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AB226798

Anti-PYK2 antibody

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(5 Publications)

Rabbit Polyclonal PYK2 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PTK2B.

View Alternative Names

FAK2, PYK2, RAFTK, PTK2B, Protein-tyrosine kinase 2-beta, Calcium-dependent tyrosine kinase, Calcium-regulated non-receptor proline-rich tyrosine kinase, Cell adhesion kinase beta, Focal adhesion kinase 2, Proline-rich tyrosine kinase 2, Related adhesion focal tyrosine kinase, CADTK, CAK-beta, CAKB, FADK 2

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-PYK2 antibody (AB226798)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PYK2 antibody (AB226798)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PYK2 (green) using ab226798 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

Blue : Hoechst 33342 staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 antibody (AB226798)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 antibody (AB226798)

Paraffin-embedded rat brain tissue stained for PYK2 using ab226798 at 1/500 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, IP, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human PTK2B. The exact immunogen used to generate this antibody is proprietary information.

Q14289

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Alternative Products

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PYK2 also known as Protein Tyrosine Kinase 2 Beta (PTK2B) is a protein with a mass of approximately 116 kDa. This enzyme belongs to the focal adhesion kinase (FAK) family and functions as a non-receptor protein tyrosine kinase. Mechanically PYK2 is involved in calcium-dependent signaling and is activated by increases in intracellular calcium levels. It plays a critical role in cell proliferation migration and survival. PYK2 expresses widely in neuronal tissues hematopoietic cells and various epithelial cells.
Biological function summary

PYK2 interacts with integrins and G-protein-coupled receptors (GPCRs) linking extracellular signals to intracellular pathways. It forms complexes with other signaling molecules including Rac1 and c-Src facilitating downstream signaling. These interactions contribute to cellular communication and adhesion processes especially in the central nervous system and immune cells. Through its involvement in signal transduction PYK2 supports cellular responses to environmental changes.

Pathways

PYK2 participates in the MAPK and JNK signaling pathways. These pathways are critical for transmitting signals from the cell surface to the nucleus influencing cellular growth and stress responses. PYK2 interacts closely with other proteins such as Src and FAK which help coordinate signals that regulate cellular dynamics. Through these pathways PYK2 modulates processes involved in the structural reorganization of cells and tissues.

PYK2 has been linked to neurodegenerative diseases and certain types of cancers. In Alzheimer's disease PYK2 may contribute to synaptic dysfunction and cognitive decline as it interacts with amyloid-beta peptides and tau proteins. In cancer PYK2 overexpression can promote cancer cell invasion metastasis and resistance to apoptosis often in coordination with proteins like FAK. Therefore understanding the role of PYK2 in these conditions provides insights for therapeutic developments.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Non-receptor protein-tyrosine kinase that regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading and bone remodeling. Plays a role in the regulation of the humoral immune response, and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. Required for normal macrophage polarization and migration towards sites of inflammation. Regulates cytoskeleton rearrangement and cell spreading in T-cells, and contributes to the regulation of T-cell responses. Promotes osteoclastic bone resorption; this requires both PTK2B/PYK2 and SRC. May inhibit differentiation and activity of osteoprogenitor cells. Functions in signaling downstream of integrin and collagen receptors, immune receptors, G-protein coupled receptors (GPCR), cytokine, chemokine and growth factor receptors, and mediates responses to cellular stress. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Promotes activation of NOS3. Regulates production of the cellular messenger cGMP. Promotes activation of the MAP kinase signaling cascade, including activation of MAPK1/ERK2, MAPK3/ERK1 and MAPK8/JNK1. Promotes activation of Rho family GTPases, such as RHOA and RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Acts as a scaffold, binding to both PDPK1 and SRC, thereby allowing SRC to phosphorylate PDPK1 at 'Tyr-9, 'Tyr-373', and 'Tyr-376'. Promotes phosphorylation of NMDA receptors by SRC family members, and thereby contributes to the regulation of NMDA receptor ion channel activity and intracellular Ca(2+) levels. May also regulate potassium ion transport by phosphorylation of potassium channel subunits. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ASAP1, NPHP1, KCNA2 and SHC1. Promotes phosphorylation of ASAP2, RHOU and PXN; this requires both SRC and PTK2/PYK2.
See full target information PTK2B
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:7567 PubMed37989995

2023

PTK2B promotes TBK1 and STING oligomerization and enhances the STING-TBK1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yongfang Lin,Jing Yang,Qili Yang,Sha Zeng,Jiayu Zhang,Yuanxiang Zhu,Yuxin Tong,Lin Li,Weiqi Tan,Dahua Chen,Qinmiao Sun

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e22967 PubMed37269155

2023

PTK2B regulates immune responses of neutrophils and protects mucosal inflammation in ulcerative colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Guangxi Zhou,Fengqin Zhu,Hairong Zhang,Yan Wang,Yonghong Yang,Guiyuan Jin,Yibo Wang,Guanjun Dong,Huabao Xiong

Acta pharmacologica Sinica 43:797-810 PubMed34226665

2021

Pyk2 inhibition attenuates hypoxic-ischemic brain injury in neonatal mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Zhu,Shi-Feng Chu,Ye Peng,Dan-Dan Liu,Chen Chen,Wen-Xuan Jian,Hong-Shuo Sun,Zhong-Ping Feng,Zhao Zhang,Nai-Hong Chen

Signal transduction and targeted therapy 5:139 PubMed32792487

2020

Membranous NOX5-derived ROS oxidizes and activates local Src to promote malignancy of tumor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Chen,Yan Wang,Weimin Zhang,Di Zhao,Lingyuan Zhang,Jiawen Fan,Jinting Li,Qimin Zhan

International heart journal 60:374-383 PubMed30745530

2019

Cyclin Dependent Kinase 1 (CDK1) Activates Cardiac Fibroblasts via Directly Phosphorylating Paxillin at Ser244.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Sai,Jiang Yunhan,Jian Zhao,Zhu Yu,Zhu Yun,Cao Zhezhe,Tang Fuqin,Xiao Yingbin,Ma Ruiyan
View all publications
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Product promise

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