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AB155262

Anti-PYGO2 antibody

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(2 Publications)

Rabbit Polyclonal PYGO2 antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PYGO2 aa 1-400.

View Alternative Names

PP7910, PYGO2, Pygopus homolog 2

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYGO2 antibody (AB155262)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYGO2 antibody (AB155262)

4% paraformaldehyde-fixed HCT 116 (human colorectal carcinoma cell line) cells labeling PYGO2 using ab155262 at 1/500 dilution (green) in ICC/IF.

The nuclear counter stain is Hoechst 33342 (blue).

Immunoprecipitation - Anti-PYGO2 antibody (AB155262)
  • IP

Unknown

Immunoprecipitation - Anti-PYGO2 antibody (AB155262)

Immunoprecipitation of PYGO2 from SW480 whole cell lysate (5 X 107 cells) using 1 ug of ab155262. The precipitated PYGO2 was detected using ab155262 diluted at 1 : 15000.
Lane 1 : IP-IgG
Lane 2 : IP-PYGO2

All lanes:

Immunoprecipitation - Anti-PYGO2 antibody (ab155262) at 1/15000 dilution

Lane 1:

IP negative control - SW480 whole cell lysate, corresponding to 5*10<sup>7</sup> cells, incubated with a IgG

Lane 2:

SW480 whole cell lysate, corresponding to 5*10<sup>7</sup> cells, immunoprecipitated with 1 µg of ab155262

Predicted band size: 41 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYGO2 antibody (AB155262)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYGO2 antibody (AB155262)

Paraffin-embedded rat intestine tissue labeleing PYGO2 using ab155262 at 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 minutes.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYGO2 antibody (AB155262)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYGO2 antibody (AB155262)

Paraffin-embedded mouse stomach tissue labeleing PYGO2 using ab155262 at 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 minutes.

Western blot - Anti-PYGO2 antibody (AB155262)
  • WB

Supplier Data

Western blot - Anti-PYGO2 antibody (AB155262)

Samples were separated by 10% SDS PAGE. The signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-PYGO2 antibody (ab155262) at 1/500 dilution

Lane 1:

Huh-7 whole cell extracts at 30 µg

Lane 2:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 41 kDa

true

Western blot - Anti-PYGO2 antibody (AB155262)
  • WB

Supplier Data

Western blot - Anti-PYGO2 antibody (AB155262)

Samples were separated by 10% SDS PAGE.

All lanes:

Western blot - Anti-PYGO2 antibody (ab155262) at 1/1000 dilution

All lanes:

NIH3T3 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 41 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IP, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PYGO2 aa 1-400. The exact immunogen used to generate this antibody is proprietary information.

Q9BRQ0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PYGO2 also known as Pygopus family PHD finger 2 is a nuclear protein involved in gene transcription. This protein has a molecular mass of approximately 45 kDa. PYGO2 localizes mainly in the nucleus and it is expressed in several tissues particularly in those with high proliferation demands like embryonic tissues or certain tumor cells. Its role in transcription is linked to its ability to bind to histones and recruit other transcriptional cofactors facilitating the assembly of transcriptional machinery at specific genomic sites.
Biological function summary

This protein plays a significant role in gene expression regulation functioning as a component of the Wnt signaling pathway. PYGO2 works in a transcriptional complex with other proteins including BCL9 to regulate gene transcription that is important for cell proliferation and differentiation. The interaction with BCL9 is critical for stabilizing the multiprotein complexes involved in transcriptional activation indicating the importance of PYGO2 in developmental processes.

Pathways

PYGO2 serves a critical function in the canonical Wnt signaling pathway which is vital in controlling the expression of target genes related to cell growth and development. The Wnt pathway requires the cooperation of PYGO2 with LEF/TCF transcription factors and β-catenin allowing them to operate as an activated complex. The interaction with β-catenin highlights PYGO2's role in translating extracellular Wnt signals into gene transcription responses within the cell.

PYGO2 has associations with several cancer types particularly those with aberrant Wnt signaling such as colorectal cancer. Disruptions in PYGO2 expression or function can lead to dysregulation of gene expression linked to tumorigenesis. Another potential link exists with breast cancer where altered expression of PYGO2 and its interaction with β-catenin can influence cancer cell proliferation and invasion. Understanding the specific role of PYGO2 in these contexts might offer insights into potential therapeutic targets for cancer treatment.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in signal transduction through the Wnt pathway.
See full target information PYGO2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer research 80:3631-3648 PubMed32586983

2020

A Pygopus 2-Histone Interaction Is Critical for Cancer Cell Dedifferentiation and Progression in Malignant Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Meera Saxena,Ravi K R Kalathur,Natalia Rubinstein,Andrea Vettiger,Nami Sugiyama,Melanie Neutzner,Mairene Coto-Llerena,Venkatesh Kancherla,Caner Ercan,Salvatore Piscuoglio,Jonas Fischer,Ernesta Fagiani,Claudio Cantù,Konrad Basler,Gerhard Christofori

Nature communications 8:15663 PubMed28580927

2017

The ATP-binding cassette transporter A1 regulates phosphoantigen release and Vγ9Vδ2 T cell activation by dendritic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Barbara Castella,Joanna Kopecka,Patrizia Sciancalepore,Giorgia Mandili,Myriam Foglietta,Nico Mitro,Donatella Caruso,Francesco Novelli,Chiara Riganti,Massimo Massaia
View all publications
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