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Rabbit Recombinant Monoclonal RAB10 antibody. Suitable for Flow Cyt (Intra), WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

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Images

Western blot - Anti-RAB10 antibody [EPR13242] (AB181367), expandable thumbnail
  • Western blot - Anti-RAB10 antibody [EPR13242] (AB181367), expandable thumbnail
  • Western blot - Anti-RAB10 antibody [EPR13242] (AB181367), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-RAB10 antibody [EPR13242] (AB181367), expandable thumbnail
  • Western blot - Anti-RAB10 antibody [EPR13242] (AB181367), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)ICC/IFIPWBIHC-P
Human
Tested
Not recommended
Not recommended
Tested
Not recommended
Mouse
Expected
Not recommended
Not recommended
Tested
Not recommended
Rat
Expected
Not recommended
Not recommended
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse
Dilution info
1/10 - 1/100
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species
Rat
Dilution info
1/10 - 1/100
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/1000 - 1/5000
Notes

-

Species
Mouse
Dilution info
1/1000 - 1/5000
Notes

-

Species
Human
Dilution info
1/1000 - 1/5000
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes (PubMed:21248164). Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (PubMed:21248164). That Rab is mainly involved in the biosynthetic transport of proteins from the Golgi to the plasma membrane (PubMed:21248164). Regulates, for instance, SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane (By similarity). In parallel, it regulates the transport of TLR4, a toll-like receptor to the plasma membrane and therefore may be important for innate immune response (By similarity). Also plays a specific role in asymmetric protein transport to the plasma membrane (PubMed:16641372). In neurons, it is involved in axonogenesis through regulation of vesicular membrane trafficking toward the axonal plasma membrane (By similarity). In epithelial cells, it regulates transport from the Golgi to the basolateral membrane (PubMed:16641372). May play a role in the basolateral recycling pathway and in phagosome maturation (By similarity). May play a role in endoplasmic reticulum dynamics and morphology controlling tubulation along microtubules and tubules fusion (PubMed:23263280). Together with LRRK2, RAB8A, and RILPL1, it regulates ciliogenesis (PubMed:30398148). When phosphorylated by LRRK2 on Thr-73, binds RILPL1 and inhibits ciliogenesis (PubMed:30398148). Participates in the export of a subset of neosynthesized proteins through a Rab8-Rab10-Rab11-dependent endososomal export route (PubMed:32344433). Targeted to and stabilized on stressed lysosomes through LRRK2 phosphorylation where it promotes the extracellular release of lysosomal content through EHBP1 and EHNP1L1 effector proteins (PubMed:30209220). (Microbial infection) Upon Legionella pneumophila infection promotes endoplasmic reticulum recruitment and bacterial replication. Plays a role in remodeling the Legionella-containing vacuole (LCV) into an endoplasmic reticulum-like vacuole.

Alternative names

Ras-related protein Rab-10, RAB10

Recommended products

Rabbit Recombinant Monoclonal RAB10 antibody. Suitable for Flow Cyt (Intra), WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR13242
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

RAB10 also known as RAB10 member RAS oncogene family is a small GTPase belonging to the RAB protein family. It has a molecular weight of approximately 24 kDa. RAB10 is ubiquitously expressed with higher levels observed in brain liver and lung tissues. It cycles between an active GTP-bound state and an inactive GDP-bound state regulating various membrane trafficking events within the cell. Its role extends to modulating the transport of proteins from the Golgi apparatus to the plasma membrane therefore influencing cellular logistics and function.

Biological function summary

RAB10 participates in vesicular trafficking and transport processes. It is part of the endosomal and exocytic complexes contributing to the sorting and delivery of cellular cargo. RAB10's activity affects the delivery of glucose transporter type 4 (GLUT4) to the plasma membrane impacting glucose uptake and metabolism. It also plays a critical role in the polarized transport of cargo in neuronal cells influencing neuronal signaling and function.

Pathways

RAB10 integrates into the insulin signaling and polarized epithelial cell transport pathways. Within these RAB10 cooperates with proteins like GLUT4 and the TBC1D4 a RAB GAP protein essential for GLUT4 vesicle translocation. These interactions ensure efficient response and coordination of cellular trafficking required for metabolic regulation and cellular growth processes.

Associated diseases and disorders

RAB10 shows connections to metabolic disorders and neurodegenerative diseases. Its role in glucose transporter trafficking links it to type 2 diabetes as improper GLUT4 trafficking disrupts glucose homeostasis. In the realm of neurodegenerative diseases proteins involved in neuronal transport and function such as APP and Tau may be influenced by RAB10-mediated pathways impacting diseases like Alzheimer's disease. The ability of RAB10 to interact with numerous cellular partners places it as a significant contributor to cellular dysfunction observed in these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-RAB10 antibody [EPR13242] (ab181367), expandable thumbnail

    Western blot - Anti-RAB10 antibody [EPR13242] (ab181367)

    Lanes 1 - 4: Merged signal (red and green). Green - ab181367 observed at 25 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    ab181367 was shown to specifically react with RAB10 in wild-type A549 cells as signal was lost in RAB10 knockout cell line Human RAB10 knockout A549 cell line ab261868 (knockout cell lysate Human RAB10 knockout A549 cell lysate ab261677). Wild-type and RAB10 knockout samples were subjected to SDS-PAGE. ab181367 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-RAB10 antibody [EPR13242] (ab181367) at 1/1000 dilution

    Lane 1: Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

    Lane 2: RAB10 knockout A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

    Lane 2: Western blot - Human RAB10 knockout A549 cell line (Human RAB10 knockout A549 cell line ab261868)

    Lane 3: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 4: MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 22 kDa

    Observed band size: 25 kDa

  • Western blot - Anti-RAB10 antibody [EPR13242] (ab181367), expandable thumbnail

    Western blot - Anti-RAB10 antibody [EPR13242] (ab181367)

    All lanes: Western blot - Anti-RAB10 antibody [EPR13242] (ab181367) at 1/1000 dilution

    Lane 1: U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates at 15 µg

    Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 22 kDa

    Observed band size: 22 kDa

  • Western blot - Anti-RAB10 antibody [EPR13242] (ab181367), expandable thumbnail

    Western blot - Anti-RAB10 antibody [EPR13242] (ab181367)

    All lanes: Western blot - Anti-RAB10 antibody [EPR13242] (ab181367) at 1/2000 dilution

    Lane 1: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 15 µg

    Lane 2: C6 (Rat glial tumor glial cell) whole cell lysates at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 22 kDa

    Observed band size: 22 kDa

  • Flow Cytometry (Intracellular) - Anti-RAB10 antibody [EPR13242] (ab181367), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-RAB10 antibody [EPR13242] (ab181367)

    Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling RAB10 with Purified ab181367 at 1/20 dilution (10 μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Western blot - Anti-RAB10 antibody [EPR13242] (ab181367), expandable thumbnail

    Western blot - Anti-RAB10 antibody [EPR13242] (ab181367)

    ab181367 was shown to react with RAB10 in wild-type HAP1 cells in Western blot with loss of signal observed in a RAB10 knockout cell line. Wild-type HAP1 and RAB10 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab181367 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

    These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

    All lanes: Western blot - Anti-RAB10 antibody [EPR13242] (ab181367) at 1/1000 dilution

    Lane 1: Wild-type HAP1 lysate at 40 µg

    Lane 2: RAB10 knock-out HAP1 lysate at 40 µg

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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