Rabbit Recombinant Monoclonal Pyrin phospho S241 antibody. Suitable for Dot, WB and reacts with Synthetic peptide, Transfected cell lysate - Mouse samples. Cited in 7 publications.
Images
![Dot Blot - Anti-Pyrin (phospho S241) antibody [EPR19570] (AB200420), expandable thumbnail](https://content.abcam.com/products/images/pyrin-phospho-s241-antibody-epr19570-ab200420--dot-blot-img6141.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-Pyrin (phospho S241) antibody [EPR19570] (AB200420)")
Publications
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- Cell death & disease 14:2132023Mutations in the B30.2 and the central helical scaffold domains of pyrin differentially affect inflammasome activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaria Chirita,Pauline Bronnec,Flora Magnotti,Sarah Dalmon,Amandine Martin,Michel Popoff,Mathieu Gerfaud-Valentin,Pascal Sève,Alexandre Belot,Anne Contis,Agnes Duquesne,Gaetane Nocturne,Irene Lemelle,Sophie Georgin-Lavialle,Guilaine Boursier,Isabelle Touitou,Yvan Jamilloux,Thomas HenryPubMed 36966139
- PLoS biology 20:e30013512022Transcriptional licensing is required for Pyrin inflammasome activation in human macrophages and bypassed by mutations causing familial Mediterranean fever.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatthew S J Mangan,Friederike Gorki,Karoline Krause,Alexander Heinz,Anne Pankow,Thomas Ebert,Dieter Jahn,Karsten Hiller,Veit Hornung,Marcus Maurer,Florian I Schmidt,Ralf Gerhard,Eicke LatzPubMed 36342970
- Journal of immunology (Baltimore, Md. : 1950) 205:2778-27852020RIPK3 Promotes Expression and Pyrin Inflammasome Activation via Modulation of mTOR Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDeepika Sharma et. al.PubMed 32989095
- Nature immunology 21:857-8672020Ancient familial Mediterranean fever mutations in human pyrin and resistance to Yersinia pestis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYong Hwan Park,Elaine F Remmers,Wonyong Lee,Amanda K Ombrello,Lawton K Chung,Zhao Shilei,Deborah L Stone,Maya I Ivanov,Nicole A Loeven,Karyl S Barron,Patrycja Hoffmann,Michele Nehrebecky,Yeliz Z Akkaya-Ulum,Erdal Sag,Banu Balci-Peynircioglu,Ivona Aksentijevich,Ahmet Gül,Charles N Rotimi,Hua Chen,James B Bliska,Seza Ozen,Daniel L Kastner,Daniel Shriner,Jae Jin ChaePubMed 32601469
- EMBO molecular medicine 11:e105472019Pyrin dephosphorylation is sufficient to trigger inflammasome activation in familial Mediterranean fever patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFlora Magnotti et. al.PubMed 31589380
- Proceedings of the National Academy of Sciences of 113:14384-143892016Familial Mediterranean fever mutations lift the obligatory requirement for microtubules in Pyrin inflammasome activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHanne Van Gorp et. al.PubMed 27911804
- Proceedings of the National Academy of Sciences of 113:E4857-662016Site-specific phosphorylation and microtubule dynamics control Pyrin inflammasome activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenqing Gao et. al.PubMed 27482109
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Dot | WB | |
|---|---|---|
| Mouse | Predicted | Predicted |
| Synthetic peptide | Tested | Not recommended |
| Transfected cell lysate - Mouse | Not recommended | Tested |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Mouse | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Involved in the regulation of innate immunity and the inflammatory response in response to IFNG/IFN-gamma (PubMed:34471287). Organizes autophagic machinery by serving as a platform for the assembly of ULK1, Beclin 1/BECN1, ATG16L1, and ATG8 family members and recognizes specific autophagy targets, thus coordinating target recognition with assembly of the autophagic apparatus and initiation of autophagy (By similarity). Acts as an autophagy receptor for the degradation of several inflammasome components, including CASP1, NLRP1 and NLRP3, hence preventing excessive IL1B- and IL18-mediated inflammation (By similarity). However, it can also have a positive effect in the inflammatory pathway, acting as an innate immune sensor that triggers PYCARD/ASC specks formation, caspase-1 activation, and IL1B and IL18 production (By similarity). Together with AIM2, also acts as a mediator of pyroptosis, necroptosis and apoptosis (PANoptosis), an integral part of host defense against pathogens, in response to bacterial infection (PubMed:34471287). It is required for PSTPIP1-induced PYCARD/ASC oligomerization and inflammasome formation (By similarity). Recruits PSTPIP1 to inflammasomes, and is required for PSTPIP1 oligomerization (By similarity).
Alternative names
Pyrin, Mefv
Recommended products
Rabbit Recombinant Monoclonal Pyrin phospho S241 antibody. Suitable for Dot, WB and reacts with Synthetic peptide, Transfected cell lysate - Mouse samples. Cited in 7 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR19570
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Pyrin also known as marenostrin or MEFV is a protein involved in the innate immune response. It has a molecular mass of about 88 kDa. Pyrin gets mainly expressed in neutrophils and macrophages cells integral to the body's defensive mechanisms. Mechanically this protein plays a role in modulating the assembly of inflammasomes which are multiprotein complexes essential for the production of pro-inflammatory cytokines such as interleukin-1 beta.
- Biological function summary
Pyrin functions by interacting with the cytosolic adapter protein ASC forming part of the pyrin inflammasome complex. This interaction initiates an inflammatory response. The pyrin-meditated inflammasome assembly is important in regulating the body's response to pathogenic microorganisms and cellular stress. In addition to its immune role pyrin may regulate apoptosis the process of programmed cell death under certain conditions.
- Pathways
The pyrin protein is a critical component of the inflammasome signaling pathway and the interleukin-1 signaling pathway. Within these pathways pyrin interacts directly with proteins like caspase-1 which processes pro-inflammatory cytokines into their active forms. These pathways play significant roles in various immune responses and help maintain homeostasis by modulating inflammation and cell death processes.
- Associated diseases and disorders
Pyrin mutations are linked to Familial Mediterranean Fever (FMF) and Behçet's disease. FMF a hereditary inflammatory disorder results from pyrin's inability to properly regulate inflammasome activity causing recurrent fever abdominal pain and inflammation in joints and skin. Additionally Behçet's disease characterized by recurrent oral and genital ulcers is associated with abnormal pyrin functioning. Changes in the pyrin protein may also interact with proteins like NLRP3 influencing susceptibility to these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Dot Blot - Anti-Pyrin (phospho S241) antibody [EPR19570] (ab200420), expandable thumbnail](https://content.abcam.com/products/images/pyrin-phospho-s241-antibody-epr19570-ab200420--dot-blot-img6141.jpg "Dot Blot - Anti-Pyrin (phospho S241) antibody [EPR19570] (ab200420)")
Dot Blot - Anti-Pyrin (phospho S241) antibody [EPR19570] (ab200420)
Dot blot analysis of Pyrin (phospho S241) labeled with ab200420 at 1/1000 dilution.
Lane 1: Pyrin (phospho S241) phospho peptide;
Lane 2: Pyrin Non-phospho peptide.
Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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