JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB231963

Anti-PYGM antibody

Be the first to review this product! Submit a review

|

(6 Publications)

Rabbit Polyclonal PYGM antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Pygm aa 1-200.

View Alternative Names

Myophosphorylase, PYGM

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYGM antibody (AB231963)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYGM antibody (AB231963)

Formalin-fixed, paraffin-embedded mouse skeletal muscle tissue stained for PYGM using ab231963 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Western blot - Anti-PYGM antibody (AB231963)
  • WB

Supplier Data

Western blot - Anti-PYGM antibody (AB231963)

Recombinant fragment (His-tag) corresponding to Mouse PYGM aa 11-187, 22kDa.

All lanes:

Western blot - Anti-PYGM antibody (ab231963) at 2 µg/mL

All lanes:

Recombinant mouse PYGM protein

Predicted band size: 97 kDa

false

Western blot - Anti-PYGM antibody (AB231963)
  • WB

Supplier Data

Western blot - Anti-PYGM antibody (AB231963)

All lanes:

Western blot - Anti-PYGM antibody (ab231963) at 2 µg/mL

All lanes:

Mouse skeletal muscle lysate

Predicted band size: 111 kDa,77 kDa,97 kDa

false

Western blot - Anti-PYGM antibody (AB231963)
  • WB

Supplier Data

Western blot - Anti-PYGM antibody (AB231963)

All lanes:

Western blot - Anti-PYGM antibody (ab231963) at 2 µg/mL

All lanes:

Mouse liver lysate

Predicted band size: 97 kDa

false

Western blot - Anti-PYGM antibody (AB231963)
  • WB

Supplier Data

Western blot - Anti-PYGM antibody (AB231963)

All lanes:

Western blot - Anti-PYGM antibody (ab231963) at 2 µg/mL

All lanes:

Rat skeletal muscle lysate

Predicted band size: 97 kDa

false

Western blot - Anti-PYGM antibody (AB231963)
  • WB

Supplier Data

Western blot - Anti-PYGM antibody (AB231963)

All lanes:

Western blot - Anti-PYGM antibody (ab231963) at 2 µg/mL

All lanes:

HepG2 (human liver hepatocellular carcinoma cell line) cell lysate

Predicted band size: 97 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Mouse Pygm aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q9WUB3

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PYGM also known as muscle glycogen phosphorylase or myophosphorylase is an enzyme with a molecular mass of around 97 kDa. This enzyme plays a mechanical role in glycogen metabolism by catalyzing the breakdown of glycogen to glucose-1-phosphate. PYGM is particularly expressed in skeletal muscles where it aids in mobilizing stored energy during periods of increased demand such as exercise.
Biological function summary

The function of PYGM is critical in energy homeostasis within muscle tissue. This enzyme forms part of the glycogen phosphorylase complex which facilitates rapid energy release by converting glycogen into glucose-1-phosphate. This release is important for maintaining adequate levels of glucose for muscle contraction during activity or stress. Its activity is modulated by various factors including allosteric changes and phosphorylation processes ensuring that glucose metabolism aligns with the cellular energy requirements.

Pathways

PYGM integrates mainly into the glycolytic pathway where it provides glucose-1-phosphate as a substrate for glycolysis enhancing ATP production. The enzyme also plays an important role in the glycogenolysis pathway orchestrating energy release from glycogen stores. PYGM functions in connection with related proteins such as glycogen debranching enzyme and phosphoglucomutase working in concert to maintain energy balance and inform the overall metabolic response during physical exertion.

Defects in PYGM relate to McArdle disease a metabolic muscle disorder characterized by exercise intolerance and myoglobinuria. Deficiencies in PYGM impair glycogen breakdown in muscles leading to reduced availability of glucose for energy and resulting in muscle fatigue and pain. This disorder often links with metabolic consequences affecting enzymes involved in glucose handling such as muscle adenylate kinase highlighting the interconnected nature of metabolic pathways and disease pathology.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Allosteric enzyme that catalyzes the rate-limiting step in glycogen catabolism, the phosphorolytic cleavage of glycogen to produce glucose-1-phosphate, and plays a central role in maintaining cellular and organismal glucose homeostasis.
See full target information PYGM
style
left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:2821 PubMed40121188

2025

Smoking aggravates neovascular age-related macular degeneration via Sema4D-PlexinB1 axis-mediated activation of pericytes.

Applications

Unspecified application

Species

Unspecified reactive species

Kai He,Xue Dong,Tianjing Yang,Ziqi Li,Yuming Liu,Jing He,Meng Wu,Selena Wei-Zhang,Parhat Kaysar,Bohao Cui,Xueming Yao,Li Zhang,Wei Zhou,Heping Xu,Jun Wei,Qiang Liu,Junhao Hu,Xiaohong Wang,Hua Yan

CNS neuroscience & therapeutics 30:e70065 PubMed39350328

2024

Comprehensive Proteomic Analysis of Dysferlinopathy Unveiling Molecular Mechanisms and Biomarkers Linked to Pathological Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Di Wang,Xin-Yi Liu,Qi-Fang He,Fu-Ze Zheng,Long Chen,Ying Zheng,Ming-Hui Zeng,Yu-Hua Lin,Xin Lin,Hai-Zhu Chen,Min-Ting Lin,Ning Wang,Zhi-Qiang Wang,Feng Lin

Reproduction (Cambridge, England) 168: PubMed39226129

2024

Membrane progesterone receptors mediate progesterone-stimulated glycogenolysis in the bovine uterine epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Malia D Berg,Matthew Dean

PloS one 19:e0295964 PubMed38289946

2024

A novel genetic model provides a unique perspective on the relationship between postexercise glycogen concentration and increases in the abundance of key metabolic proteins after acute exercise.

Applications

Unspecified application

Species

Unspecified reactive species

Seong Eun Kwak,Amy Zheng,Edward B Arias,Haiyan Wang,Xiufang Pan,Yongping Yue,Dongsheng Duan,Gregory D Cartee

Molecular reproduction and development 89:431-440 PubMed35842832

2022

Endometrial glycogen metabolism during early pregnancy in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ziting Chen,Kassandra Sandoval,Matthew Dean

Frontiers in microbiology 11:557039 PubMed33329421

2020

Glycogen Phosphorylase: A Drug Target of Amino Alcohols in , Predicted by a Computer-Aided Method.

Applications

Unspecified application

Species

Unspecified reactive species

Congshan Liu,Jianhai Yin,Wei Hu,Haobing Zhang
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB207442

Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549]

RabMAb|Recombinant

Immunoprecipitation - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (AB207442)

  • 0
  • 0
Primary Antibodies

AB268020

Anti-Fibronectin antibody [EPR23110-46]

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR23110-46] (AB268020)

  • 4
  • 5
Primary Antibodies

AB76302

Anti-NF-kB p65 (phospho S536) antibody [EP2294Y]

20ul selling size|RabMAb|Recombinant

Immunoprecipitation - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (AB76302)

  • 4
  • 7

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com