Anti-Pyruvate dehydrogenase E2/E3bp antibody [13G2AE2BH5]

6 product images

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  This image is courtesy of a customer review submitted by Dimitra Kalamida

  Immunocytochemistry/ Immunofluorescence - Anti-Pyruvate dehydrogenase E2/E3bp antibody [13G2AE2BH5] (ab110333)

  ab110333 staining Pyruvate dehydrogenase E2/E3bp in Human HUVEC by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 pH 7.4 for 5 minutes and blocked with 5% BSA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/1000 in PBS) for 1 hour. A CF568-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.

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  Western blot - Anti-Pyruvate dehydrogenase E2/E3bp antibody [13G2AE2BH5] (ab110333)

  Lanes 1 - 4: Merged signal (red and green). Green - ab110333 observed at 72 kDa. Red - loading control, Anti-GAPDH antibody [EPR16891] - Loading Control ab181602, observed at 38 kDa.
  

  ab110333 was shown to specifically react with in wild-type HAP1 cells as signal was lost in DLAT knockout cells. Wild-type and DLAT knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab110333 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  All lanes: Western blot - Anti-Pyruvate dehydrogenase E2/E3bp antibody [13G2AE2BH5] (ab110333) at 1 µg/mL

  Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

  Lane 2: DLAT knockout HAP1 whole cell lysate at 20 µg

  Lane 3: HeLa whole cell lysate at 20 µg

  Lane 4: HL-60 whole cell lysate at 20 µg

  Predicted band size: 69 kDa

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  Western blot - Anti-Pyruvate dehydrogenase E2/E3bp antibody [13G2AE2BH5] (ab110333)

  All lanes: Western blot - Anti-Pyruvate dehydrogenase E2/E3bp antibody [13G2AE2BH5] (ab110333) at 1 µg/mL

  Lane 1: Isolated mitochondria from Human heart at 5 µg

  Lane 2: Isolated mitochondria from Bovine heart at 1 µg

  Lane 3: Isolated mitochondria from Rat heart at 10 µg

  Lane 4: Isolated mitochondria from Mouse heart at 10 µg

  Lane 5: HepG2 cell lysate at 20 µg

  Predicted band size: 69 kDa

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  Flow Cytometry - Anti-Pyruvate dehydrogenase E2/E3bp antibody [13G2AE2BH5] (ab110333)

  Flow cytometric analysis using ab110333 at 1µg/ml staining Pyruvate dehydrogenase E2/E3b in HL60 cells (blue). Isotype control antibody (red).

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  Immunocytochemistry/ Immunofluorescence - Anti-Pyruvate dehydrogenase E2/E3bp antibody [13G2AE2BH5] (ab110333)

  Immunocytochemistry analysis using ab110333 at 1μg/ml staining Pyruvate dehydrogenase E2/E3bp in cultured, normal Human embryonic lung fibroblasts and an AlexaFluor® 488 goat anti-mouse IgG2a secondary antibody (2 ug/ml).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pyruvate dehydrogenase E2/E3bp antibody [13G2AE2BH5] (ab110333)

  Immunohistological analysis using ab110333 at 1/1000 dilution staining Pyruvate dehydrogenase E2/E3bp in Human cerebellum tissue (Formalin-fixed, Paraffin-embedded).
  
  Note: Immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells.