AB86349

Anti-RAB antibody

Name: Anti-RAB antibody (ab86349) | Abcam
Brand: Abcam Limited
SKU: AB86349
Price: 520 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(2 Publications)

Rabbit Polyclonal RAB antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human AGFG1 aa 500 to C-terminus.

View Alternative Names

HRB, RAB, RIP, AGFG1, Arf-GAP domain and FG repeat-containing protein 1, HIV-1 Rev-binding protein, Nucleoporin-like protein RIP, Rev-interacting protein, Rev/Rex activation domain-binding protein

2 Images

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Western blot - Anti-RAB antibody (AB86349)

All lanes:

Western blot - Anti-RAB antibody (ab86349) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 58 kDa

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Exposure time: 10s

Unknown

Immunoprecipitation - Anti-RAB antibody (AB86349)

Detection of RAB by Western Blot of Immunprecipitate.
ab86349 at 1μg/ml staining RAB in HeLa whole cell lysate immunoprecipitated using ab86349 at 3μg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-RAB antibody (ab86349)

Predicted band size: 58 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human AGFG1 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P52594

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The RAB proteins also known as RAS-related proteins are small GTPases with a molecular weight of approximately 20–25 kDa. They belong to the Ras superfamily which are key regulators of intracellular trafficking. RAB proteins primarily localize to specific intracellular membranes where they direct vesicle movement docking and fusion. Different RAB proteins express in distinct tissues reflecting their specific roles in cell function. For example certain RAB proteins predominantly express in the brain while others are found in muscle or epithelial tissues.

Biological function summary

Small GTPases like RAB proteins operate as molecular switches in the cell. They participate in processes such as endocytosis exocytosis and other vesicle transport mechanisms. RAB proteins often work in conjunction with other members of the GTPase family and can assemble into regulatory complexes on vesicle membranes. These complexes control the specificity and timing of vesicle trafficking events ensuring precise delivery of cellular cargo.

Pathways

Small GTPases such as RAB proteins integrate into several key cellular signaling and trafficking pathways. They are vital components of the vesicular transport pathways including the endocytic and secretory pathways. RAB proteins communicate with effector proteins like the SNAREs to facilitate vesicle fusion and regulate membrane dynamics. Additionally they interact with components of the cytoskeleton to ensure vesicle movement within cells influencing both signaling and homeostatic pathways.

Aberrations in RAB proteins often link to neurological disorders and cancer. Mutations or dysregulation of specific RAB proteins can disrupt vesicle transport and lead to pathologies such as Charcot-Marie-Tooth disease or melanoma. In cancer changes in RAB function may alter signaling pathways that control cell proliferation and metastasis. Moreover RAB proteins can have interactions with oncogenes or neuroprotective proteins influencing disease progression and offering potential targets for therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for vesicle docking or fusion during acrosome biogenesis (By similarity). May play a role in RNA trafficking or localization. In case of infection by HIV-1, acts as a cofactor for viral Rev and promotes movement of Rev-responsive element-containing RNAs from the nuclear periphery to the cytoplasm. This step is essential for HIV-1 replication.

See full target information AGFG1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Bio-protocol 8:e3098 PubMed34532545

2018

Wheat Germ Agglutinin (WGA)-SDS-PAGE: A Novel Method for the Detection of O-GlcNAc-modified Proteins by Lectin Affinity Gel Electrophoresis.

Applications

Unspecified application

Species

Unspecified reactive species

Ko Fujioka,Yuji Kubota,Mutsuhiro Takekawa

PloS one 12:e0180714 PubMed28686627

2017

WGA-based lectin affinity gel electrophoresis: A novel method for the detection of O-GlcNAc-modified proteins.

Applications

Unspecified application

Species

Human

Yuji Kubota,Ko Fujioka,Mutsuhiro Takekawa

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RAB antibody

Western blot - Anti-RAB antibody (AB86349)

Immunoprecipitation - Anti-RAB antibody (AB86349)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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