Anti-Pyruvate Dehydrogenase E2 antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Pyruvate Dehydrogenase E2 antibody (AB126224)

Immunofluorescence analysis of HeLa cells fixed in ice-cold MeOH for 5 min labelling DLAT protein at mitochondria using ab126224 at a 1/500 dilution (Green). Hoechst 33342 was used for nuclear staining (Blue).

• WB

Supplier Data

Western blot - Anti-Pyruvate Dehydrogenase E2 antibody (AB126224)

Samples were separated by 7.5% SDS PAGE.

All lanes:

Western blot - Anti-Pyruvate Dehydrogenase E2 antibody (ab126224) at 1/1000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

A431 whole cell lysates at 30 µg

Lane 3:

HeLa whole cell lysates at 30 µg

Lane 4:

HepG2 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 69 kDa

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• WB

CiteAb

Western blot - Anti-Pyruvate Dehydrogenase E2 antibody (AB126224)

Pyruvate Dehydrogenase E2 western blot using anti-Pyruvate Dehydrogenase E2 antibody ab126224. Publication image and figure legend from Osinalde, N., Sánchez-Quiles, V., et al., 2016, Sci Rep, PubMed 27025927.

ab126224 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab126224 please see the product overview.

IL-2-inducible Gab2-interacting proteins.(A) Heat map and hierarchical clustering dendrogram indicating the SILAC IL-2/Ctr ratio displayed by the cytokine-inducible Gab2-binding protein in each replica. (B) Validation of selected well-known and novel Gab2-binding proteins by immunoblot. (C) Relative binding abundance of the detected Gab2 interactors. *indicates described Gab2 interactors.

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