AB200420

Anti-Pyrin (phospho S241) antibody [EPR19570]

RabMAb
Recombinant
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(10 Publications)

Rabbit Recombinant Monoclonal Pyrin phospho S241 antibody. Suitable for Dot, WB and reacts with Synthetic peptide, Transfected cell lysate - Mouse samples. Cited in 10 publications.

View Alternative Names

Pyrin, Mefv

1 Images

Supplier Data

Dot Blot - Anti-Pyrin (phospho S241) antibody [EPR19570] (AB200420)

Dot blot analysis of Pyrin (phospho S241) labeled with ab200420 at 1/1000 dilution.

Lane 1 : Pyrin (phospho S241) phospho peptide;

Lane 2 : Pyrin Non-phospho peptide.

Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

Carrier free

Anti-Pyrin (phospho S241) antibody [EPR19570] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19570

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Synthetic peptide": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - Mouse": { "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pyrin also known as marenostrin or MEFV is a protein involved in the innate immune response. It has a molecular mass of about 88 kDa. Pyrin gets mainly expressed in neutrophils and macrophages cells integral to the body's defensive mechanisms. Mechanically this protein plays a role in modulating the assembly of inflammasomes which are multiprotein complexes essential for the production of pro-inflammatory cytokines such as interleukin-1 beta.

Biological function summary

Pyrin functions by interacting with the cytosolic adapter protein ASC forming part of the pyrin inflammasome complex. This interaction initiates an inflammatory response. The pyrin-meditated inflammasome assembly is important in regulating the body's response to pathogenic microorganisms and cellular stress. In addition to its immune role pyrin may regulate apoptosis the process of programmed cell death under certain conditions.

Pathways

The pyrin protein is a critical component of the inflammasome signaling pathway and the interleukin-1 signaling pathway. Within these pathways pyrin interacts directly with proteins like caspase-1 which processes pro-inflammatory cytokines into their active forms. These pathways play significant roles in various immune responses and help maintain homeostasis by modulating inflammation and cell death processes.

Pyrin mutations are linked to Familial Mediterranean Fever (FMF) and Behçet's disease. FMF a hereditary inflammatory disorder results from pyrin's inability to properly regulate inflammasome activity causing recurrent fever abdominal pain and inflammation in joints and skin. Additionally Behçet's disease characterized by recurrent oral and genital ulcers is associated with abnormal pyrin functioning. Changes in the pyrin protein may also interact with proteins like NLRP3 influencing susceptibility to these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the regulation of innate immunity and the inflammatory response in response to IFNG/IFN-gamma (PubMed : 34471287). Organizes autophagic machinery by serving as a platform for the assembly of ULK1, Beclin 1/BECN1, ATG16L1, and ATG8 family members and recognizes specific autophagy targets, thus coordinating target recognition with assembly of the autophagic apparatus and initiation of autophagy (By similarity). Acts as an autophagy receptor for the degradation of several inflammasome components, including CASP1, NLRP1 and NLRP3, hence preventing excessive IL1B- and IL18-mediated inflammation (By similarity). However, it can also have a positive effect in the inflammatory pathway, acting as an innate immune sensor that triggers PYCARD/ASC specks formation, caspase-1 activation, and IL1B and IL18 production (By similarity). Together with AIM2, also acts as a mediator of pyroptosis, necroptosis and apoptosis (PANoptosis), an integral part of host defense against pathogens, in response to bacterial infection (PubMed : 34471287). It is required for PSTPIP1-induced PYCARD/ASC oligomerization and inflammasome formation (By similarity). Recruits PSTPIP1 to inflammasomes, and is required for PSTPIP1 oligomerization (By similarity).

See full target information Mefv phospho S241

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Investigative ophthalmology & visual science 66:33 PubMed39808118

2025

Piezo1 Enhances Macrophage Phagocytosis and Pyrin Activation to Ameliorate Fungal Keratitis.

Applications

Unspecified application

Species

Unspecified reactive species

Jiahui Yang,Jing Zhong,Zhenyuan Fu,Dalian He,Jing Zhang,Jin Yuan

Cell death & disease 14:213 PubMed36966139

2023

Mutations in the B30.2 and the central helical scaffold domains of pyrin differentially affect inflammasome activation.

Applications

Unspecified application

Species

Unspecified reactive species

Daria Chirita,Pauline Bronnec,Flora Magnotti,Sarah Dalmon,Amandine Martin,Michel Popoff,Mathieu Gerfaud-Valentin,Pascal Sève,Alexandre Belot,Anne Contis,Agnes Duquesne,Gaetane Nocturne,Irene Lemelle,Sophie Georgin-Lavialle,Guilaine Boursier,Isabelle Touitou,Yvan Jamilloux,Thomas Henry

PLoS biology 20:e3001351 PubMed36342970

2022

Transcriptional licensing is required for Pyrin inflammasome activation in human macrophages and bypassed by mutations causing familial Mediterranean fever.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew S J Mangan,Friederike Gorki,Karoline Krause,Alexander Heinz,Anne Pankow,Thomas Ebert,Dieter Jahn,Karsten Hiller,Veit Hornung,Marcus Maurer,Florian I Schmidt,Ralf Gerhard,Eicke Latz

Cell reports 41:111472 PubMed36223753

2022

Steroid hormone catabolites activate the pyrin inflammasome through a non-canonical mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Flora Magnotti,Daria Chirita,Sarah Dalmon,Amandine Martin,Pauline Bronnec,Jeremy Sousa,Olivier Helynck,Wonyong Lee,Daniel L Kastner,Jae Jin Chae,Michael F McDermott,Alexandre Belot,Michel Popoff,Pascal Sève,Sophie Georgin-Lavialle,Hélène Munier-Lehmann,Tu Anh Tran,Ellen De Langhe,Carine Wouters,Yvan Jamilloux,Thomas Henry

Methods in molecular biology (Clifton, N.J.) 2523:179-195 PubMed35759198

2022

Functional Assessment of Disease-Associated Pyrin Variants.

Applications

Unspecified application

Species

Unspecified reactive species

Daria Chirita,Yvan Jamilloux,Thomas Henry,Flora Magnotti

Journal of immunology (Baltimore, Md. : 1950) 205:2778-2785 PubMed32989095

2020

RIPK3 Promotes Expression and Pyrin Inflammasome Activation via Modulation of mTOR Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Deepika Sharma,Ankit Malik,Arjun Balakrishnan,R K Subbarao Malireddi,Thirumala-Devi Kanneganti

Nature immunology 21:857-867 PubMed32601469

2020

Ancient familial Mediterranean fever mutations in human pyrin and resistance to Yersinia pestis.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Hwan Park,Elaine F Remmers,Wonyong Lee,Amanda K Ombrello,Lawton K Chung,Zhao Shilei,Deborah L Stone,Maya I Ivanov,Nicole A Loeven,Karyl S Barron,Patrycja Hoffmann,Michele Nehrebecky,Yeliz Z Akkaya-Ulum,Erdal Sag,Banu Balci-Peynircioglu,Ivona Aksentijevich,Ahmet Gül,Charles N Rotimi,Hua Chen,James B Bliska,Seza Ozen,Daniel L Kastner,Daniel Shriner,Jae Jin Chae

EMBO molecular medicine 11:e10547 PubMed31589380

2019

Pyrin dephosphorylation is sufficient to trigger inflammasome activation in familial Mediterranean fever patients.

Applications

Unspecified application

Species

Unspecified reactive species

Flora Magnotti,Lucie Lefeuvre,Sarah Benezech,Tiphaine Malsot,Louis Waeckel,Amandine Martin,Sébastien Kerever,Daria Chirita,Marine Desjonqueres,Agnès Duquesne,Mathieu Gerfaud-Valentin,Audrey Laurent,Pascal Sève,Michel-Robert Popoff,Thierry Walzer,Alexandre Belot,Yvan Jamilloux,Thomas Henry

Proceedings of the National Academy of Sciences of 113:14384-14389 PubMed27911804

2016

Familial Mediterranean fever mutations lift the obligatory requirement for microtubules in Pyrin inflammasome activation.

Applications

Unspecified application

Species

Unspecified reactive species

Hanne Van Gorp,Pedro H V Saavedra,Nathalia M de Vasconcelos,Nina Van Opdenbosch,Lieselotte Vande Walle,Magdalena Matusiak,Giusi Prencipe,Antonella Insalaco,Filip Van Hauwermeiren,Dieter Demon,Delfien J Bogaert,Melissa Dullaers,Elfride De Baere,Tino Hochepied,Joke Dehoorne,Karim Y Vermaelen,Filomeen Haerynck,Fabrizio De Benedetti,Mohamed Lamkanfi

Proceedings of the National Academy of Sciences of 113:E4857-66 PubMed27482109

2016

Site-specific phosphorylation and microtubule dynamics control Pyrin inflammasome activation.

Applications

Unspecified application

Species

Unspecified reactive species

Wenqing Gao,Jieling Yang,Wang Liu,Yupeng Wang,Feng Shao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Pyrin (phospho S241) antibody [EPR19570]

Dot Blot - Anti-Pyrin (phospho S241) antibody [EPR19570] (AB200420)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

Product promise