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Rabbit Recombinant Monoclonal QPRT antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples.

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Images

Western blot - Anti-QPRT antibody [EPR11941(B)] - BSA and Azide free (AB249663), expandable thumbnail
  • Western blot - Anti-QPRT antibody [EPR11941(B)] - BSA and Azide free (AB249663), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-QPRT antibody [EPR11941(B)] - BSA and Azide free (AB249663), expandable thumbnail
  • Western blot - Anti-QPRT antibody [EPR11941(B)] - BSA and Azide free (AB249663), expandable thumbnail
  • Immunoprecipitation - Anti-QPRT antibody [EPR11941(B)] - BSA and Azide free (AB249663), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPIHCWBICC/IFFlow Cyt (Intra)
Human
Tested
Not recommended
Tested
Not recommended
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Involved in the catabolism of quinolinic acid (QA).

Alternative names

Nicotinate-nucleotide pyrophosphorylase [carboxylating], Quinolinate phosphoribosyltransferase [decarboxylating], QAPRTase, QPRTase, QPRT

Recommended products

Rabbit Recombinant Monoclonal QPRT antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR11941(B)
Purification technique
Affinity purification
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab249663 is the carrier-free version of Anti-QPRT antibody [EPR11941(B)] ab171944.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

QPRT also known as Nicotinate-nucleotide pyrophosphorylase [carboxylating] or Quinolinate phosphoribosyltransferase functions as an important enzyme in the kynurenine pathway a metabolic pathway leading to the production of nicotinamide adenine dinucleotide (NAD+). The enzyme catalyzes the conversion of quinolinate to nicotinic acid mononucleotide. QPRT has a molecular mass of approximately 34 kDa. It is expressed in various tissues most notably in the liver kidney and brain.

Biological function summary

QPRT influences the synthesis of NAD+ which is essential for cellular redox reactions and energy metabolism. The enzyme operates as a monomer and does not typically form part of a larger complex. However its activity is important in maintaining NAD+ levels which can impact a wide range of cellular processes such as DNA repair signal transduction and metabolism.

Pathways

QPRT holds a significant role in the kynurenine pathway and NAD+ biosynthesis. This pathway includes several enzymes such as kynurenine 3-monooxygenase and kynureninase which collaboratively contribute to the degradation of tryptophan ultimately affecting the levels of important coenzymes and neurotransmitters. The NAD+ biosynthesis pathway involving QPRT also interacts with pathways for ATP generation connecting with other metabolic processes.

Associated diseases and disorders

QPRT's malfunction links to neurological disorders such as Alzheimer's disease due to its role in NAD+ metabolism and potential effects on neuronal health and energy supply. Additionally alterations in QPRT activity have associations with liver diseases considering its expression and function in metabolic pathways within hepatic tissues. Interaction with proteins like kynurenine aminotransferase highlights its involvement in maintaining metabolic balance within these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-QPRT antibody [EPR11941(B)] - BSA and Azide free (ab249663), expandable thumbnail

    Western blot - Anti-QPRT antibody [EPR11941(B)] - BSA and Azide free (ab249663)

    This data was developed using Anti-QPRT antibody [EPR11941(B)] ab171944, the same antibody clone in a different buffer formulation.

    Lane 1 Wild-type HAP1 cell lysate (20 μg)

    Lane 2 QPRT knockout HAP1 cell lysate (20 μg)

    Lane 3 HepG2 cell lysate (20 μg)

    Lane 4 Human fetal brain cell lysate (20 μg)

    Lanes 1 - 4 Merged signal (red and green). Green - Anti-QPRT antibody [EPR11941(B)] ab171944 observed at 35 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    Anti-QPRT antibody [EPR11941(B)] ab171944 was shown to specifically react with QPRT when QPRT knockout samples were used. Wild-type and QPRT knockout samples were subjected to SDS-PAGE. Anti-QPRT antibody [EPR11941(B)] ab171944 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-QPRT antibody [EPR11941(B)] (Anti-QPRT antibody [EPR11941(B)] ab171944)

    Predicted band size: 31 kDa

  • Western blot - Anti-QPRT antibody [EPR11941(B)] - BSA and Azide free (ab249663), expandable thumbnail

    Western blot - Anti-QPRT antibody [EPR11941(B)] - BSA and Azide free (ab249663)

    This data was developed using Anti-QPRT antibody [EPR11941(B)] ab171944, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-QPRT antibody [EPR11941(B)] (Anti-QPRT antibody [EPR11941(B)] ab171944) at 1/1000 dilution

    Lane 1: Human fetal kidney tissue lysate at 10 µg

    Lane 2: Human fetal liver tissue lysate at 10 µg

    Lane 3: HepG2 cell lysate at 10 µg

    Lane 4: HeLa cell lysate at 10 µg

    Lane 5: Jurkat cell lysate at 10 µg

    Lane 6: Human fetal heart tissue lysate at 10 µg

    Developed using the ECL technique.

    Predicted band size: 31 kDa

  • Flow Cytometry (Intracellular) - Anti-QPRT antibody [EPR11941(B)] - BSA and Azide free (ab249663), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-QPRT antibody [EPR11941(B)] - BSA and Azide free (ab249663)

    This data was developed using Anti-QPRT antibody [EPR11941(B)] ab171944, the same antibody clone in a different buffer formulation.

    Intracellular flow cytometric analysis of permeabilized Jurkat cells labeling QPRT with Anti-QPRT antibody [EPR11941(B)] ab171944 at 1/10 (red) or a rabbit IgG (negative) (green).

  • Western blot - Anti-QPRT antibody [EPR11941(B)] - BSA and Azide free (ab249663), expandable thumbnail

    Western blot - Anti-QPRT antibody [EPR11941(B)] - BSA and Azide free (ab249663)

    This data was developed using the same antibody clone in a different buffer formulation (Anti-QPRT antibody [EPR11941(B)] ab171944)


    Anti-QPRT antibody [EPR11941(B)] ab171944 was shown to react with QPRT in wild-type HAP1 cells in Western blot with loss of signal observed in a QPRT knockout cell line. Wild-type HAP1 and QPRT knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with Anti-QPRT antibody [EPR11941(B)] ab171944 overnight at 4 °C at a 1/500 dilution. Blots were incubated with secondary antibodies at 0.2ug/mL before imaging.

    This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

    All lanes: Western blot - Anti-QPRT antibody [EPR11941(B)] (Anti-QPRT antibody [EPR11941(B)] ab171944) at 1/500 dilution

    Lane 1: Wild-type HAP1 lysate at 20 µg

    Lane 2: QPRT knock-out HAP1 lysate at 20 µg

    Predicted band size: 31 kDa

  • Immunoprecipitation - Anti-QPRT antibody [EPR11941(B)] - BSA and Azide free (ab249663), expandable thumbnail

    Immunoprecipitation - Anti-QPRT antibody [EPR11941(B)] - BSA and Azide free (ab249663)

    This data was developed using the same antibody clone in a different buffer formulation (Anti-QPRT antibody [EPR11941(B)] ab171944)


    Immunoprecipitation of QPRT in HAP1 cells. Lysates were prepared and immunoprecipitation was performed using 2.0 µg of Anti-QPRT antibody [EPR11941(B)] ab171944 pre-coupled to Protein A beads. Samples were washed and processed for western blot with Anti-QPRT antibody [EPR11941(B)] ab171944 at 1/1000

    This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

    Lane 1: Immunoprecipitation - Anti-QPRT antibody [EPR11941(B)] (Anti-QPRT antibody [EPR11941(B)] ab171944) at 1/1000 dilution

    Lane 3: Immunoprecipitation - Anti-QPRT antibody [EPR11941(B)] (Anti-QPRT antibody [EPR11941(B)] ab171944) at 2 µg

    All lanes: HAP1 cells

    Observed band size: 31 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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