Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (ab280955)

Rabbit Recombinant Monoclonal RAB6A antibody. Carrier free. Suitable for WB, ICC/IF, IP, Flow Cyt (Intra), IHC-P and reacts with Transfected cell lysate - Human, Mouse, Rat, Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (AB280955), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	ICC/IF	IP	IHC-Fr	Flow Cyt (Intra)	IHC-P
Human	Tested	Tested	Tested	Not recommended	Tested	Tested
Mouse	Tested	Tested	Tested	Not recommended	Tested	Tested
Rat	Tested	Expected	Expected	Not recommended	Expected	Tested
Transfected cell lysate - Human	Tested	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Transfected cell lysate - Human, Mouse, Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human, Transfected cell lysate - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Human	Dilution info -	Notes -

Target data

See full target information RAB6A

Function

Regulator of COPI-independent retrograde transport from the Golgi apparatus towards the endoplasmic reticulum (ER) (PubMed:25962623). Has a low GTPase activity (PubMed:25962623). Recruits VPS13B to the Golgi membrane (PubMed:25492866). Plays a role in neuron projection development (Probable).

Additional Targets

RALA, RAB6D

Alternative names

RAB6, RAB6A, Ras-related protein Rab-6A, Rab-6

Recommended products

Rabbit Recombinant Monoclonal RAB6A antibody. Carrier free. Suitable for WB, ICC/IF, IP, Flow Cyt (Intra), IHC-P and reacts with Transfected cell lysate - Human, Mouse, Rat, Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR24472-24
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

ab280955 is the carrier-free version of Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] ab271094.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Rab 6A also known as Ras-related protein Rab-6A is a member of the Rab family of small GTPases and has a molecular weight of approximately 24 kDa. This protein plays an essential role in intracellular transport mechanisms particularly overseeing the trafficking of proteins and lipids between the Golgi apparatus and the endoplasmic reticulum. Rab 6A is expressed in various tissues but is found predominantly in neuronal tissues where it has vital functions.

Biological function summary

This protein is involved in regulating membrane trafficking and maintains Golgi apparatus structure. Rab 6A engages with several other proteins to form complexes necessary for the vesicular transport processes. It aids in coordinating the interactions between transport vesicles and their target membranes directly impacting cellular homeostasis and protein export.

Pathways

Rab 6A operates significantly in the secretory pathway as well as the Golgi retrograde transport pathway. Within the secretory pathway it directly affects protein sorting and recycling. Additionally Rab 6A interacts with proteins such as Bicaudal-D and dynactin which link it to the recruitment and movement of vesicular cargo along the microtubules integrating it into the broader network of intracellular pathway regulation.

Associated diseases and disorders

Rab 6A has connections with neurological conditions. Its dysfunction or misregulation can impact neurodegenerative diseases such as Alzheimer's disease by disrupting protein processing and transport. Rab 6A interacts with amyloid precursor protein-processing pathways emphasizing its role in disease pathways. Also Rab 6A is linked to disorders affecting the Golgi apparatus a critical site for protein post-translational modification and trafficking within cells.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

14 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (ab280955)
This data was developed using Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] ab271094, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labelling Rab 6A + Rab 6D + Ral-A with Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] ab271094 at 1/2000 (0.296 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Granular cytoplasmic staining on mouse testis. The section was incubated with Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] ab271094 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunoprecipitation - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (ab280955)
This data was developed using Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] ab271094, the same antibody clone in a different buffer formulation.
Rab 6A was immunoprecipitated from 0.35 mg Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate with Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] ab271094 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] ab271094 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 10 ug
Lane 2: Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] ab271094 IP in Neuro-2a whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] ab271094 in Neuro-2a whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
All lanes: Immunoprecipitation - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] (Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] ab271094)
Predicted band size: 24 kDa
Observed band size: 24 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (ab280955)
This data was developed using Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] ab271094, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized Neuro-2a cells labelling Rab 6A + Rab 6D + Ral-A with Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] ab271094 at 1/50 (11.84 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing golgi staining in Neuro-2a cell line. Anti-GM130 antibody - BSA and Azide free ab169276 Anti-GM130 mouse polyclonal antibody - cis-Golgi Marker was used to counterstain GM130 at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.
Western blot - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (ab280955)
This data was developed using Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] ab271094, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 26 seconds.
All lanes: Western blot - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] (Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] ab271094) at 1/1000 dilution
Lane 1: PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3: HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: HEK-293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 5: A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 6: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg
Lane 7: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 8: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg
Lane 9: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDa
Flow Cytometry (Intracellular) - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (ab280955)
This data was developed using Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] ab271094, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized A549 (Human lung carcinoma epithelial cell) cells labelling Rab 6A + Rab 6D + Ral-A with Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] ab271094 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (ab280955)
This data was developed using Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] ab271094, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labelling Rab 6A + Rab 6D + Ral-A with Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] ab271094 at 1/2000 (0.296 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Granular cytoplasmic staining on human spleen. The section was incubated with Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] ab271094 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunocytochemistry/ Immunofluorescence - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (ab280955)
This data was developed using Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] ab271094, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized A549 cells labelling Rab 6A + Rab 6D + Ral-A with Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] ab271094 at 1/50 (11.84 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing golgi staining in A549 cell line. Anti-GM130 antibody - BSA and Azide free ab169276 Anti-GM130 mouse polyclonal antibody - cis-Golgi Marker was used to counterstain the GM-130 at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.
Western blot - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (ab280955)
This data was developed using Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] ab271094, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: Lane 1-2: 15 secondsLane 3: 48 seconds.
All lanes: Western blot - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] (Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] ab271094) at 1/1000 dilution
Lane 1: Human brain tissue lysate at 20 µg
Lane 2: Human heart tissue lysate at 20 µg
Lane 3: Human kidney tissue lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDa
Flow Cytometry (Intracellular) - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (ab280955)
This data was developed using Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] ab271094, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Neuro-2a (Mouse neuroblastoma neuroblast) cells labelling Rab 6A + Rab 6D + Ral-A with Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] ab271094 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (ab280955)
This data was developed using Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] ab271094, the same antibody clone in a different buffer formulation.
Rab 6A was immunoprecipitated from 0.35 mg A549 (human lung carcinoma epithelial cell) whole cell lysate with Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] ab271094 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] ab271094 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: A549 (human lung carcinoma epithelial cell) whole cell lysate 10 ug
Lane 2: Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] ab271094 IP in A549 whole cell lysate
Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] ab271094 in A549 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
All lanes: Immunoprecipitation - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] (Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] ab271094)
Predicted band size: 24 kDa
Observed band size: 24 kDa
Western blot - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (ab280955)
This data was developed using Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] ab271094, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 48 seconds.
All lanes: Western blot - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] (Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] ab271094) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse heart tissue lysate at 20 µg
Lane 3: Mouse kidney tissue lysate at 20 µg
Lane 4: Mouse spleen tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDa
Western blot - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (ab280955)
This data was developed using Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] ab271094, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: Lane 1, 3, 4: 48 secondsLane 2: 3 minutes.
All lanes: Western blot - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] (Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] ab271094) at 1/1000 dilution
Lane 1: Rat brain tissue lysate at 20 µg
Lane 2: Rat heart tissue lysate at 20 µg
Lane 3: Rat kidney tissue lysate at 20 µg
Lane 4: Rat spleen tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (ab280955)
This data was developed using Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] ab271094, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labelling Rab 6A + Rab 6D + Ral-A with Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] ab271094 at 1/2000 (0.296 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Granular cytoplasmic staining on rat spleen. The section was incubated with Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] ab271094 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument .Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Western blot - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (ab280955)
Blocking and diluting buffer and concentration: 5% NFDM /TBST
All lanes: Western blot - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] (Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] ab271094) at 1/1000 dilution
Lane 1: 293T cells transfected with an empty vector containing a His tag whole cell lysate at 20 µg
Lane 2: 293T cells transfected with a human Rab6A expression vector containing a His-tag whole cell lysate at 20 µg
Lane 3: 293T cells transfected with a human Ral-A expression vector containing a His-tag whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 26 kDa
Exposure time: 1s