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AB241060

Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5]

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(23 Publications)

Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] (ab241060) is a rabbit monoclonal antibody detecting RAB10 in Western Blot, ICC/IF, Dot Blot. Suitable for Human, Mouse.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

Ras-related protein Rab-10, RAB10

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] (AB241060)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] (AB241060)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Saponin permeabilized MEF (mouse embryonic fibroblast) generated from wild-type or mutant LRRK2[R1441C] embryos without and with (+) MLi-2 treatment (100nM for 60 min) cells labeling RAB10 (phospho T73) with ab241060 at 0.5 μg/mL, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) secondary antibody at 1/1000 dilution (red). Nuclear counterstain is DAPI.

The image is kindly provided by our collaborator Dr. Dario Alessi, University of Dundee.

Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] (AB241060)
  • WB

Supplier Data

Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] (AB241060)

Blocking buffer : 5% NFDM/TBST.

Dilution buffer : 5% BSA/TBST.

MLi-2 is a potent, selective LARRK2 kinase inhibitor (PMID : 26407721).

The blot was scanned with LI-COR Odyssey CLx.

This image is kindly provided by our collaborator Dr. Dario Alessi, University of Dundee.

All lanes:

Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] (ab241060) at 1/500 dilution

Lane 1:

HEK293 (human embryonic kidney epithelial cells) overexpressing HA-tagged Rab3, whole cell lysate at 3 µg

Lane 2:

HEK293 overexpressing HA-tagged Rab3, with (+) MLi2 treatment (100nM for 60 min), whole cell lysate at 3 µg

Lane 3:

HEK293 overexpressing HA-tagged Rab5, whole cell lysate at 3 µg

Lane 4:

HEK293 overexpressing HA-tagged Rab5, with (+) MLi2 treatment (100nM for 60 min), whole cell lysate at 3 µg

Lane 5:

HEK293 overexpressing HA-tagged Rab8, whole cell lysate at 3 µg

Lane 6:

HEK293 overexpressing HA-tagged Rab8, with (+) MLi2 treatment (100nM for 60 min), whole cell lysate at 3 µg

Lane 7:

HEK293 overexpressing HA-tagged Rab10, whole cell lysate at 3 µg

Lane 8:

HEK293 overexpressing HA-tagged Rab10, with (+) MLi2 treatment (100nM for 60 min), whole cell lysate at 3 µg

Lane 9:

HEK293 overexpressing HA-tagged Rab12, whole cell lysate at 3 µg

Lane 10:

HEK293 overexpressing HA-tagged Rab12, with (+) MLi2 treatment (100nM for 60 min), whole cell lysate at 3 µg

Lane 11:

HEK293 overexpressing HA-tagged Rab29, whole cell lysate at 3 µg

Lane 12:

HEK293 overexpressing HA-tagged Rab29, with (+) MLi2 treatment (100nM for 60 min), whole cell lysate at 3 µg

Lane 13:

HEK293 overexpressing HA-tagged Rab35, whole cell lysate at 3 µg

Lane 14:

HEK293 overexpressing HA-tagged Rab35, with (+) MLi2 treatment (100nM for 60 min), whole cell lysate at 3 µg

Lane 15:

HEK293 overexpressing HA-tagged Rab43, whole cell lysate at 3 µg

Lane 16:

HEK293 overexpressing HA-tagged Rab43, with (+) MLi2 treatment (100nM for 60 min), whole cell lysate at 3 µg

Secondary

All lanes:

IRDye® 800CW Goat-anti-Rabbit at 1/25000 dilution

Predicted band size: 22 kDa

false

Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] (AB241060)
  • WB

Lab

Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] (AB241060)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Observed MW 28kDa

All lanes:

Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] (ab241060) at 1/200 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) transfected with an empty vector, containing a myc-His-tag®, whole cell lysate at 40 µg

Lane 2:

293T transfected with RAB10 expression vector containing a myc-His-tag®, whole cell lysate at 40 µg

Secondary

Lane 1:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Lane 2:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)

Predicted band size: 22 kDa

false

Exposure time: 60s

Dot Blot - Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] (AB241060)
  • Dot

Supplier Data

Dot Blot - Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] (AB241060)

Dot Blot - Anti-RAB10 antibody [MJF-R21-22-5] (ab241060) at a 1/1000 dilution.

Lane 1 : Rab10 (phospho T73) peptide

Lane 2 : Rab10 non-phospho peptide

Secondary antibody : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at a 1/100000 dilution.

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 180 seconds.

  • Carrier free

    Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

MJF-R21-22-5

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Synthetic peptide": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

What is this antibody validated in?
Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] (ab241060) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF), Dot Blot in Human, Mouse samples.

What is the molecular weight of RAB10?
Anti-RAB10 (phospho T73) [MJF-R21-22-5] (ab241060) specifically detects a band for RAB10 (UniProt: P61026) at a molecular weight of 22kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [MJF-R21-22-5] also available for your convenience: ab241060, Carrier free - ab243293, ab315172, Carrier free - ab315173

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Collaborations
This antibody was developed with support from The Michael J. Fox Foundation.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAB10 also known as RAB10 member RAS oncogene family is a small GTPase belonging to the RAB protein family. It has a molecular weight of approximately 24 kDa. RAB10 is ubiquitously expressed with higher levels observed in brain liver and lung tissues. It cycles between an active GTP-bound state and an inactive GDP-bound state regulating various membrane trafficking events within the cell. Its role extends to modulating the transport of proteins from the Golgi apparatus to the plasma membrane therefore influencing cellular logistics and function.
Biological function summary

RAB10 participates in vesicular trafficking and transport processes. It is part of the endosomal and exocytic complexes contributing to the sorting and delivery of cellular cargo. RAB10's activity affects the delivery of glucose transporter type 4 (GLUT4) to the plasma membrane impacting glucose uptake and metabolism. It also plays a critical role in the polarized transport of cargo in neuronal cells influencing neuronal signaling and function.

Pathways

RAB10 integrates into the insulin signaling and polarized epithelial cell transport pathways. Within these RAB10 cooperates with proteins like GLUT4 and the TBC1D4 a RAB GAP protein essential for GLUT4 vesicle translocation. These interactions ensure efficient response and coordination of cellular trafficking required for metabolic regulation and cellular growth processes.

RAB10 shows connections to metabolic disorders and neurodegenerative diseases. Its role in glucose transporter trafficking links it to type 2 diabetes as improper GLUT4 trafficking disrupts glucose homeostasis. In the realm of neurodegenerative diseases proteins involved in neuronal transport and function such as APP and Tau may be influenced by RAB10-mediated pathways impacting diseases like Alzheimer's disease. The ability of RAB10 to interact with numerous cellular partners places it as a significant contributor to cellular dysfunction observed in these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes (PubMed : 21248164). Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (PubMed : 21248164). That Rab is mainly involved in the biosynthetic transport of proteins from the Golgi to the plasma membrane (PubMed : 21248164). Regulates, for instance, SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane (By similarity). In parallel, it regulates the transport of TLR4, a toll-like receptor to the plasma membrane and therefore may be important for innate immune response (By similarity). Also plays a specific role in asymmetric protein transport to the plasma membrane (PubMed : 16641372). In neurons, it is involved in axonogenesis through regulation of vesicular membrane trafficking toward the axonal plasma membrane (By similarity). In epithelial cells, it regulates transport from the Golgi to the basolateral membrane (PubMed : 16641372). May play a role in the basolateral recycling pathway and in phagosome maturation (By similarity). May play a role in endoplasmic reticulum dynamics and morphology controlling tubulation along microtubules and tubules fusion (PubMed : 23263280). Together with LRRK2, RAB8A, and RILPL1, it regulates ciliogenesis (PubMed : 30398148). When phosphorylated by LRRK2 on Thr-73, binds RILPL1 and inhibits ciliogenesis (PubMed : 30398148). Participates in the export of a subset of neosynthesized proteins through a Rab8-Rab10-Rab11-dependent endososomal export route (PubMed : 32344433). Targeted to and stabilized on stressed lysosomes through LRRK2 phosphorylation where it promotes the extracellular release of lysosomal content through EHBP1 and EHNP1L1 effector proteins (PubMed : 30209220).. (Microbial infection) Upon Legionella pneumophila infection promotes endoplasmic reticulum recruitment and bacterial replication. Plays a role in remodeling the Legionella-containing vacuole (LCV) into an endoplasmic reticulum-like vacuole.
See full target information RAB10 phospho T73
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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

NPJ Parkinson's disease 11:267 PubMed40883285

2025

Lrrk2 G2019S mutation incites increased cell-intrinsic neutrophil effector functions and intestinal inflammation in a model of infectious colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica Pei,Nathalia L Oliveira,Sherilyn J Recinto,Alexandra Kazanova,Celso M Queiroz-Junior,Ziyi Li,Katalina Couto,Susan Westfall,Ahmed M Fahmy,Camila Tiefensee-Ribeiro,Irah L King,Austen J Milnerwood,Michel Desjardins,Ajitha Thanabalasuriar,Jo Anne Stratton,Samantha Gruenheid

Cells 14: PubMed40801573

2025

The Interaction of pT73-Rab10 with Myosin Va, but Not Myosin Vb, Is Regulated Though a Site in the Globular Tail Domain.

Applications

Unspecified application

Species

Unspecified reactive species

Lynne A Lapierre,Elizabeth H Manning,Kyra S Thomas,Catherine Caldwell,James R Goldenring

Nature communications 16:7226 PubMed40764514

2025

14-3-3 binding maintains the Parkinson's associated kinase LRRK2 in an inactive state.

Applications

Unspecified application

Species

Unspecified reactive species

Juliana A Martinez Fiesco,Alexandra Beilina,Astrid Alvarez de la Cruz,Ning Li,Riley D Metcalfe,Mark R Cookson,Ping Zhang

EMBO reports 26:3327-3356 PubMed40394349

2025

Inflammation and IL-4 regulate Parkinson's and Crohn's disease associated kinase LRRK2.

Applications

Unspecified application

Species

Unspecified reactive species

Dina Dikovskaya,Rebecca Pemberton,Matthew Taylor,Anna Tasegian,Purbasha Bhattacharya,Karolina Zeneviciute,Esther M Sammler,Andrew J M Howden,Dario R Alessi,Mahima Swamy

Neuromolecular medicine 27:42 PubMed40397198

2025

Protective Effect of the LRRK2 Kinase Inhibition in Human Fibroblasts Bearing the Genetic Variant GBA1 K198E: Implications for Parkinson's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Patricia Perez-Abshana,Miguel Mendivil-Perez,Carlos Velez-Pardo,Marlene Jimenez-Del-Rio

Communications biology 8:716 PubMed40341772

2025

Peripheral immune cell response to stimulation stratifies Parkinson's disease progression from prodromal to clinical stages.

Applications

Unspecified application

Species

Unspecified reactive species

Julian R Mark,Ann M Titus,Hannah A Staley,Stephan Alvarez,Savanna Mahn,Nikolaus R McFarland,Rebecca L Wallings,Malú Gámez Tansey

Proceedings of the National Academy of Sciences of the United States of America 122:e2415422122 PubMed39982740

2025

STING-induced noncanonical autophagy regulates endolysosomal homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Tuozhi Huang,Chenglong Sun,Fenghe Du,Zhijian J Chen

Nucleic acids research 53: PubMed39657788

2024

Novel BRCA1-PLK1-CIP2A axis orchestrates homologous recombination-mediated DNA repair to maintain chromosome integrity during oocyte meiosis.

Applications

Unspecified application

Species

Unspecified reactive species

Crystal Lee,Jeong Su Oh

Science immunology 9:eadi7907 PubMed39514635

2024

Macrophage LRRK2 hyperactivity impairs autophagy and induces Paneth cell dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Shengxiang Sun,Miki Hodel,Xiang Wang,Javier De Vicente,Talin Haritunians,Anketse Debebe,Chen-Ting Hung,Changqing Ma,Atika Malique,Hoang N Nguyen,Maayan Agam,Michael T Maloney,Marisa S Goo,Jillian H Kluss,Richa Mishra,Jennifer Frein,Amanda Foster,Samuel Ballentine,Uday Pandey,Justin Kern,Shaohong Yang,Emebet Mengesha,Iyshwarya Balasubramanian,Annie Arguello,Anthony A Estrada,Nan Gao,Inga Peter,Dermot P B McGovern,Anastasia G Henry,Thaddeus S Stappenbeck,Ta-Chiang Liu

Cell death & disease 15:752 PubMed39419978

2024

PAK6 rescues pathogenic LRRK2-mediated ciliogenesis and centrosomal cohesion defects in a mutation-specific manner.

Applications

Unspecified application

Species

Unspecified reactive species

Lucia Iannotta,Rachel Fasiczka,Giulia Favetta,Yibo Zhao,Elena Giusto,Elena Dall'Ara,Jianning Wei,Franz Y Ho,Claudia Ciriani,Susanna Cogo,Isabella Tessari,Ciro Iaccarino,Maxime Liberelle,Luigi Bubacco,Jean-Marc Taymans,Claudia Manzoni,Arjan Kortholt,Laura Civiero,Sabine Hilfiker,Michael L Lu,Elisa Greggio
View all publications
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Product promise

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