Rabbit Recombinant Monoclonal Pyrin antibody. Suitable for IP, WB and reacts with Mouse samples. Cited in 14 publications.
Images
![Western blot - Anti-Pyrin antibody [EPR18676] (AB195975), expandable thumbnail](https://content.abcam.com/products/images/pyrin-antibody-epr18676-ab195975--western-blot-img25581.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Pyrin antibody [EPR18676] (AB195975)")
![Western blot - Anti-Pyrin antibody [EPR18676] (AB195975), expandable thumbnail](https://content.abcam.com/products/images/pyrin-antibody-epr18676-ab195975--western-blot-img101596.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Pyrin antibody [EPR18676] (AB195975)")
![Immunoprecipitation - Anti-Pyrin antibody [EPR18676] (AB195975), expandable thumbnail](https://content.abcam.com/products/images/pyrin-antibody-epr18676-ab195975--immunoprecipitation-img70168.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Pyrin antibody [EPR18676] (AB195975)")
Publications
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- mBio 14:e02066232023Phosphoprotein phosphatase activity positively regulates oligomeric pyrin to trigger inflammasome assembly in phagocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaleema S Malik,Flora Magnotti,Nicole A Loeven,Jose M Delgado,Arminja N Kettenbach,Thomas Henry,James B BliskaPubMed 37787552
- Frontiers in immunology 14:11466122023Alpha-linolenic acid pretreatment alleviates NETs-induced alveolar macrophage pyroptosis by inhibiting pyrin inflammasome activation in a mouse model of sepsis-induced ALI/ARDS.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChenchen Liu,Yu Zhou,Qing Tu,Liangfang Yao,Jinbao Li,Zhongwei YangPubMed 37051243
- Cell reports 41:1116882022Neutrophil inflammasomes sense the subcellular delivery route of translocated bacterial effectors and toxins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChanghoon Oh,Lupeng Li,Ambika Verma,Arianna D Reuven,Edward A Miao,James B Bliska,Youssef AachouiPubMed 36417874
- JACC. Basic to translational science 6:964-9802021Rivaroxaban, a Direct Oral Factor Xa Inhibitor, Attenuates Atherosclerosis by Alleviating Factor Xa-PAR2-Mediated Autophagy Suppression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYusuke Ito,Yasuhiro Maejima,Shun Nakagama,Yuka Shiheido-Watanabe,Natsuko Tamura,Tetsuo SasanoPubMed 35024502
- mBio 12:e02098212021The Burkholderia cenocepacia Type VI Secretion System Effector TecA Is a Virulence Factor in Mouse Models of Lung Infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNicole A Loeven,Andrew I Perault,Peggy A Cotter,Craig A Hodges,Joseph D Schwartzman,Thomas H Hampton,James B BliskaPubMed 34579569
- Nature 597:415-4192021AIM2 forms a complex with pyrin and ZBP1 to drive PANoptosis and host defence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSangJoon Lee,Rajendra Karki,Yaqiu Wang,Lam Nhat Nguyen,Ravi C Kalathur,Thirumala-Devi KannegantiPubMed 34471287
- Journal of immunology (Baltimore, Md. : 1950) 205:2778-27852020RIPK3 Promotes Expression and Pyrin Inflammasome Activation via Modulation of mTOR Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDeepika Sharma et. al.PubMed 32989095
- Methods in molecular biology (Clifton, N.J.) 2010:241-2552019Methods for Detection of Pyrin Inflammasome Assembly in Macrophages Infected with Yersinia spp.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNatasha P Medici,James B BliskaPubMed 31177443
- Infection and immunity 87:2019Characterization of Pyrin Dephosphorylation and Inflammasome Activation in Macrophages as Triggered by the Effectors YopE and YopT.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNatasha P Medici et. al.PubMed 30602502
- The Journal of clinical investigation 129:150-1622018TNF/TNFR axis promotes pyrin inflammasome activation and distinctly modulates pyrin inflammasomopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDeepika Sharma,Ankit Malik,Clifford Guy,Peter Vogel,Thirumala-Devi KannegantiPubMed 30457980
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Mouse | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Involved in the regulation of innate immunity and the inflammatory response in response to IFNG/IFN-gamma (PubMed:10807793, PubMed:11468188, PubMed:16037825, PubMed:16785446, PubMed:17431422, PubMed:17964261, PubMed:18577712, PubMed:19109554, PubMed:19584923, PubMed:26347139, PubMed:27030597, PubMed:28835462). Organizes autophagic machinery by serving as a platform for the assembly of ULK1, Beclin 1/BECN1, ATG16L1, and ATG8 family members and recognizes specific autophagy targets, thus coordinating target recognition with assembly of the autophagic apparatus and initiation of autophagy (PubMed:16785446, PubMed:17431422, PubMed:26347139). Acts as an autophagy receptor for the degradation of several inflammasome components, including CASP1, NLRP1 and NLRP3, hence preventing excessive IL1B- and IL18-mediated inflammation (PubMed:16785446, PubMed:17431422, PubMed:26347139). However, it can also have a positive effect in the inflammatory pathway, acting as an innate immune sensor that triggers PYCARD/ASC specks formation, caspase-1 activation, and IL1B and IL18 production (PubMed:16037825, PubMed:27030597, PubMed:28835462). Together with AIM2, also acts as a mediator of pyroptosis, necroptosis and apoptosis (PANoptosis), an integral part of host defense against pathogens, in response to bacterial infection (By similarity). It is required for PSTPIP1-induced PYCARD/ASC oligomerization and inflammasome formation (PubMed:10807793, PubMed:11468188, PubMed:17964261, PubMed:18577712, PubMed:19109554, PubMed:19584923). Recruits PSTPIP1 to inflammasomes, and is required for PSTPIP1 oligomerization (PubMed:10807793, PubMed:11468188, PubMed:17964261, PubMed:18577712, PubMed:19109554, PubMed:19584923).
Alternative names
MEF, TRIM20, MEFV, Pyrin, Marenostrin
Recommended products
Rabbit Recombinant Monoclonal Pyrin antibody. Suitable for IP, WB and reacts with Mouse samples. Cited in 14 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR18676
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Pyrin also known as marenostrin or MEFV is a protein involved in the innate immune response. It has a molecular mass of about 88 kDa. Pyrin gets mainly expressed in neutrophils and macrophages cells integral to the body's defensive mechanisms. Mechanically this protein plays a role in modulating the assembly of inflammasomes which are multiprotein complexes essential for the production of pro-inflammatory cytokines such as interleukin-1 beta.
- Biological function summary
Pyrin functions by interacting with the cytosolic adapter protein ASC forming part of the pyrin inflammasome complex. This interaction initiates an inflammatory response. The pyrin-meditated inflammasome assembly is important in regulating the body's response to pathogenic microorganisms and cellular stress. In addition to its immune role pyrin may regulate apoptosis the process of programmed cell death under certain conditions.
- Pathways
The pyrin protein is a critical component of the inflammasome signaling pathway and the interleukin-1 signaling pathway. Within these pathways pyrin interacts directly with proteins like caspase-1 which processes pro-inflammatory cytokines into their active forms. These pathways play significant roles in various immune responses and help maintain homeostasis by modulating inflammation and cell death processes.
- Associated diseases and disorders
Pyrin mutations are linked to Familial Mediterranean Fever (FMF) and Behçet's disease. FMF a hereditary inflammatory disorder results from pyrin's inability to properly regulate inflammasome activity causing recurrent fever abdominal pain and inflammation in joints and skin. Additionally Behçet's disease characterized by recurrent oral and genital ulcers is associated with abnormal pyrin functioning. Changes in the pyrin protein may also interact with proteins like NLRP3 influencing susceptibility to these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Pyrin antibody [EPR18676] (ab195975), expandable thumbnail](https://content.abcam.com/products/images/pyrin-antibody-epr18676-ab195975--western-blot-img25581.jpg "Western blot - Anti-Pyrin antibody [EPR18676] (ab195975)")
Western blot - Anti-Pyrin antibody [EPR18676] (ab195975)
Blocking/Dilution buffer: 5% milk/TBST.
The image was kindly provided by our collaborator Dr Feng Shao's lab, NIBS.
All lanes: Western blot - Anti-Pyrin antibody [EPR18676] (ab195975) at 1/1000 dilution
Lane 1: Bone marrow derived-macrophage of Pyrin -/- mice at 5 µg
Lane 2: Bone marrow derived-macrophage of Pyrin -/- mice stimulated with LPS at 5 µg
Lane 3: Bone marrow derived-macrophage of Pyrin -/- mice stimulated with TNF alpha at 5 µg
Lane 4: Bone marrow derived-macrophage of wild type C57/B6 mice at 5 µg
Lane 5: Bone marrow derived-macrophage of wild type C57/B6 mice stimulated with LPS at 5 µg
Lane 6: Bone marrow derived-macrophage of wild type C57/B6 mice stimulated with TNF alpha at 5 µg
SecondaryAll lanes: Goat anti-Rabbit IgG (H+L) at 1/5000 dilution
Predicted band size: 86 kDa
Observed band size: 110 kDa
Exposure time: 60s
![Western blot - Anti-Pyrin antibody [EPR18676] (ab195975), expandable thumbnail](https://content.abcam.com/products/images/pyrin-antibody-epr18676-ab195975--western-blot-img101596.jpg "Western blot - Anti-Pyrin antibody [EPR18676] (ab195975)")
Western blot - Anti-Pyrin antibody [EPR18676] (ab195975)
Blocking/Dilution buffer: 5% NFDM/TBST.
The lysates were kindly provided by our collaborator Dr Feng Shao's lab, NIBS.
All lanes: Western blot - Anti-Pyrin antibody [EPR18676] (ab195975) at 1/50 dilution
Lane 1: Bone marrow derived-macrophage Pyrin -/- mice at 10 µg
Lane 2: Bone marrow derived-macrophage Pyrin -/- mice stimulated with LPS at 10 µg
Lane 3: Bone marrow derived-macrophage of wild type C57/B6 mice at 10 µg
Lane 4: Bone marrow derived-macrophage of wild type C57/B6 mice stimulated with LPS at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/2000 dilution
Predicted band size: 86 kDa
Exposure time: 30s
![Immunoprecipitation - Anti-Pyrin antibody [EPR18676] (ab195975), expandable thumbnail](https://content.abcam.com/products/images/pyrin-antibody-epr18676-ab195975--immunoprecipitation-img70168.jpg "Immunoprecipitation - Anti-Pyrin antibody [EPR18676] (ab195975)")
Immunoprecipitation - Anti-Pyrin antibody [EPR18676] (ab195975)
Pyrin was immunoprecipitated from DC2.4 (Human immature dendritic cell line) stable Pyrin expression whole cell lysate with ab195975 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab195975 at 1/1000 dilution. Goat anti-Rabbit IgG (H+L), was used as secondary antibody at 1/5000 dilution.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
The image was kindly provided by Dr Feng Shao's lab, NIBS.
All lanes: Immunoprecipitation - Anti-Pyrin antibody [EPR18676] (ab195975) at 1/100 dilution
Lane 1: DC2.4 whole cell lysate
Lanes 2 - 3: DC2.4 stable Pyrin expression whole cell lysate
Lane 4: ab195975 IP in DC2.4 whole cell lysate
Lane 5: ab195975 IP in DC2.4 stable Pyrin expression whole cell lysate
Lane 6: Mock IP (without ab195975) in DC2.4 stable Pyrin expression whole cell lysate
Predicted band size: 86 kDa
Exposure time: 30s
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