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Rabbit Polyclonal PYGO2 antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Rat, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PYGO2 aa 1-400.

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Images

Immunoprecipitation - Anti-PYGO2 antibody (AB155262), expandable thumbnail
  • Western blot - Anti-PYGO2 antibody (AB155262), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYGO2 antibody (AB155262), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYGO2 antibody (AB155262), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYGO2 antibody (AB155262), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)

Form
Liquid
Clonality
Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human PYGO2 aa 1-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BRQ0

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWB
Human
Tested
Tested
Expected
Mouse
Tested
Expected
Tested
Rat
Tested
Expected
Expected

Tested
Tested

Species
Mouse
Dilution info
1/100.00000 - 1/1000.00000
Notes

Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/100.00000 - 1/1000.00000
Notes

Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/100.00000 - 1/1000.00000
Notes

Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500.00000 - 1/3000.00000
Notes

-

Expected
Expected

Species
Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Involved in signal transduction through the Wnt pathway.

Alternative names

Recommended products

Rabbit Polyclonal PYGO2 antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Rat, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PYGO2 aa 1-400.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Polyclonal
Immunogen
  • Recombinant Fragment Protein within Human PYGO2 aa 1-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BRQ0
Purification technique
Affinity purification Immunogen
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PYGO2 also known as Pygopus family PHD finger 2 is a nuclear protein involved in gene transcription. This protein has a molecular mass of approximately 45 kDa. PYGO2 localizes mainly in the nucleus and it is expressed in several tissues particularly in those with high proliferation demands like embryonic tissues or certain tumor cells. Its role in transcription is linked to its ability to bind to histones and recruit other transcriptional cofactors facilitating the assembly of transcriptional machinery at specific genomic sites.

Biological function summary

This protein plays a significant role in gene expression regulation functioning as a component of the Wnt signaling pathway. PYGO2 works in a transcriptional complex with other proteins including BCL9 to regulate gene transcription that is important for cell proliferation and differentiation. The interaction with BCL9 is critical for stabilizing the multiprotein complexes involved in transcriptional activation indicating the importance of PYGO2 in developmental processes.

Pathways

PYGO2 serves a critical function in the canonical Wnt signaling pathway which is vital in controlling the expression of target genes related to cell growth and development. The Wnt pathway requires the cooperation of PYGO2 with LEF/TCF transcription factors and β-catenin allowing them to operate as an activated complex. The interaction with β-catenin highlights PYGO2's role in translating extracellular Wnt signals into gene transcription responses within the cell.

Associated diseases and disorders

PYGO2 has associations with several cancer types particularly those with aberrant Wnt signaling such as colorectal cancer. Disruptions in PYGO2 expression or function can lead to dysregulation of gene expression linked to tumorigenesis. Another potential link exists with breast cancer where altered expression of PYGO2 and its interaction with β-catenin can influence cancer cell proliferation and invasion. Understanding the specific role of PYGO2 in these contexts might offer insights into potential therapeutic targets for cancer treatment.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunoprecipitation - Anti-PYGO2 antibody (ab155262), expandable thumbnail

    Immunoprecipitation - Anti-PYGO2 antibody (ab155262)

    Immunoprecipitation of PYGO2 from SW480 whole cell lysate (5 X 107 cells) using 1 ug of ab155262. The precipitated PYGO2 was detected using ab155262 diluted at 1:15000.
    Lane 1: IP-IgG
    Lane 2: IP-PYGO2

    All lanes: Immunoprecipitation - Anti-PYGO2 antibody (ab155262) at 1/15000 dilution

    Lane 1: IP negative control - SW480 whole cell lysate, corresponding to 5*107 cells, incubated with a IgG.

    Lane 2: SW480 whole cell lysate, corresponding to 5*107 cells, immunoprecipitated with 1 µg of ab155262

    Predicted band size: 41 kDa

  • Western blot - Anti-PYGO2 antibody (ab155262), expandable thumbnail

    Western blot - Anti-PYGO2 antibody (ab155262)

    10% SDS PAGE

    All lanes: Western blot - Anti-PYGO2 antibody (ab155262) at 1/1000 dilution

    All lanes: mouse NIH 3T3 whole cell lysate at 30 µg

    Predicted band size: 37 kDa, 41 kDa, 54 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYGO2 antibody (ab155262), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYGO2 antibody (ab155262)

    4% paraformaldehyde-fixed HCT 116 (human colorectal carcinoma cell line) cells labeling PYGO2 using ab155262 at 1/500 dilution (green) in ICC/IF.

    The nuclear counter stain is Hoechst 33342 (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYGO2 antibody (ab155262), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYGO2 antibody (ab155262)

    Paraffin-embedded rat intestine tissue labeleing PYGO2 using ab155262 at 1/500 dilution in immunohistochemical analysis.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYGO2 antibody (ab155262), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYGO2 antibody (ab155262)

    Paraffin-embedded mouse stomach tissue labeleing PYGO2 using ab155262 at 1/500 dilution in immunohistochemical analysis.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYGO2 antibody (ab155262), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYGO2 antibody (ab155262)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung adenocarcinoma tissue, labeling PYGO2 using ab155262 at a 1/500 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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