AB96099

Anti-Pyrophosphatase 1 antibody

Name: Anti-Pyrophosphatase 1 antibody (ab96099) | Abcam
Brand: Abcam Limited
SKU: AB96099
Price: 520 USD
Availability: InStock

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(6 Publications)

Rabbit Polyclonal Pyrophosphatase 1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PPA1 aa 1 to C-terminus.

View Alternative Names

IOPPP, PP, PPA1, Inorganic pyrophosphatase, Pyrophosphate phospho-hydrolase, PPase

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-Pyrophosphatase 1 antibody (AB96099)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Pyrophosphatase 1 antibody (AB96099)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab96099 at 1/200 dilution. Lower image merged with DNA probe.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pyrophosphatase 1 antibody (AB96099)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pyrophosphatase 1 antibody (AB96099)

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using ab96099 at 1/500 dilution.

Supplier Data

Western blot - Anti-Pyrophosphatase 1 antibody (AB96099)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-Pyrophosphatase 1 antibody (ab96099) at 1/10000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

A431 whole cell lysates at 30 µg

Lane 3:

HeLa whole cell lysates at 30 µg

Lane 4:

HepG2 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 33 kDa

false

Supplier Data

Western blot - Anti-Pyrophosphatase 1 antibody (AB96099)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-Pyrophosphatase 1 antibody (ab96099) at 1/1000 dilution

Lane 1:

Neuro2A whole cell lysates at 30 µg

Lane 2:

GL261 whole cell lysates at 30 µg

Lane 3:

C8D30 whole cell lysates at 30 µg

Lane 4:

NIH-3T3 whole cell lysates at 30 µg

Lane 5:

BCL-1 whole cell lysates at 30 µg

Lane 6:

Raw 264.7 whole cell lysates at 30 µg

Lane 7:

C2Cl2 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 33 kDa

false

Supplier Data

Western blot - Anti-Pyrophosphatase 1 antibody (AB96099)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-Pyrophosphatase 1 antibody (ab96099) at 1/1000 dilution

Lane 1:

PC-12 whole cell lysates at 30 µg

Lane 2:

Rat2 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 33 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human PPA1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q15181

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pyrophosphatase 1 also known as iPPase or inorganic pyrophosphatase 1 is an enzyme responsible for the hydrolysis of inorganic pyrophosphate (PPi) into two phosphate ions. This reaction is energetically favorable and provides the driving force for many biosynthetic processes. Pyrophosphatase 1 is a soluble cytosolic enzyme with a molecular mass of about 33 kDa. It is expressed in various tissues especially high in metabolically active cells like those found in the liver and kidney.

Biological function summary

Pyrophosphate hydrolysis by pyrophosphatase 1 plays a critical role in energy metabolism and phosphate recycling within cells. This enzyme functions independently and is not part of a larger complex. Its activity maintains the balance of phosphate compounds in the cytosol. This balance is important for cellular functions like DNA and RNA synthesis where the removal of PPi is necessary to drive these reactions forward.

Pathways

Pyrophosphatase 1 influences several key metabolic routes such as nucleotide and protein biosynthesis. The enzyme ensures the continuation of these pathways by keeping PPi concentrations low which is important for reactions like aminoacyl-tRNA synthetase activity. Pyrophosphatase 1 connects with enzymes like polymerases and ligases through these pathways facilitating a range of anabolic processes by removing inhibitory PPi byproducts.

The dysregulation of pyrophosphatase 1 can impact conditions like hypophosphatasia and some forms of arthritis. In hypophosphatasia defective function of alkaline phosphatase leads to inadequate pyrophosphate breakdown affecting bone mineralization. Pyrophosphatase 1 by regulating PPi indirectly influences the symptoms when phosphate balance is disrupted. Furthermore its role in phosphate metabolism associates pyrophosphatase 1 with pathways involving matrix proteins that are implicated in mineralization disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information PPA1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Contrast media & molecular imaging 2022:1032557 PubMed36072623

2022

Circ TYMP1 Inhibits Carcinogenesis and Cisplatin Resistance in Ovarian Cancer by Reducing Smad2/3 Phosphorylation via a MicroRNA-182A-3p/TGF1B Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Rao,Wenwen Zhang,Dan Li,Xiao Li,Yaomei Ma,Pengpeng Qu

The Tohoku journal of experimental medicine 241:165-173 PubMed28202851

2017

Expression of Inorganic Pyrophosphatase (PPA1) Correlates with Poor Prognosis of Epithelial Ovarian Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Li,Ning Xiao,Zongpeng Li,Qin Wang

JPEN. Journal of parenteral and enteral nutrition 41:1293-1300 PubMed27503937

2016

Glucagon-Like Peptide 2 Stimulates Postresection Intestinal Adaptation in Preterm Pigs by Affecting Proteins Related to Protein, Carbohydrate, and Sulphur Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Pingping Jiang,Andreas Vegge,Thomas Thymann,Jennifer Man-Fan Wan,Per Torp Sangild

Molecular & cellular proteomics : MCP 12:2006-20 PubMed23462207

2013

Proteomic differences between hepatocellular carcinoma and nontumorous liver tissue investigated by a combined gel-based and label-free quantitative proteomics study.

Applications

Unspecified application

Species

Unspecified reactive species

Dominik A Megger,Thilo Bracht,Michael Kohl,Maike Ahrens,Wael Naboulsi,Frank Weber,Andreas-Claudius Hoffmann,Christian Stephan,Katja Kuhlmann,Martin Eisenacher,Jörg F Schlaak,Hideo A Baba,Helmut E Meyer,Barbara Sitek

PloS one 7:e44929 PubMed23028687

2012

Antibiotics increase gut metabolism and antioxidant proteins and decrease acute phase response and necrotizing enterocolitis in preterm neonates.

Applications

Species

Unspecified reactive species

Pingping Jiang,Michael Ladegaard Jensen,Malene Skovsted Cilieborg,Thomas Thymann,Jennifer Man-Fan Wan,Wai-Hung Sit,George L Tipoe,Per Torp Sangild

PloS one 7:e34524 PubMed22485177

2012

Identification of the inorganic pyrophosphate metabolizing, ATP substituting pathway in mammalian spermatozoa.

Applications

WB, ICC/IF

Species

Pig, Pig

Young-Joo Yi,Miriam Sutovsky,Chelsey Kennedy,Peter Sutovsky

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Pyrophosphatase 1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Pyrophosphatase 1 antibody (AB96099)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pyrophosphatase 1 antibody (AB96099)

Western blot - Anti-Pyrophosphatase 1 antibody (AB96099)

Western blot - Anti-Pyrophosphatase 1 antibody (AB96099)

Western blot - Anti-Pyrophosphatase 1 antibody (AB96099)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Primary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise