AB109001

Anti-PYGO2 antibody [EPR2024(2)]

Name: Anti-PYGO2 antibody [EPR2024(2)] (ab109001) | Abcam
Brand: Abcam Limited
SKU: AB109001
Price: 4680 USD
Availability: InStock
Rating: 4 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(6 Publications)

Rabbit Recombinant Monoclonal PYGO2 antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications.

View Alternative Names

PP7910, PYGO2, Pygopus homolog 2

1 Images

Unknown

Western blot - Anti-PYGO2 antibody [EPR2024(2)] (AB109001)

All lanes:

Western blot - Anti-PYGO2 antibody [EPR2024(2)] (ab109001) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Human ovary cancer lysate at 10 µg

Lane 4:

NCCIT cell lysate at 10 µg

Predicted band size: 41 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2024(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PYGO2 also known as Pygopus family PHD finger 2 is a nuclear protein involved in gene transcription. This protein has a molecular mass of approximately 45 kDa. PYGO2 localizes mainly in the nucleus and it is expressed in several tissues particularly in those with high proliferation demands like embryonic tissues or certain tumor cells. Its role in transcription is linked to its ability to bind to histones and recruit other transcriptional cofactors facilitating the assembly of transcriptional machinery at specific genomic sites.

Biological function summary

This protein plays a significant role in gene expression regulation functioning as a component of the Wnt signaling pathway. PYGO2 works in a transcriptional complex with other proteins including BCL9 to regulate gene transcription that is important for cell proliferation and differentiation. The interaction with BCL9 is critical for stabilizing the multiprotein complexes involved in transcriptional activation indicating the importance of PYGO2 in developmental processes.

Pathways

PYGO2 serves a critical function in the canonical Wnt signaling pathway which is vital in controlling the expression of target genes related to cell growth and development. The Wnt pathway requires the cooperation of PYGO2 with LEF/TCF transcription factors and β-catenin allowing them to operate as an activated complex. The interaction with β-catenin highlights PYGO2's role in translating extracellular Wnt signals into gene transcription responses within the cell.

PYGO2 has associations with several cancer types particularly those with aberrant Wnt signaling such as colorectal cancer. Disruptions in PYGO2 expression or function can lead to dysregulation of gene expression linked to tumorigenesis. Another potential link exists with breast cancer where altered expression of PYGO2 and its interaction with β-catenin can influence cancer cell proliferation and invasion. Understanding the specific role of PYGO2 in these contexts might offer insights into potential therapeutic targets for cancer treatment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in signal transduction through the Wnt pathway.

See full target information PYGO2

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 24:7353-7369 PubMed32558224

2020

microRNA-128-3p overexpression inhibits breast cancer stem cell characteristics through suppression of Wnt signalling pathway by down-regulating NEK2.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanwen Chen,Nian Wu,Lei Liu,Huaying Dong,Xinao Liu

American journal of cancer research 8:2076-2087 PubMed30416857

2018

Abnormal expression of YEATS4 associates with poor prognosis and promotes cell proliferation of hepatic carcinoma cell by regulation the TCEA1/DDX3 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Song You,Fuqiang Wang,Qing Hu,Pengtao Li,Changmao Zhang,Yaqi Yu,Yi Zhang,Qiu Li,Qing Bao,Pingguo Liu,Jie Li

Oncotarget 6:11074-86 PubMed25871475

2015

Pygopus-2 promotes invasion and metastasis of hepatic carcinoma cell by decreasing E-cadherin expression.

Applications

IHC, WB

Species

Unspecified reactive species, Unspecified reactive species

Sheng Zhang,Jie Li,Pingguo Liu,Jianfeng Xu,Wenxiu Zhao,Chengrong Xie,Zhenyu Yin,Xiaomin Wang

Histopathology 67:176-84 PubMed25545771

2015

Abnormal nuclear expression of Pygopus-2 in human primary hepatocellular carcinoma correlates with a poor prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng Zhang,Jie Li,Fei He,Xiao-Min Wang

PloS one 9:e101969 PubMed25003333

2014

Repurposing the FDA-approved pinworm drug pyrvinium as a novel chemotherapeutic agent for intestinal polyposis.

Applications

Species

Human

Bin Li,Colin A Flaveny,Camilla Giambelli,Dennis Liang Fei,Lu Han,Brian I Hang,Feng Bai,Xin-Hai Pei,Vania Nose,Oname Burlingame,Anthony J Capobianco,Darren Orton,Ethan Lee,David J Robbins

BMC cancer 13:346 PubMed23865714

2013

Abnormal expression of Pygopus 2 correlates with a malignant phenotype in human lung cancer.

Applications

WB, IHC

Species

Unspecified reactive species, Unspecified reactive species

Yang Liu,Qian-Ze Dong,Si Wang,Chang-Qing Fang,Yuan Miao,Liang Wang,Ming-Zhu Li,En-Hua Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PYGO2 antibody [EPR2024(2)]

Western blot - Anti-PYGO2 antibody [EPR2024(2)] (AB109001)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise