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Rabbit Polyclonal PYK2 phospho Y402 antibody. Suitable for ICC/IF, WB, IHC-P and reacts with Mouse, Human samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human PTK2B phospho Y402.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human PTK2B phospho Y402. The exact immunogen used to generate this antibody is proprietary information. Database link Q14289
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBIHC-P
Human
Tested
Tested
Tested
Mouse
Tested
Expected
Tested

Tested
Tested

Species

Mouse

Dilution info

1.00000-2.00000 µg/mL

Notes

-

Species

Human

Dilution info

1.00000-2.00000 µg/mL

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/10.00000 - 1/50.00000

Notes

-

Species

Human

Dilution info

1/10.00000 - 1/50.00000

Notes

-

Target data

Function

Non-receptor protein-tyrosine kinase that regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading and bone remodeling. Plays a role in the regulation of the humoral immune response, and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. Required for normal macrophage polarization and migration towards sites of inflammation. Regulates cytoskeleton rearrangement and cell spreading in T-cells, and contributes to the regulation of T-cell responses. Promotes osteoclastic bone resorption; this requires both PTK2B/PYK2 and SRC. May inhibit differentiation and activity of osteoprogenitor cells. Functions in signaling downstream of integrin and collagen receptors, immune receptors, G-protein coupled receptors (GPCR), cytokine, chemokine and growth factor receptors, and mediates responses to cellular stress. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Promotes activation of NOS3. Regulates production of the cellular messenger cGMP. Promotes activation of the MAP kinase signaling cascade, including activation of MAPK1/ERK2, MAPK3/ERK1 and MAPK8/JNK1. Promotes activation of Rho family GTPases, such as RHOA and RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Acts as a scaffold, binding to both PDPK1 and SRC, thereby allowing SRC to phosphorylate PDPK1 at 'Tyr-9, 'Tyr-373', and 'Tyr-376'. Promotes phosphorylation of NMDA receptors by SRC family members, and thereby contributes to the regulation of NMDA receptor ion channel activity and intracellular Ca(2+) levels. May also regulate potassium ion transport by phosphorylation of potassium channel subunits. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ASAP1, NPHP1, KCNA2 and SHC1. Promotes phosphorylation of ASAP2, RHOU and PXN; this requires both SRC and PTK2/PYK2.

Alternative names

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Rabbit Polyclonal PYK2 phospho Y402 antibody. Suitable for ICC/IF, WB, IHC-P and reacts with Mouse, Human samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human PTK2B phospho Y402.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human PTK2B phospho Y402. The exact immunogen used to generate this antibody is proprietary information. Database link Q14289
Purification technique

Affinity purification Immunogen

Concentration
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Purification notes

Purified from rabbit serum by epitope specific chromatography. The antibody has been negatively pre-adsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PYK 2. The final product is generated by affinity chromatography using a PYK 2-derived peptide that is phosphorylated at tyrosine 402.

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

PYK2 interacts with integrins and G-protein-coupled receptors (GPCRs) linking extracellular signals to intracellular pathways. It forms complexes with other signaling molecules including Rac1 and c-Src facilitating downstream signaling. These interactions contribute to cellular communication and adhesion processes especially in the central nervous system and immune cells. Through its involvement in signal transduction PYK2 supports cellular responses to environmental changes.

Activity summary

PYK2 also known as Protein Tyrosine Kinase 2 Beta (PTK2B) is a protein with a mass of approximately 116 kDa. This enzyme belongs to the focal adhesion kinase (FAK) family and functions as a non-receptor protein tyrosine kinase. Mechanically PYK2 is involved in calcium-dependent signaling and is activated by increases in intracellular calcium levels. It plays a critical role in cell proliferation migration and survival. PYK2 expresses widely in neuronal tissues hematopoietic cells and various epithelial cells.

Pathways

PYK2 participates in the MAPK and JNK signaling pathways. These pathways are critical for transmitting signals from the cell surface to the nucleus influencing cellular growth and stress responses. PYK2 interacts closely with other proteins such as Src and FAK which help coordinate signals that regulate cellular dynamics. Through these pathways PYK2 modulates processes involved in the structural reorganization of cells and tissues.

Associated diseases and disorders

PYK2 has been linked to neurodegenerative diseases and certain types of cancers. In Alzheimer's disease PYK2 may contribute to synaptic dysfunction and cognitive decline as it interacts with amyloid-beta peptides and tau proteins. In cancer PYK2 overexpression can promote cancer cell invasion metastasis and resistance to apoptosis often in coordination with proteins like FAK. Therefore understanding the role of PYK2 in these conditions provides insights for therapeutic developments.

Product promise

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6 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-PYK2 (phospho Y402) antibody (ab4800), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PYK2 (phospho Y402) antibody (ab4800)

    A549 cells stained for PYK2 (green) using ab4800 at 1/500 dilution in ICC/IF. Followed by Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution for 45 minutes at room temperature (Panel a). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin at 1/300 dilution. Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 (phospho Y402) antibody (ab4800), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 (phospho Y402) antibody (ab4800)

    Paraffin embedded human hepatocarcinoma tissue (right) stained for PYK2 using ab4800 at 1/50 dilution in immunohistochemical analysis. Negative control (left) without primary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 (phospho Y402) antibody (ab4800), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 (phospho Y402) antibody (ab4800)

    Paraffin embedded Mouse brain tissue (right) stained for PYK2 using ab4800 at 1/20 dilution in immunohistochemical analysis. Negative control (left) without primary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 (phospho Y402) antibody (ab4800), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 (phospho Y402) antibody (ab4800)

    Paraffin embedded human lung adenocarcinoma tissue(right) stained for PYK2 using ab4800 at 1/20 dilution in immunohistochemical analysis. Negative control (left) without primary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 (phospho Y402) antibody (ab4800), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 (phospho Y402) antibody (ab4800)

    Paraffin embedded human brain tissue (right) stained for PYK2 using ab4800 at 1/50 dilution in immunohistochemical analysis. Negative control (left) without primary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-PYK2 (phospho Y402) antibody (ab4800), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PYK2 (phospho Y402) antibody (ab4800)

    Figure shows Pyk2 staining: Normal mouse mammary (NmuMG) cells were treated with TGFbeta and then grown to confluence, fixed and permeabilized. ab4800 was identified using a selective PSSA followed by incubation with a Cy3-conjugated anti-rabbit secondary antibody (red). F actin was visualized with Oregon Green-conjugated phalloiden (green) and paxillin staining is indicated in blue. The merged image indicates colocalization (purple) of ab4800, F-actin and paxillin in focal adhesion structures.

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Product protocols

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