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Rabbit Recombinant Monoclonal PYGL phospho S430 antibody. Suitable for WB, IP, Dot and reacts with Human, Mouse, Rat, Synthetic peptide samples.

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Images

Immunoprecipitation - Anti-PYGL (phospho S430) + PYGM (phospho S430) antibody [EPR20852-26] (AB314428), expandable thumbnail
  • Immunoprecipitation - Anti-PYGL (phospho S430) + PYGM (phospho S430) antibody [EPR20852-26] (AB314428), expandable thumbnail
  • Immunoprecipitation - Anti-PYGL (phospho S430) + PYGM (phospho S430) antibody [EPR20852-26] (AB314428), expandable thumbnail
  • Dot Blot - Anti-PYGL (phospho S430) + PYGM (phospho S430) antibody [EPR20852-26] (AB314428), expandable thumbnail
  • Western blot - Anti-PYGL (phospho S430) + PYGM (phospho S430) antibody [EPR20852-26] (AB314428), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPDotFlow Cyt (Intra)IHC-PICC/IF
Human
Tested
Tested
Expected
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Expected
Not recommended
Not recommended
Not recommended
Rat
Tested
Tested
Expected
Not recommended
Not recommended
Not recommended
Synthetic peptide
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Species
Mouse
Dilution info
1/30
Notes

-

Species
Rat
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Human, Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Synthetic peptide
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Synthetic peptide
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Synthetic peptide
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Allosteric enzyme that catalyzes the rate-limiting step in glycogen catabolism, the phosphorolytic cleavage of glycogen to produce glucose-1-phosphate, and plays a central role in maintaining cellular and organismal glucose homeostasis.

Additional Targets

Pygm phospho S430

Alternative names

Pygl

Recommended products

Rabbit Recombinant Monoclonal PYGL phospho S430 antibody. Suitable for WB, IP, Dot and reacts with Human, Mouse, Rat, Synthetic peptide samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR20852-26
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PYGL (phospho S430) and PYGM (phospho S430) are phosphorylated forms of the enzymes glycogen phosphorylase liver (PYGL) and muscle (PYGM). These enzymes play fundamental roles in glycogen metabolism catalyzing the breakdown of glycogen into glucose-1-phosphate which can then be converted to glucose-6-phosphate for energy production. The phosphorylated form at the serine 430 residue is significant for regulation. PYGL is predominantly expressed in liver tissues while PYGM is found mainly in skeletal muscle. The mass of PYGL is approximately 97 kDa and PYGM is around 97 kDa as well.

Biological function summary

Glycogen phosphorylases participate in the glycogenolysis process providing energy during fasting or muscular activity. The enzymes operate as dimers or tetramers forming more active configurations when phosphorylated. PYGL helps maintain blood sugar levels during fasting by releasing glucose from liver glycogen stores. PYGM facilitates glucose availability during muscle contraction. Both forms influence energy balance and metabolic responses.

Pathways

Both PYGL and PYGM are central to carbohydrate metabolism and energy homeostasis. They are key components of the glycogen breakdown pathway which intersects with glycolysis. These enzymes interact with proteins like AMP-activated protein kinase (AMPK) which influences their activity through phosphorylation status. This regulation connects to pathways like the insulin signaling pathway controlling glucose production and uptake.

Associated diseases and disorders

Glycogen phosphorylase activity associates with conditions like McArdle disease and Hers disease. McArdle disease involves a deficiency of PYGM leading to muscle pain and exercise intolerance. Conversely Hers disease results from deficient PYGL activity causing hypoglycemia and liver enlargement. In these conditions the malfunction of either PYGL or PYGM disrupts normal glycogen metabolism linking them to altered energy states and organ function.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunoprecipitation - Anti-PYGL (phospho S430) + PYGM (phospho S430) antibody [EPR20852-26] (ab314428), expandable thumbnail

    Immunoprecipitation - Anti-PYGL (phospho S430) + PYGM (phospho S430) antibody [EPR20852-26] (ab314428)

    PYGL (phospho S430) + PYGM (phospho S430) was immunoprecipitated from 0.35 mg Rat liver tissue lysate with ab314428 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314428 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/1000 dilution.

    Lane 1: Rat liver tissue lysate

    Lane 2: ab314428 at 1/30 IP in Rat liver tissue lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab314428 in rat liver tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    All lanes: Immunoprecipitation - Anti-PYGL (phospho S430) + PYGM (phospho S430) antibody [EPR20852-26] (ab314428) at 1/1000 dilution

    Lane 1: Rat liver tissue lysate at 10 µg

    Lane 2: ab314428 at 1/30 IP in Rat liver tissue lysate at 10 µg

    Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 in rat liver tissue lysate at 10 µg

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Developed using the ECL technique.

    Exposure time: 67s

  • Immunoprecipitation - Anti-PYGL (phospho S430) + PYGM (phospho S430) antibody [EPR20852-26] (ab314428), expandable thumbnail

    Immunoprecipitation - Anti-PYGL (phospho S430) + PYGM (phospho S430) antibody [EPR20852-26] (ab314428)

    PYGL (phospho S430) + PYGM (phospho S430) was immunoprecipitated from 0.35 mg Mouse liver tissue lysate with ab314428 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314428 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse liver tissue lysate

    Lane 2: ab314428 at 1/30 IP in Mouse liver tissue lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab314428 in mouse liver tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    All lanes: Immunoprecipitation - Anti-PYGL (phospho S430) + PYGM (phospho S430) antibody [EPR20852-26] (ab314428) at 1/1000 dilution

    Lane 1: Mouse liver tissue lysate at 10 µg

    Lane 2: ab314428 at 1/30 IP in mouse liver tissue lysate at 10 µg

    Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 in mouse liver tissue lysate at 10 µg

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Developed using the ECL technique.

    Exposure time: 180s

  • Immunoprecipitation - Anti-PYGL (phospho S430) + PYGM (phospho S430) antibody [EPR20852-26] (ab314428), expandable thumbnail

    Immunoprecipitation - Anti-PYGL (phospho S430) + PYGM (phospho S430) antibody [EPR20852-26] (ab314428)

    PYGL (phospho S430) + PYGM (phospho S430) was immunoprecipitated from 0.35 mg Human liver tissue lysate with ab314428 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314428 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/1000 dilution.

    Lane 1: Human liver tissue lysate

    Lane 2: ab314428 at 1/30 IP in Human liver tissue lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab314428 in human liver tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    All lanes: Immunoprecipitation - Anti-PYGL (phospho S430) + PYGM (phospho S430) antibody [EPR20852-26] (ab314428) at 1/1000 dilution

    Lane 1: Human liver tissue lysate at 10 µg

    Lane 2: ab314428 at 1/30 IP in Human liver tissue lysate at 10 µg

    Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 in human liver tissue lysate at 10 µg

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Developed using the ECL technique.

    Exposure time: 67s

  • Dot Blot - Anti-PYGL (phospho S430) + PYGM (phospho S430) antibody [EPR20852-26] (ab314428), expandable thumbnail

    Dot Blot - Anti-PYGL (phospho S430) + PYGM (phospho S430) antibody [EPR20852-26] (ab314428)

    Dot blot analysis of PYGL (phospho S430) + PYGM (phospho S430) using ab314428 at 1:1000 (0.526 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
    Exposure time: 180 seconds
    Blocking and diluting buffer and concentration: 5% NFDM/TBST

  • Western blot - Anti-PYGL (phospho S430) + PYGM (phospho S430) antibody [EPR20852-26] (ab314428), expandable thumbnail

    Western blot - Anti-PYGL (phospho S430) + PYGM (phospho S430) antibody [EPR20852-26] (ab314428)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-PYGL (phospho S430) + PYGM (phospho S430) antibody [EPR20852-26] (ab314428) at 1/1000 dilution

    Lane 1: Mouse liver tissue lysate (untreated membrane) at 20 µg

    Lane 2: Rat liver tissue lysate (untreated membrane) at 20 µg

    Lane 3: Mouse liver tissue lysate (Alkaline phosphatase treated membrane) at 20 µg

    Lane 4: Rat liver tissue lysate (Alkaline phosphatase treated membrane) at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Developed using the ECL technique.

    Observed band size: 97 kDa

    Exposure time: 180s

  • Western blot - Anti-PYGL (phospho S430) + PYGM (phospho S430) antibody [EPR20852-26] (ab314428), expandable thumbnail

    Western blot - Anti-PYGL (phospho S430) + PYGM (phospho S430) antibody [EPR20852-26] (ab314428)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    The identities of the additional bands at 150 kDa and between 75 and 10 kDa is unknown.

    All lanes: Western blot - Anti-PYGL (phospho S430) + PYGM (phospho S430) antibody [EPR20852-26] (ab314428) at 1/1000 dilution

    Lane 1: Human liver tissue lysate at 20 µg

    Lane 2: Mouse liver tissue lysate at 20 µg

    Lane 3: Mouse skeletal muscle tissue lysate at 20 µg

    Lane 4: Rat skeletal muscle tissue lysate at 20 µg

    Lane 5: Rat liver tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Observed band size: 97 kDa

    Exposure time: 180s

  • Western blot - Anti-PYGL (phospho S430) + PYGM (phospho S430) antibody [EPR20852-26] (ab314428), expandable thumbnail

    Western blot - Anti-PYGL (phospho S430) + PYGM (phospho S430) antibody [EPR20852-26] (ab314428)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    The identities of the additional bands at 150 kDa and between 75 and 10 kDa is unknown

    All lanes: Western blot - Anti-PYGL (phospho S430) + PYGM (phospho S430) antibody [EPR20852-26] (ab314428) at 1/1000 dilution

    Lane 1: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: HaCaT (human skin keratinocyte) whole cell lysate at 20 µg

    Lane 3: A-204 (human muscle rhabdomyosarcoma cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Observed band size: 97 kDa

    Exposure time: 125s

  • Western blot - Anti-PYGL (phospho S430) + PYGM (phospho S430) antibody [EPR20852-26] (ab314428), expandable thumbnail

    Western blot - Anti-PYGL (phospho S430) + PYGM (phospho S430) antibody [EPR20852-26] (ab314428)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-PYGL (phospho S430) + PYGM (phospho S430) antibody [EPR20852-26] (ab314428) at 1/1000 dilution

    Lane 1: Human liver tissue lysate (untreated membrane) at 20 µg

    Lane 2: Human liver tissue lysate (Alkaline phosphatase treated membrane) at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Observed band size: 97 kDa

    Exposure time: 180s

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Product protocols

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