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AB280955

Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free

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Rabbit Recombinant Monoclonal RAB6A antibody. Carrier free. Suitable for WB, ICC/IF, IP, Flow Cyt (Intra), IHC-P and reacts with Transfected cell lysate - Human, Mouse, Rat, Human samples.

View Alternative Names

RAB6, RAB6A, Ras-related protein Rab-6A, Rab-6

14 Images
Flow Cytometry (Intracellular) - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (AB280955)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (AB280955)

This data was developed using ab271094, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized A549 (Human lung carcinoma epithelial cell) cells labelling Rab 6A + Rab 6D + Ral-A with ab271094 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (AB280955)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (AB280955)

This data was developed using ab271094, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized A549 cells labelling Rab 6A + Rab 6D + Ral-A with ab271094 at 1/50 (11.84 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing golgi staining in A549 cell line. ab169276 Anti-GM130 mouse polyclonal antibody - cis-Golgi Marker was used to counterstain the GM-130 at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (AB280955)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (AB280955)

This data was developed using ab271094, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labelling Rab 6A + Rab 6D + Ral-A with ab271094 at 1/2000 (0.296 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Granular cytoplasmic staining on human spleen. The section was incubated with ab271094 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunoprecipitation - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (AB280955)
  • IP

Supplier Data

Immunoprecipitation - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (AB280955)

This data was developed using ab271094, the same antibody clone in a different buffer formulation.

Rab 6A was immunoprecipitated from 0.35 mg A549 (human lung carcinoma epithelial cell) whole cell lysate with ab271094 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab271094 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : A549 (human lung carcinoma epithelial cell) whole cell lysate 10 ug

Lane 2 : ab271094 IP in A549 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab271094 in A549 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] (<a href='/en-us/products/primary-antibodies/rab-6a-rab-6d-ral-a-antibody-epr24472-24-ab271094'>ab271094</a>)

Predicted band size: 24 kDa

Observed band size: 24 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (AB280955)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (AB280955)

This data was developed using ab271094, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labelling Rab 6A + Rab 6D + Ral-A with ab271094 at 1/2000 (0.296 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Granular cytoplasmic staining on rat spleen. The section was incubated with ab271094 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument .Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunocytochemistry/ Immunofluorescence - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (AB280955)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (AB280955)

This data was developed using ab271094, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized Neuro-2a cells labelling Rab 6A + Rab 6D + Ral-A with ab271094 at 1/50 (11.84 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing golgi staining in Neuro-2a cell line. ab169276 Anti-GM130 mouse polyclonal antibody - cis-Golgi Marker was used to counterstain GM130 at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (AB280955)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (AB280955)

This data was developed using ab271094, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labelling Rab 6A + Rab 6D + Ral-A with ab271094 at 1/2000 (0.296 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Granular cytoplasmic staining on mouse testis. The section was incubated with ab271094 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Flow Cytometry (Intracellular) - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (AB280955)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (AB280955)

This data was developed using ab271094, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Neuro-2a (Mouse neuroblastoma neuroblast) cells labelling Rab 6A + Rab 6D + Ral-A with ab271094 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Immunoprecipitation - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (AB280955)
  • IP

Supplier Data

Immunoprecipitation - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (AB280955)

This data was developed using ab271094, the same antibody clone in a different buffer formulation.

Rab 6A was immunoprecipitated from 0.35 mg Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate with ab271094 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab271094 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 10 ug

Lane 2 : ab271094 IP in Neuro-2a whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab271094 in Neuro-2a whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] (<a href='/en-us/products/primary-antibodies/rab-6a-rab-6d-ral-a-antibody-epr24472-24-ab271094'>ab271094</a>)

Predicted band size: 24 kDa

Observed band size: 24 kDa

false

Western blot - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (AB280955)
  • WB

Lab

Western blot - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (AB280955)

This data was developed using ab271094, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 26 seconds.

All lanes:

Western blot - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] (<a href='/en-us/products/primary-antibodies/rab-6a-rab-6d-ral-a-antibody-epr24472-24-ab271094'>ab271094</a>) at 1/1000 dilution

Lane 1:

PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

HEK-293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 5:

A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 6:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Lane 7:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 8:

PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Lane 9:

C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 24 kDa

Observed band size: 24 kDa

false

Western blot - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (AB280955)
  • WB

Lab

Western blot - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (AB280955)

This data was developed using ab271094, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : Lane 1-2 : 15 secondsLane 3 : 48 seconds.

All lanes:

Western blot - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] (<a href='/en-us/products/primary-antibodies/rab-6a-rab-6d-ral-a-antibody-epr24472-24-ab271094'>ab271094</a>) at 1/1000 dilution

Lane 1:

Human brain tissue lysate at 20 µg

Lane 2:

Human heart tissue lysate at 20 µg

Lane 3:

Human kidney tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 24 kDa

Observed band size: 24 kDa

false

Western blot - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (AB280955)
  • WB

Lab

Western blot - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (AB280955)

This data was developed using ab271094, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : Lane 1, 3, 4 : 48 secondsLane 2 : 3 minutes.

All lanes:

Western blot - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] (<a href='/en-us/products/primary-antibodies/rab-6a-rab-6d-ral-a-antibody-epr24472-24-ab271094'>ab271094</a>) at 1/1000 dilution

Lane 1:

Rat brain tissue lysate at 20 µg

Lane 2:

Rat heart tissue lysate at 20 µg

Lane 3:

Rat kidney tissue lysate at 20 µg

Lane 4:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 24 kDa

Observed band size: 24 kDa

false

Western blot - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (AB280955)
  • WB

Lab

Western blot - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (AB280955)

This data was developed using ab271094, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 48 seconds.

All lanes:

Western blot - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] (<a href='/en-us/products/primary-antibodies/rab-6a-rab-6d-ral-a-antibody-epr24472-24-ab271094'>ab271094</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Lane 3:

Mouse kidney tissue lysate at 20 µg

Lane 4:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 24 kDa

Observed band size: 24 kDa

false

Western blot - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (AB280955)
  • WB

Lab

Western blot - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] - BSA and Azide free (AB280955)

Blocking and diluting buffer and concentration : 5% NFDM /TBST

All lanes:

Western blot - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] (<a href='/en-us/products/primary-antibodies/rab-6a-rab-6d-ral-a-antibody-epr24472-24-ab271094'>ab271094</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a His tag whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human Rab6A expression vector containing a His-tag whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human Ral-A expression vector containing a His-tag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 26 kDa

false

Exposure time: 1s

  • Unconjugated

    Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24472-24

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), IHC-P, WB, IP, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab280955 is the carrier-free version of ab271094.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rab 6A also known as Ras-related protein Rab-6A is a member of the Rab family of small GTPases and has a molecular weight of approximately 24 kDa. This protein plays an essential role in intracellular transport mechanisms particularly overseeing the trafficking of proteins and lipids between the Golgi apparatus and the endoplasmic reticulum. Rab 6A is expressed in various tissues but is found predominantly in neuronal tissues where it has vital functions.
Biological function summary

This protein is involved in regulating membrane trafficking and maintains Golgi apparatus structure. Rab 6A engages with several other proteins to form complexes necessary for the vesicular transport processes. It aids in coordinating the interactions between transport vesicles and their target membranes directly impacting cellular homeostasis and protein export.

Pathways

Rab 6A operates significantly in the secretory pathway as well as the Golgi retrograde transport pathway. Within the secretory pathway it directly affects protein sorting and recycling. Additionally Rab 6A interacts with proteins such as Bicaudal-D and dynactin which link it to the recruitment and movement of vesicular cargo along the microtubules integrating it into the broader network of intracellular pathway regulation.

Rab 6A has connections with neurological conditions. Its dysfunction or misregulation can impact neurodegenerative diseases such as Alzheimer's disease by disrupting protein processing and transport. Rab 6A interacts with amyloid precursor protein-processing pathways emphasizing its role in disease pathways. Also Rab 6A is linked to disorders affecting the Golgi apparatus a critical site for protein post-translational modification and trafficking within cells.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulator of COPI-independent retrograde transport from the Golgi apparatus towards the endoplasmic reticulum (ER) (PubMed : 25962623). Has a low GTPase activity (PubMed : 25962623). Recruits VPS13B to the Golgi membrane (PubMed : 25492866). Plays a role in neuron projection development (Probable).
See full target information RAB6A

Additional targets

RALA,RAB6D
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com