating glucose release into the bloodstream. PYGL's activity is tightly controlled by hormonal signals which ensure proper glucose levels.

Biological function summary

The enzyme plays a major role in maintaining energy homeostasis by mobilizing glucose from glycogen stores during fasting states. It does not function as part of a larger complex operating instead as a homodimer when activated. PYGL is critical for the liver's ability to supply glucose to other organs when dietary glucose is not available. Proper functioning of PYGL helps maintain a balance between glucose storage and release contributing to overall metabolic equilibrium.

Pathways

PYGL has a notable role in glycogenolysis a process that liberates stored glycogen as glucose-1-phosphate. This enzyme also connects with gluconeogenesis where it impacts the production of glucose from non-carbohydrate sources. In these pathways its activity often intersects with other enzymes namely phosphoglucomutase and glucose-6-phosphatase which further process the products of PYGL’s activity.

PYGL is associated with conditions such as Hers disease a glycogen storage disease caused by a deficiency in hepatic glycogen phosphorylase. This leads to excessive glycogen accumulation in the liver and resultant hypoglycemia. Additionally abnormal PYGL activity relates to type 2 diabetes where altered glycogen metabolism impacts insulin response and glucose homeostasis. In these contexts it maintains functional connections with proteins like insulin receptor and glycogen synthase which together influence carbohydrate metabolism balance.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Allosteric enzyme that catalyzes the rate-limiting step in glycogen catabolism, the phosphorolytic cleavage of glycogen to produce glucose-1-phosphate, and plays a central role in maintaining cellular and organismal glucose homeostasis.

See full target information Glycogen phosphorylase, liver form

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal for immunotherapy of cancer 13: PubMed40954077

2025

LncRNA-CTD suppresses metastasis and immune evasion by modulating snail1 and MHC-I expression in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Xu,Huisi Qiu,Yuezhang Sun,Hong Jin,Siyu Yan,Fangmei Zhang,Ying Song,Guopei Zheng,Dongjiang Liao,Xiaoli Wei,Liyun Luo

Scientific reports 14:13670 PubMed38871968

2024

PCK1-mediated glycogenolysis facilitates ROS clearance and chemotherapy resistance in cervical cancer stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xinxin Chen,Nan Yang,Ying Wang,Shuang Yang,Yuanhong Peng

The Journal of clinical investigation 133: PubMed38099490

2023

Aryl hydrocarbon receptor sulfenylation promotes glycogenolysis and rescues cancer chemoresistance.

Applications

Unspecified application

Species

Unspecified reactive species

Nannan Zhou,Jie Chen,Zheng Ling,Chaoqi Zhang,Yabo Zhou,Dianheng Wang,Li Zhou,Zhenfeng Wang,Nan Sun,Xin Wang,Huafeng Zhang,Ke Tang,Jingwei Ma,Jiadi Lv,Bo Huang

Cell reports 42:112578 PubMed37267108

2023

Mutant IDH regulates glycogen metabolism from early cartilage development to malignant chondrosarcoma formation.

Applications

Unspecified application

Species

Unspecified reactive species

Sinthu Pathmanapan,Raymond Poon,Tomasa Barrientos De Renshaw,Puviindran Nadesan,Makoto Nakagawa,Gireesh A Seesankar,Adrian Kwan Ho Loe,Hongyuan H Zhang,Joan J Guinovart,Jordi Duran,Christopher B Newgard,Jay S Wunder,Benjamin A Alman

Carbohydrate polymers 243:116435 PubMed32532388

2020

Metformin and Berberine suppress glycogenolysis by inhibiting glycogen phosphorylase and stabilizing the molecular structure of glycogen in db/db mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaocui Liu,Kaiping Wang,Jing Zhou,Mitchell A Sullivan,Yage Liu,Robert G Gilbert,Bin Deng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PYGL antibody

Immunocytochemistry/ Immunofluorescence - Anti-PYGL antibody (AB223788)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYGL antibody (AB223788)

Western blot - Anti-PYGL antibody (AB223788)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise