Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24]

17 product images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] (ab271094)

  Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labelling Rab 6A + Rab 6D + Ral-A with ab271094 at 1/2000 (0.296 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Granular cytoplasmic staining on mouse testis. The section was incubated with ab271094 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• 

  Immunoprecipitation - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] (ab271094)

  Rab 6A was immunoprecipitated from 0.35 mg Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate with ab271094 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab271094 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
  

  Lane 1: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 10 ug

  Lane 2: ab271094 IP in Neuro-2a whole cell lysate

  Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab271094 in Neuro-2a whole cell lysate

  Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  Exposure time: 10 seconds.

  All lanes: Immunoprecipitation - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] (ab271094)

  Predicted band size: 24 kDa

  Observed band size: 24 kDa

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] (ab271094)

  Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized Neuro-2a cells labelling Rab 6A + Rab 6D + Ral-A with ab271094 at 1/50 (11.84 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing golgi staining in Neuro-2a cell line. Anti-GM130 antibody - BSA and Azide free ab169276 Anti-GM130 mouse polyclonal antibody - cis-Golgi Marker was used to counterstain GM130 at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
  

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.

• 

  Western blot - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] (ab271094)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.
  

  Exposure time: 26 seconds.

  All lanes: Western blot - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] (ab271094) at 1/1000 dilution

  Lane 1: PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg

  Lane 2: HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

  Lane 3: HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg

  Lane 4: HEK-293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

  Lane 5: A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

  Lane 6: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

  Lane 7: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

  Lane 8: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

  Lane 9: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Predicted band size: 24 kDa

  Observed band size: 24 kDa

• 

  Flow Cytometry (Intracellular) - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] (ab271094)

  Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized A549 (Human lung carcinoma epithelial cell) cells labelling Rab 6A + Rab 6D + Ral-A with ab271094 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] (ab271094)

  Immunohistochemical analysis of paraffin-embedded Human spleen tissue labelling Rab 6A + Rab 6D + Ral-A with ab271094 at 1/2000 (0.296 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Granular cytoplasmic staining on human spleen. The section was incubated with ab271094 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
  

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] (ab271094)

  Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized A549 cells labelling Rab 6A + Rab 6D + Ral-A with ab271094 at 1/50 (11.84 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing golgi staining in A549 cell line. Anti-GM130 antibody - BSA and Azide free ab169276 Anti-GM130 mouse polyclonal antibody - cis-Golgi Marker was used to counterstain the GM-130 at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
  

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.

• 

  Western blot - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] (ab271094)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.
  

  Exposure time: Lane 1-2: 15 secondsLane 3: 48 seconds.

  All lanes: Western blot - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] (ab271094) at 1/1000 dilution

  Lane 1: Human brain tissue lysate at 20 µg

  Lane 2: Human heart tissue lysate at 20 µg

  Lane 3: Human kidney tissue lysate at 20 µg

  Secondary

  All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

  Predicted band size: 24 kDa

  Observed band size: 24 kDa

• 

  Flow Cytometry (Intracellular) - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] (ab271094)

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Neuro-2a (Mouse neuroblastoma neuroblast) cells labelling Rab 6A + Rab 6D + Ral-A with ab271094 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

• 

  Immunoprecipitation - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] (ab271094)

  Rab 6A was immunoprecipitated from 0.35 mg A549 (human lung carcinoma epithelial cell) whole cell lysate with ab271094 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab271094 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
  

  Lane 1: A549 (human lung carcinoma epithelial cell) whole cell lysate 10 ug

  Lane 2: ab271094 IP in A549 whole cell lysate

  Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab271094 in A549 whole cell lysate

  Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  Exposure time: 10 seconds.

  All lanes: Immunoprecipitation - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] (ab271094)

  Predicted band size: 24 kDa

  Observed band size: 24 kDa

• 

  Western blot - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] (ab271094)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.
  

  Exposure time: 48 seconds.

  All lanes: Western blot - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] (ab271094) at 1/1000 dilution

  Lane 1: Mouse brain tissue lysate at 20 µg

  Lane 2: Mouse heart tissue lysate at 20 µg

  Lane 3: Mouse kidney tissue lysate at 20 µg

  Lane 4: Mouse spleen tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Predicted band size: 24 kDa

  Observed band size: 24 kDa

• 

  Western blot - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] (ab271094)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.
  

  Exposure time: Lane 1, 3, 4: 48 secondsLane 2: 3 minutes.

  All lanes: Western blot - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] (ab271094) at 1/1000 dilution

  Lane 1: Rat brain tissue lysate at 20 µg

  Lane 2: Rat heart tissue lysate at 20 µg

  Lane 3: Rat kidney tissue lysate at 20 µg

  Lane 4: Rat spleen tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Predicted band size: 24 kDa

  Observed band size: 24 kDa

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] (ab271094)

  Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labelling Rab 6A + Rab 6D + Ral-A with ab271094 at 1/2000 (0.296 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Granular cytoplasmic staining on rat spleen. The section was incubated with ab271094 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument .Counterstained with Hematoxylin.
  

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• 

  Western blot - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] (ab271094)

  Blocking and diluting buffer and concentration: 5% NFDM /TBST

  All lanes: Western blot - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] (ab271094) at 1/1000 dilution

  Lane 1: 293T cells transfected with an empty vector containing a His tag whole cell lysate at 20 µg

  Lane 2: 293T cells transfected with a human Rab6A expression vector containing a His-tag whole cell lysate at 20 µg

  Lane 3: 293T cells transfected with a human Ral-A expression vector containing a His-tag whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Observed band size: 26 kDa

  Exposure time: 1s

• 

  Multiplex immunohistochemistry - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] (ab271094)

  Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat colon tissue staining FUT8 with Anti-FUT8 antibody [EPR29984-720] ab322558 at a 1:500 (1.026 ug/ml) dilution, ab271094 anti-Rab 6A + Rab 6D + Ral-A used at 1:2000 (0.296 ug/ml) dilution.

  Panel A: merged staining of anti-FUT8 (green; Opal™520) and anti-Rab 6A + Rab 6D + Ral-A (magenta; Opal™570) on rat colon.
  
  Panel B: anti-GUT8 showed granular staining in rat colon.
  
  Panel C: ant-Rab 6A + Rab 6D + Ral-A showed granular staining in rat colon.
  
  Panel D: Nuclear DNA was labeled with DAPI (shown in blue).

  The section was incubated in three rounds of staining: in the order of Anti-FUT8 antibody [EPR29984-720] ab322558 and ab271094 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  Nuclear counter stain with DAPI.

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• 

  Multiplex immunohistochemistry - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] (ab271094)

  Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human stomach tissue staining FUT8 with Anti-FUT8 antibody [EPR29984-720] ab322558 at a 1:500 (1.026 ug/ml) dilution, ab271094 anti-Rab 6A + Rab 6D + Ral-A used at 1:2000 (0.296 ug/ml) dilution.

  Panel A: merged staining of anti-FUT8 (green; Opal™520) and anti-Rab 6A + Rab 6D + Ral-A (magenta; Opal™570) on human stomach.
  
  Panel B: anti-GUT8 showed granular staining in human stomach.
  
  Panel C: ant-Rab 6A + Rab 6D + Ral-A showed granular staining in human stomach.
  
  Panel D: Nuclear DNA was labeled with DAPI (shown in blue).

  The section was incubated in three rounds of staining: in the order of Anti-FUT8 antibody [EPR29984-720] ab322558 and ab271094 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  Nuclear counter stain with DAPI.

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• 

  Multiplex immunohistochemistry - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] (ab271094)

  Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse colon tissue staining FUT8 with Anti-FUT8 antibody [EPR29984-720] ab322558 at a 1:500 (1.026 ug/ml) dilution, ab271094 anti-Rab 6A + Rab 6D + Ral-A used at 1:2000 (0.296 ug/ml) dilution.

  Panel A: merged staining of anti-FUT8 (green; Opal™520) and anti-Rab 6A + Rab 6D + Ral-A (magenta; Opal™570) on mouse colon.
  
  Panel B: anti-GUT8 showed granular staining in mouse colon.
  
  Panel C: ant-Rab 6A + Rab 6D + Ral-A showed granular staining in mouse colon.
  
  Panel D: Nuclear DNA was labeled with DAPI (shown in blue).

  The section was incubated in three rounds of staining: in the order of Anti-FUT8 antibody [EPR29984-720] ab322558 and ab271094 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  Nuclear counter stain with DAPI.

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.