Anti-QPRT antibody [EPR11941(B)]

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-QPRT antibody [EPR11941(B)] (AB171944)

Intracellular flow cytometric analysis of permeabilized Jurkat cells labeling QPRTwith ab171944 at 1/10 (red)or a rabbit IgG (negative) (green).

• IP

Lab

Immunoprecipitation - Anti-QPRT antibody [EPR11941(B)] (AB171944)

Immunoprecipitation of QPRT in HAP1 cells. Lysates were prepared and immunoprecipitation was performed using 2.0 µg of ab171944 pre-coupled to Protein A beads. Samples were washed and processed for western blot with ab171944 at 1/1000

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

Lane 1:

Immunoprecipitation - Anti-QPRT antibody [EPR11941(B)] (ab171944) at 1/1000 dilution

Lane 3:

Immunoprecipitation - Anti-QPRT antibody [EPR11941(B)] (ab171944) at 2 µg

All lanes:

HAP1 cells

Observed band size: 31 kDa

false

• WB

Lab

Western blot - Anti-QPRT antibody [EPR11941(B)] (AB171944)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : QPRT knockout HAP1 cell lysate (20 μg)
Lane 3 : HepG2 cell lysate (20 μg)
Lane 4 : Human fetal brain cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab171944 observed at 35 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab171944 was shown to specifically react with QPRT when QPRT knockout samples were used. Wild-type and QPRT knockout samples were subjected to SDS-PAGE. ab171944 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed ab216776 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-QPRT antibody [EPR11941(B)] (ab171944)

Predicted band size: 31 kDa

false

• WB

Supplier Data

Western blot - Anti-QPRT antibody [EPR11941(B)] (AB171944)

All lanes:

Western blot - Anti-QPRT antibody [EPR11941(B)] (ab171944) at 1/1000 dilution

Lane 1:

Human fetal kidney tissue lysate at 10 µg

Lane 2:

Human fetal liver tissue lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Lane 5:

Jurkat cell lysate at 10 µg

Lane 6:

Human fetal heart tissue lysate at 10 µg

Predicted band size: 31 kDa

true

• WB

Lab

Western blot - Anti-QPRT antibody [EPR11941(B)] (AB171944)

ab171944 was shown to react with QPRT in wild-type HAP1 cells in Western blot with loss of signal observed in a QPRT knockout cell line. Wild-type HAP1 and QPRT knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab171944 overnight at 4 °C at a 1/500 dilution. Blots were incubated with secondary antibodies at 0.2ug/mL before imaging.

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-QPRT antibody [EPR11941(B)] (ab171944) at 1/500 dilution

Lane 1:

Wild-type HAP1 lysate at 20 µg

Lane 2:

QPRT knock-out HAP1 lysate at 20 µg

Predicted band size: 31 kDa

false