JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB128174

Anti-PYGO1 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal PYGO1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Pygopus homolog 1 aa 300 to C-terminus.

View Alternative Names

Pygopus homolog 1, PYGO1

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYGO1 antibody (AB128174)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYGO1 antibody (AB128174)

ab128174, at 1/250 dilution, staining PYGO1 in formalin-fixed paraffin-embedded Human colon carcinoma tissue by Immunohistochemistry.
Detection : DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human Pygopus homolog 1 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y3Y4

style
left-column

Alternative Products

Primary Antibodies

AB95072

Anti-PYGO1 antibody

Immunoprecipitation - Anti-PYGO1 antibody (AB95072)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB37305

Anti-BCL9 antibody

Western blot - Anti-BCL9 antibody (AB37305)

  • 3
  • 2
Primary Antibodies

AB314440

Anti-BCL9L antibody [EPR27491-35]

20ul selling size|KO Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-BCL9L antibody [EPR27491-35] (AB314440)

  • 0
  • 0
Primary Antibodies

AB288308

Anti-PD-L2 antibody [EPR25200-50] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L2 antibody [EPR25200-50] - BSA and Azide free (AB288308)

  • 0
  • 0
Primary Antibodies

AB315280

Anti-PDE3A antibody [EPR28228-6] - BSA and Azide free

Recombinant|RabMAb

Western blot - Anti-PDE3A antibody [EPR28228-6] - BSA and Azide free (AB315280)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Ferret": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab128174 is affinity purified using an epitope specific to PYGO1 immobilized on solid support.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: 99% Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PYGO1 also known as Pygopus homolog 1 functions as a co-activator in Wnt signaling playing an important role in transcriptional activation. It comprises a protein mass of approximately 44 kDa. The expression of PYGO1 occurs in various human tissues with increased levels observed in the brain and testes. As a component of the β-catenin destruction complex PYGO1 influences the stabilization and nuclear localization of β-catenin which is essential for its activity.
Biological function summary

PYGOPUS 1 acts as a significant mediator in the Wnt/β-catenin signaling pathway primarily facilitating gene transcription by interacting with both β-catenin and other transcriptional co-activators. It belongs to a multi-protein complex that modulates chromatin remodeling and transcription. PYGO1 enhances the function of the TCF/LEF family of transcription factors impacting cell fate decisions during developmental processes.

Pathways

PYGO1 significantly impacts the Wnt signaling pathway which plays a critical role in embryonic development and cell proliferation. In collaboration with proteins like β-catenin and BCL9 PYGO1 aids in the regulation of target gene expression. Another important pathway involving PYGO1 is the Notch signaling pathway where it can indirectly influence cellular differentiation and apoptosis through cross-talk and interplay with Wnt pathways highlighting its integrative role in cellular signaling.

PYGO1 shows associations with cancer particularly colorectal cancer due to its role in aberrant Wnt signaling. Abnormal activation of the Wnt/β-catenin pathway where PYGO1 is actively involved contributes to tumorigenesis and cancer progression. Additionally in rheumatoid arthritis mutations or altered expressions of PYGO1 may influence inflammatory responses potentially in coordination with proteins such as β-catenin indicating its broader implication in inflammatory and autoimmune conditions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Involved in signal transduction through the Wnt pathway.
See full target information Pygopus homolog 1
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 7:426 PubMed38589567

2024

Targeting TRIP13 in favorable histology Wilms tumor with nuclear export inhibitors synergizes with doxorubicin.

Applications

Unspecified application

Species

Unspecified reactive species

Karuna Mittal,Garrett W Cooper,Benjamin P Lee,Yongdong Su,Katie T Skinner,Jenny Shim,Hunter C Jonus,Won Jun Kim,Mihir Doshi,Diego Almanza,Bryan D Kynnap,Amanda L Christie,Xiaoping Yang,Glenn S Cowley,Brittaney A Leeper,Christopher L Morton,Bhakti Dwivedi,Taylor Lawrence,Manali Rupji,Paula Keskula,Stephanie Meyer,Catherine M Clinton,Manoj Bhasin,Brian D Crompton,Yuen-Yi Tseng,Jesse S Boehm,Keith L Ligon,David E Root,Andrew J Murphy,David M Weinstock,Prafulla C Gokhale,Jennifer M Spangle,Miguel N Rivera,Elizabeth A Mullen,Kimberly Stegmaier,Kelly C Goldsmith,William C Hahn,Andrew L Hong
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com