Anti-Quiescin Q6 antibody [EPR21866]

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Quiescin Q6 antibody [EPR21866] (AB235444)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized MDA-MB-231 (Human breast adenocarcinoma cell line) cells labeling Quiescin Q6 with ab235444 at 1/60 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quiescin Q6 antibody [EPR21866] (AB235444)

Immunohistochemical analysis of paraffin-embedded human breast ductal cancer tissue labeling Quiescin Q6 with ab235444 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining in human breast ductal cancer (PMID : 23536962) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quiescin Q6 antibody [EPR21866] (AB235444)

Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue labeling Quiescin Q6 with ab235444 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining in human pancreatic cancer (PMID : 19795908, 21989104) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• IP

Unknown

Immunoprecipitation - Anti-Quiescin Q6 antibody [EPR21866] (AB235444)

Quiescin Q6 was immunoprecipitated from 0.35 mg of MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell lysate with ab235444 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab235444 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1 : MDA-MB-231 whole cell lysate 10 μg (Input).
Lane 2 : ab235444 IP in MDA-MD-231 whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab235444 in MDA-MD-231 whole cell lysate.

Blocking/Dilution buffer : 5% NFDM/TBST.
Exposure time : 30 seconds.

Quiescin Q6 has two isoforms due to expression from a long (Quiescin Q6-L) and a short (Quiescin Q6-S) transcript.

All lanes:

Immunoprecipitation - Anti-Quiescin Q6 antibody [EPR21866] (ab235444)

Predicted band size: 83 kDa

Observed band size: 65 kDa,83 kDa

false

• IP

Supplier Data

Immunoprecipitation - Anti-Quiescin Q6 antibody [EPR21866] (AB235444)

Quiescin Q6 was immunoprecipitated from 0.35 mg ab323533 at 1/30 IP in PANC-1(human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate with ab323533 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab323533 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

The IP experiment was performed by ab323533 using PANC-1 lysate. The blot was probed by another Anti-Quiescin Q6 antibody [EPR21866] (ab235444)(1 : 1000).

All lanes:

Immunoprecipitation - Anti-Quiescin Q6 antibody [EPR25653-56] (<a href='/en-us/products/primary-antibodies/quiescin-q6-antibody-epr25653-56-ab323533'>ab323533</a>) at 1/1000 dilution

Lane 1:

PANC-1(human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate at 10 µg

Lane 2:

<a href='/en-us/products/primary-antibodies/quiescin-q6-antibody-epr25653-56-ab323533'>ab323533</a> at 1/30 IP in PANC-1(human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate at 10 µg

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/en-us/products/primary-antibodies/quiescin-q6-antibody-epr25653-56-ab323533'>ab323533</a> in PANC-1 whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 180s

• WB

Unknown

Western blot - Anti-Quiescin Q6 antibody [EPR21866] (AB235444)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lanes 1-3 & 5 : 3 minutes, Lane 4 : 37 seconds, Lane 6 : 103 seconds.

Quiescin Q6 has two isoforms due to expression from a long (Quiescin Q6-L) and a short (Quiescin Q6-S) transcript.

All lanes:

Western blot - Anti-Quiescin Q6 antibody [EPR21866] (ab235444) at 1/1000 dilution

Lane 1:

BxPC-3 (human pancreas adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 2:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 3:

MDA-MB-231 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 4:

PANC-1 (human pancreatic epithelial cancinoma cell line) whole cell lysate at 10 µg

Lane 5:

T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate at 10 µg

Lane 6:

Human breast cancer lysate at 10 µg

Secondary

Lanes 1 - 5:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lane 6:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 83 kDa

Observed band size: 67 kDa,83 kDa

false