Rabbit Recombinant Monoclonal PYK2 antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Rat, Mouse samples.
Images
![Western blot - Anti-PYK2 antibody [YE353] - BSA and Azide free (AB228477), expandable thumbnail](https://content.abcam.com/products/images/pyk2-antibody-ye353-bsa-and-azide-free-ab228477--western-blot-img51076.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PYK2 antibody [YE353] - BSA and Azide free (AB228477)")
![Immunocytochemistry/ Immunofluorescence - Anti-PYK2 antibody [YE353] - BSA and Azide free (AB228477), expandable thumbnail](https://content.abcam.com/products/images/pyk2-antibody-ye353-bsa-and-azide-free-ab228477--immunocytochemistry-immunofluorescence-img68952.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-PYK2 antibody [YE353] - BSA and Azide free (AB228477)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 antibody [YE353] - BSA and Azide free (AB228477), expandable thumbnail](https://content.abcam.com/products/images/pyk2-antibody-ye353-bsa-and-azide-free-ab228477--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img30259.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 antibody [YE353] - BSA and Azide free (AB228477)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 antibody [YE353] - BSA and Azide free (AB228477), expandable thumbnail](https://content.abcam.com/products/images/pyk2-antibody-ye353-bsa-and-azide-free-ab228477--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img16860.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 antibody [YE353] - BSA and Azide free (AB228477)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 antibody [YE353] - BSA and Azide free (AB228477), expandable thumbnail](https://content.abcam.com/products/images/pyk2-antibody-ye353-bsa-and-azide-free-ab228477--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img106337.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 antibody [YE353] - BSA and Azide free (AB228477)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|
| Human | Not recommended | Tested | Tested | Tested |
| Mouse | Not recommended | Expected | Expected | Tested |
| Rat | Not recommended | Expected | Tested | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
7 products for Alternative Product
3 products for Alternative Version
Target data
Function
Non-receptor protein-tyrosine kinase that regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading and bone remodeling. Plays a role in the regulation of the humoral immune response, and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. Required for normal macrophage polarization and migration towards sites of inflammation. Regulates cytoskeleton rearrangement and cell spreading in T-cells, and contributes to the regulation of T-cell responses. Promotes osteoclastic bone resorption; this requires both PTK2B/PYK2 and SRC. May inhibit differentiation and activity of osteoprogenitor cells. Functions in signaling downstream of integrin and collagen receptors, immune receptors, G-protein coupled receptors (GPCR), cytokine, chemokine and growth factor receptors, and mediates responses to cellular stress. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Promotes activation of NOS3. Regulates production of the cellular messenger cGMP. Promotes activation of the MAP kinase signaling cascade, including activation of MAPK1/ERK2, MAPK3/ERK1 and MAPK8/JNK1. Promotes activation of Rho family GTPases, such as RHOA and RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Acts as a scaffold, binding to both PDPK1 and SRC, thereby allowing SRC to phosphorylate PDPK1 at 'Tyr-9, 'Tyr-373', and 'Tyr-376'. Promotes phosphorylation of NMDA receptors by SRC family members, and thereby contributes to the regulation of NMDA receptor ion channel activity and intracellular Ca(2+) levels. May also regulate potassium ion transport by phosphorylation of potassium channel subunits. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ASAP1, NPHP1, KCNA2 and SHC1. Promotes phosphorylation of ASAP2, RHOU and PXN; this requires both SRC and PTK2/PYK2.
Alternative names
FAK2, PYK2, RAFTK, PTK2B, Protein-tyrosine kinase 2-beta, Calcium-dependent tyrosine kinase, Calcium-regulated non-receptor proline-rich tyrosine kinase, Cell adhesion kinase beta, Focal adhesion kinase 2, Proline-rich tyrosine kinase 2, Related adhesion focal tyrosine kinase, CADTK, CAK-beta, CAKB, FADK 2
Recommended products
Rabbit Recombinant Monoclonal PYK2 antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Rat, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- YE353
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody recognizes PYK2. It does not cross react with other FAK family members.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab228477 is the carrier-free version of Anti-PYK2 antibody [YE353] ab32571.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PYK2 also known as Protein Tyrosine Kinase 2 Beta (PTK2B) is a protein with a mass of approximately 116 kDa. This enzyme belongs to the focal adhesion kinase (FAK) family and functions as a non-receptor protein tyrosine kinase. Mechanically PYK2 is involved in calcium-dependent signaling and is activated by increases in intracellular calcium levels. It plays a critical role in cell proliferation migration and survival. PYK2 expresses widely in neuronal tissues hematopoietic cells and various epithelial cells.
- Biological function summary
PYK2 interacts with integrins and G-protein-coupled receptors (GPCRs) linking extracellular signals to intracellular pathways. It forms complexes with other signaling molecules including Rac1 and c-Src facilitating downstream signaling. These interactions contribute to cellular communication and adhesion processes especially in the central nervous system and immune cells. Through its involvement in signal transduction PYK2 supports cellular responses to environmental changes.
- Pathways
PYK2 participates in the MAPK and JNK signaling pathways. These pathways are critical for transmitting signals from the cell surface to the nucleus influencing cellular growth and stress responses. PYK2 interacts closely with other proteins such as Src and FAK which help coordinate signals that regulate cellular dynamics. Through these pathways PYK2 modulates processes involved in the structural reorganization of cells and tissues.
- Associated diseases and disorders
PYK2 has been linked to neurodegenerative diseases and certain types of cancers. In Alzheimer's disease PYK2 may contribute to synaptic dysfunction and cognitive decline as it interacts with amyloid-beta peptides and tau proteins. In cancer PYK2 overexpression can promote cancer cell invasion metastasis and resistance to apoptosis often in coordination with proteins like FAK. Therefore understanding the role of PYK2 in these conditions provides insights for therapeutic developments.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Western blot - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477), expandable thumbnail](https://content.abcam.com/products/images/pyk2-antibody-ye353-bsa-and-azide-free-ab228477--western-blot-img51076.jpg "Western blot - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477)")
Western blot - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477)
This WB data was generated using the same anti-PYK2 antibody clone, YE353, in a different buffer formulation (cat# Anti-PYK2 antibody [YE353] ab32571).
Lane 1: Wild type HAP1 whole cell lysate (20 μg)
Lane 2: PYK2 knockout HAP1 whole cell lysate (20 μg)
Lane 3: Jurkat whole cell lysate (20 μg)
Lane 4: Hu brain whole cell lysate (20 μg)Lanes 1 - 4: Merged signal (red and green). Green - Anti-PYK2 antibody [YE353] ab32571 observed at 125 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
Anti-PYK2 antibody [YE353] ab32571 was shown to recognize PYK2 when PYK2 knockout samples were used, along with additional cross-reactive bands. Wild-type and PYK2 knockout samples were subjected to SDS-PAGE. Anti-PYK2 antibody [YE353] ab32571 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-PYK2 antibody [YE353] (Anti-PYK2 antibody [YE353] ab32571)
Predicted band size: 116 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477), expandable thumbnail](https://content.abcam.com/products/images/pyk2-antibody-ye353-bsa-and-azide-free-ab228477--immunocytochemistry-immunofluorescence-img68952.jpg "Immunocytochemistry/ Immunofluorescence - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477)")
Immunocytochemistry/ Immunofluorescence - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477)
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling PYK2 with purified Anti-PYK2 antibody [YE353] ab32571 at 1/60. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.
Control: primary antibody (1/100) and secondary antibody, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PYK2 antibody [YE353] ab32571).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477), expandable thumbnail](https://content.abcam.com/products/images/pyk2-antibody-ye353-bsa-and-azide-free-ab228477--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img30259.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebral cortex tissue labelling PYK2 with purified Anti-PYK2 antibody [YE353] ab32571 at 1/300. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PYK2 antibody [YE353] ab32571).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477), expandable thumbnail](https://content.abcam.com/products/images/pyk2-antibody-ye353-bsa-and-azide-free-ab228477--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img16860.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebral cortex tissue labelling PYK2 with purified Anti-PYK2 antibody [YE353] ab32571 at 1/300. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PYK2 antibody [YE353] ab32571).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477), expandable thumbnail](https://content.abcam.com/products/images/pyk2-antibody-ye353-bsa-and-azide-free-ab228477--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img106337.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue labelling PYK2 with purified Anti-PYK2 antibody [YE353] ab32571 at 1/300. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PYK2 antibody [YE353] ab32571).
![Immunocytochemistry/ Immunofluorescence - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477), expandable thumbnail](https://content.abcam.com/products/images/pyk2-antibody-ye353-bsa-and-azide-free-ab228477--immunocytochemistry-immunofluorescence-img89794.jpg "Immunocytochemistry/ Immunofluorescence - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477)")
Immunocytochemistry/ Immunofluorescence - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477)
Immunocytochemistry/Immunofluorescence analysis of PC12 cells labelling PYK2 with unpurified Anti-PYK2 antibody [YE353] ab32571 at a 1/100 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PYK2 antibody [YE353] ab32571).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477), expandable thumbnail](https://content.abcam.com/products/images/pyk2-antibody-ye353-bsa-and-azide-free-ab228477--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129863.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477)
This IHC data was generated using the same anti-PYK2 antibody clone, YE353, in a different buffer formulation (cat# Anti-PYK2 antibody [YE353] ab32571).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human brain tissue labelling PYK2 with unpurified Anti-PYK2 antibody [YE353] ab32571 at a 1/250 dilution.
![Western blot - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477), expandable thumbnail](https://content.abcam.com/products/images/pyk2-antibody-ye353-bsa-and-azide-free-ab228477--western-blot-img435355.jpg "Western blot - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477)")
Western blot - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477)
This data was developed using the same antibody clone in a different buffer formulation (abAB32571).
Western blot: Anti-PTK2B antibody [YE353] (Anti-PYK2 antibody [YE353] ab32571) staining at 1/2000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, Anti-PYK2 antibody [YE353] ab32571 was shown to bind specifically to PTK2B. A band was observed at 130 kDa in wild-type A549 cell lysates with no signal observed at this size in PTK2B knockout cell line. To generate this image, wild-type and PTK2B knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-PYK2 antibody [YE353] (Anti-PYK2 antibody [YE353] ab32571) at 1/2000 dilution
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: PTK2B knockout A549 cell lysate at 20 µg
Lane 3: Wild-type HAP1 cell lysate at 20 µg
Lane 4: PTK2B knockout HAP1 cell lysate at 20 µg
SecondaryAll lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 130 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477), expandable thumbnail](https://content.abcam.com/products/images/pyk2-antibody-ye353-bsa-and-azide-free-ab228477--immunocytochemistry-immunofluorescence-img89794.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477), expandable thumbnail](https://content.abcam.com/products/images/pyk2-antibody-ye353-bsa-and-azide-free-ab228477--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129863.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477)")
![Western blot - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477), expandable thumbnail](https://content.abcam.com/products/images/pyk2-antibody-ye353-bsa-and-azide-free-ab228477--western-blot-img435355.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477)")
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