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AB315915

Anti-Quiescin Q6 antibody [EPR25653-17] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal Quiescin Q6 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

QSCN6, UNQ2520/PRO6013, QSOX1, Sulfhydryl oxidase 1, hQSOX, Quiescin Q6

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Sandwich ELISA - Anti-Quiescin Q6 antibody [EPR25653-17] - BSA and Azide free (Capture) (AB315915)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Quiescin Q6 antibody [EPR25653-17] - BSA and Azide free (Capture) (AB315915)

Sandwich ELISA of ab315914 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25653-17

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab315915 is a BSA and Azide free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Quiescin Q6. The recommended pair for sandwich ELISA is:

Capture: ab315915, Human Quiescin Q6 Capture Antibody (unconjugated)

Detector: ab315916, Human Quiescin Q6 Detector Antibody (unconjugated)

The reference range value is 31.25-2000 pg/mL.

The recommended antibody orientation is based on internal optimization for ELISA based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Quiescin Q6 also known as QSOX1 is an enzyme belonging to the Quiescin-sulfhydryl oxidase family. It functions by catalyzing the oxidation of sulfhydryl groups to form disulfide bonds in proteins an important step for proper protein folding. QSOX1 has a molecular mass between 60-100 kDa depending on its isoform. This protein is expressed in various tissues with high levels present in the pancreas placenta and some cancer cells suggesting a role in tissue specific processes and during certain pathological conditions.
Biological function summary

Quiescin Q6 influences cell growth and differentiation. The protein is involved in extracellular matrix organization where it plays a role in maintaining the stability and function of connective tissues. It doesn't typically assemble into large complexes but interacts with substrates directly facilitating the formation of stable proteins. QSOX1 ensures that proteins achieve their functional conformation by reshaping their disulfide bonds efficiently aiding in the cellular machinery of protein conformation.

Pathways

The functions of Quiescin Q6 intersect with the protein folding and oxidative stress response pathways. It relates closely to the endoplasmic reticulum protein folding pathway due to its role in disulfide bonding as well as to the antioxidant defense pathway where it mediates protection against oxidative stress. Its activity connects with proteins such as protein disulfide isomerase (PDI) which also participate in creating disulfide bonds but at different stages or cellular locations showcasing distinct but cooperative functions within these pathways.

Quiescin Q6 has been linked to cancer progression and fibrosis. Its overexpression has been observed in various tumors indicating a potential role in tumor growth and metastasis. In fibrotic diseases elevated levels of QSOX1 contribute to excessive extracellular matrix deposition. It connects with the transforming growth factor-beta (TGF-beta) pathway a critical regulator in fibrosis and cancer where QSOX1's regulation of matrix composition may influence TGF-beta signaling. Understanding these connections offers potential for developing targeted therapeutic strategies in treating related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the oxidation of sulfhydryl groups in peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide (PubMed : 17331072, PubMed : 18393449, PubMed : 23704371, PubMed : 23867277, PubMed : 30367560). Plays a role in disulfide bond formation in a variety of extracellular proteins (PubMed : 17331072, PubMed : 22801504, PubMed : 23867277, PubMed : 30367560). In fibroblasts, required for normal incorporation of laminin into the extracellular matrix, and thereby for normal cell-cell adhesion and cell migration (PubMed : 23704371, PubMed : 23867277, PubMed : 30367560).
See full target information QSOX1
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Product promise

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