Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)]
- BOND RX™ Validated
- RabMAb
- Recombinant
- What is this?
4
(2 Reviews)
|
(14 Publications)
Rabbit Recombinant Monoclonal Pyruvate Dehydrogenase E2 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat, Transfected cell lysate - Mouse samples. Cited in 14 publications.
View Alternative Names
DLTA, DLAT, 70 kDa mitochondrial autoantigen of primary biliary cirrhosis, Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex, M2 antigen complex 70 kDa subunit, Pyruvate dehydrogenase complex component E2, PBC, PDC-E2, PDCE2
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] (AB172617)
Immunohistochemical analysis of paraffin-embedded human cervical cancer labeling Pyruvate Dehydrogenase E2 with ab172617 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Positive staining on human cervical cancer.
The section was incubated with ab172617 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
- Flow Cyt (Intra)
Supplier Data
Flow Cytometry (Intracellular) - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] (AB172617)
Intracellular flow cytometric analysis of permeabilized Jurkat cells labeling Pyruvate Dehydrogenase E2 with ab172617 at 1/10 dilution (red), compared to a rabbit IgG negative control (green).
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] (AB172617)
Immunofluorescent analysis of HepG2 cells labeling Pyruvate Dehydrogenase E2 with ab172617 at 1/50 dilution.
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] (AB172617)
Immunohistochemical analysis of paraffin-embedded human cerebrum labeling Pyruvate Dehydrogenase E2 with ab172617 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Positive staining on human cerebrum.
The section was incubated with ab172617 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
- IP
Supplier Data
Immunoprecipitation - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] (AB172617)
Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate immunoprecipitated using ab172617 at 1/10 dilution.
All lanes:
Immunoprecipitation - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] (ab172617)
Predicted band size: 69 kDa
false
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] (AB172617)
Immunohistochemical analysis of paraffin-embedded rat kidney labeling Pyruvate Dehydrogenase E2 with ab172617 at 1/500 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Positive staining on rat kidney.
The section was incubated with ab172617 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] (AB172617)
Immunohistochemical analysis of paraffin-embedded mouse kidney labeling Pyruvate Dehydrogenase E2 with ab172617 at 1/500 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Positive staining on mouse kidney.
The section was incubated with ab172617 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
- WB
Lab
Western blot - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] (AB172617)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
All lanes:
Western blot - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] (ab172617) at 1/1000 dilution
Lane 1:
Rat brain tissue lysate at 20 µg
Lane 2:
Rat kidney tissue lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 69 kDa
false
Exposure time: 15s
- WB
Lab
Western blot - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] (AB172617)
All lanes:
Western blot - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] (ab172617) at 1/1000 dilution
Lane 1:
C6 (rat glial tumor glial cell) whole cell lysate at 20 µg
Lane 2:
RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
Lane 3:
PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg
Lane 4:
NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 69 kDa
false
Exposure time: 48s
- WB
Lab
Western blot - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] (AB172617)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
All lanes:
Western blot - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] (ab172617) at 1/1000 dilution
Lane 1:
Mouse brain tissue lysate at 20 µg
Lane 2:
Mouse kidney tissue lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 69 kDa
false
Exposure time: 26s
- WB
Lab
Western blot - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] (AB172617)
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
All lanes:
Western blot - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] (ab172617) at 1/1000 dilution
Lane 1:
NIH/3T3 (mouse embryonic fibroblast) transfected with scrambled siRNA control whole cell lysate at 20 µg
Lane 2:
NIH/3T3 transfected with siRNA specifically targeting Dlat-1 whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Observed band size: 69 kDa
false
Exposure time: 15s
- WB
Supplier Data
Western blot - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] (AB172617)
All lanes:
Western blot - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] (ab172617) at 1/1000 dilution
Lane 1:
HeLa cell lysate at 10 µg
Lane 2:
HepG2 cell lysate at 10 µg
Lane 3:
Jurkat cell lysate at 10 µg
Lane 4:
293T cell lysate at 10 µg
Predicted band size: 69 kDa
false
Related conjugates and formulations (5)
-
Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] - BSA and Azide free
-
617 Alexa Fluor® 594
Alexa Fluor® 594 Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)]
-
519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)]
-
578 PE
PE Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)]
-
660 APC
APC Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)]
Reactivity data
Product details
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PDH-E2 plays a significant role in cellular energy production. It is an important part of the pyruvate dehydrogenase complex where its coordinated enzymatic activity drives the decarboxylation of pyruvate. This activity supplies acetyl-CoA for the citric acid cycle essential for ATP production. PDH-E2's function regulates the flow of carbon within cells linking glycolysis and the citric acid cycle making it essential for energy homeostasis. Its function integrates signals that regulate metabolic pathways helping maintain cellular energy balance.
Pathways
PDH-E2 integrates the glycolysis and citric acid cycle facilitating efficient energy production. It interacts with tightly linked enzymes in the pyruvate dehydrogenase complex important for catalyzing the conversion processes. Key related proteins include pyruvate dehydrogenase (E1) and dihydrolipoamide dehydrogenase (E3) which form the complex alongside E2. This complex is also critical in adjusting metabolic responses based on cellular energy needs efficiently managing substrate flux into the mitochondria.
Product protocols
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Target data
Publications (14)
Recent publications for all applications. Explore the full list and refine your search
Oncology research 33:617-630 PubMed40109870
2025
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Journal of translational medicine 23:162 PubMed39915841
2025
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Scientific reports 14:21926 PubMed39300184
2024
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The Journal of biological chemistry 299:105333 PubMed37827290
2023
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Nature communications 14:5114 PubMed37607904
2023
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eLife 12: PubMed36881526
2023
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Molecular therapy. Methods & clinical development 24:367-378 PubMed35252470
2022
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Frontiers in pharmacology 12:690371 PubMed34950023
2021
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Analytica chimica acta 1168:338609 PubMed34051998
2021
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Blood 137:2090-2102 PubMed33529321
2021
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com