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Anti-RAB10 (phospho T73) antibody [MJF-R21] (ab230261) is a rabbit monoclonal antibody detecting RAB10 in Western Blot, Dot Blot. Suitable for Human, Mouse.



- KO validated for confirmed specificity

- Biophysical QC for unrivalled batch-batch consistency

- Over 60 publications

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Images

Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] (AB230261), expandable thumbnail
  • Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] (AB230261), expandable thumbnail
  • Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] (AB230261), expandable thumbnail
  • Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] (AB230261), expandable thumbnail
  • Dot Blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] (AB230261), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBDot
Human
Tested
Expected
Mouse
Tested
Expected
Synthetic peptide
Not recommended
Tested
Transfected cell lysate - Human
Tested
Not recommended

Tested
Tested

Species
Transfected cell lysate - Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Mouse, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

8 products for Alternative Product

Target data

Function

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes (PubMed:21248164). Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (PubMed:21248164). That Rab is mainly involved in the biosynthetic transport of proteins from the Golgi to the plasma membrane (PubMed:21248164). Regulates, for instance, SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane (By similarity). In parallel, it regulates the transport of TLR4, a toll-like receptor to the plasma membrane and therefore may be important for innate immune response (By similarity). Also plays a specific role in asymmetric protein transport to the plasma membrane (PubMed:16641372). In neurons, it is involved in axonogenesis through regulation of vesicular membrane trafficking toward the axonal plasma membrane (By similarity). In epithelial cells, it regulates transport from the Golgi to the basolateral membrane (PubMed:16641372). May play a role in the basolateral recycling pathway and in phagosome maturation (By similarity). May play a role in endoplasmic reticulum dynamics and morphology controlling tubulation along microtubules and tubules fusion (PubMed:23263280). Together with LRRK2, RAB8A, and RILPL1, it regulates ciliogenesis (PubMed:30398148). When phosphorylated by LRRK2 on Thr-73, binds RILPL1 and inhibits ciliogenesis (PubMed:30398148). Participates in the export of a subset of neosynthesized proteins through a Rab8-Rab10-Rab11-dependent endososomal export route (PubMed:32344433). Targeted to and stabilized on stressed lysosomes through LRRK2 phosphorylation where it promotes the extracellular release of lysosomal content through EHBP1 and EHNP1L1 effector proteins (PubMed:30209220). (Microbial infection) Upon Legionella pneumophila infection promotes endoplasmic reticulum recruitment and bacterial replication. Plays a role in remodeling the Legionella-containing vacuole (LCV) into an endoplasmic reticulum-like vacuole.

Alternative names

Ras-related protein Rab-10, RAB10

Recommended products

Anti-RAB10 (phospho T73) antibody [MJF-R21] (ab230261) is a rabbit monoclonal antibody detecting RAB10 in Western Blot, Dot Blot. Suitable for Human, Mouse.



- KO validated for confirmed specificity

- Biophysical QC for unrivalled batch-batch consistency

- Over 60 publications

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
MJF-R21
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-RAB10 (phospho T73) antibody [MJF-R21] (ab230261) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Dot Blot in Human, Mouse samples.

What is the molecular weight of RAB10?


Anti-RAB10 (phospho T73) [MJF-R21] (ab230261) specifically detects a band for RAB10 (UniProt: P61026) at a molecular weight of 23kDa.

Trusted by the scientific community


Anti-RAB10 (phospho T73) [MJF-R21] (ab230261) was first used in a scientific publication in 2018 and has been cited over 60 times in peer-reviewed journals.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed


The specificity of Anti-RAB10 (phospho T73) antibody [MJF-R21] (ab230261) has been confirmed by Western blot testing in RAB10 Knockout MEF cells.



Other related products


We have a range of other formats of antibody clone [MJF-R21] also available for your convenience:
ab230261, Carrier free - Anti-RAB10 (phospho T73) antibody [MJF-R21] - BSA and Azide free ab231707, Anti-RAB10 (phospho T73) antibody [MJF-R21] - Mouse IgG2a (Chimeric) ab315174, Carrier free - Anti-RAB10 (phospho T73) antibody [MJF-R21] - Mouse IgG2a (Chimeric) - BSA and Azide free ab315175



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Collaborations
This antibody was developed with support from The Michael J. Fox Foundation.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

RAB10 also known as RAB10 member RAS oncogene family is a small GTPase belonging to the RAB protein family. It has a molecular weight of approximately 24 kDa. RAB10 is ubiquitously expressed with higher levels observed in brain liver and lung tissues. It cycles between an active GTP-bound state and an inactive GDP-bound state regulating various membrane trafficking events within the cell. Its role extends to modulating the transport of proteins from the Golgi apparatus to the plasma membrane therefore influencing cellular logistics and function.

Biological function summary

RAB10 participates in vesicular trafficking and transport processes. It is part of the endosomal and exocytic complexes contributing to the sorting and delivery of cellular cargo. RAB10's activity affects the delivery of glucose transporter type 4 (GLUT4) to the plasma membrane impacting glucose uptake and metabolism. It also plays a critical role in the polarized transport of cargo in neuronal cells influencing neuronal signaling and function.

Pathways

RAB10 integrates into the insulin signaling and polarized epithelial cell transport pathways. Within these RAB10 cooperates with proteins like GLUT4 and the TBC1D4 a RAB GAP protein essential for GLUT4 vesicle translocation. These interactions ensure efficient response and coordination of cellular trafficking required for metabolic regulation and cellular growth processes.

Associated diseases and disorders

RAB10 shows connections to metabolic disorders and neurodegenerative diseases. Its role in glucose transporter trafficking links it to type 2 diabetes as improper GLUT4 trafficking disrupts glucose homeostasis. In the realm of neurodegenerative diseases proteins involved in neuronal transport and function such as APP and Tau may be influenced by RAB10-mediated pathways impacting diseases like Alzheimer's disease. The ability of RAB10 to interact with numerous cellular partners places it as a significant contributor to cellular dysfunction observed in these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] (ab230261), expandable thumbnail

    Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] (ab230261)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Observed MW 28 kDa

    All lanes: Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] (ab230261) at 1/1000 dilution

    Lane 1: 293T (Human embryonic kidney epithelial cell) transfected with an empty vector, containing a myc-His-tag®, whole cell lysate at 20 µg

    Lane 2: 293T transfected with RAB10 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 22 kDa

    Exposure time: 180s

  • Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] (ab230261), expandable thumbnail

    Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] (ab230261)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The LRRK2 pathogenic mutation R1441C increases LRRK2 activity and markedly elevates Rab10 phosphorylation in MEF (mouse embryonic fibroblasts).

    The expression pattern is consistent with the literature (PMID: 29127256).

    The cell lysates were kindly provided by our collaborator, Dr. Dario Alessi.

    All lanes: Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] (ab230261) at 1/1000 dilution

    Lane 1: Wild-type MEF (mouse embryonic fibroblast cell line) whole cell lysate at 20 µg

    Lane 2: Wild-type MEF treated with 100 nM MLi-2 for 90 minutes, whole cell lysate at 20 µg

    Lane 3: LRRK2 [R1441C] knock-in MEF whole cell lysate at 20 µg

    Lane 4: LRRK2 [R1441C] knock-in MEF treated with 100 nM MLi-2 for 90 minutes, whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 22 kDa

    Observed band size: 23 kDa

    Exposure time: 3min

  • Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] (ab230261), expandable thumbnail

    Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] (ab230261)

    Blocking buffer: 5% NFDM/TBST.

    Dilution buffer: 5% BSA/TBST.

    The images were kindly provided by our collaborator, Dr. Dario Alessi, and have been published (PMID: 29127256).

    Scanned with Licor Odyssey CLx.

    All lanes: Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] (ab230261) at 1/1000 dilution

    Lane 1: Wild-type A549 (human lung carcinoma cell line) whole cell lysate at 20 µg

    Lane 2: Wild-type A549 treated with 100 nM MLi-2 for 90 minutes, whole cell lysate at 20 µg

    Lane 3: Rab8A knock-out A549 whole cell lysate at 20 µg

    Lane 4: Rab8A knock-out A549 treated with 100 nM MLi-2 for 90 minutes, whole cell lysate at 20 µg

    Lane 5: Rab10 knock-out A549 whole cell lysate at 20 µg

    Lane 6: Rab10 knock-out A549 treated with 100 nM MLi-2 for 90 minutes, whole cell lysate at 20 µg

    Secondary

    All lanes: HRP-labeled secondary antibody at 1/2500 dilution

    Predicted band size: 22 kDa

    Observed band size: 23 kDa

  • Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] (ab230261), expandable thumbnail

    Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] (ab230261)

    Blocking buffer: 5% NFDM/TBST.

    Dilution buffer: 5% BSA/TBST.

    The LRRK2 pathogenic mutation Y1699C increases LRRK2 activity and markedly elevates the phosphorylation of Rab proteins.

    The images were kindly provided by our collaborator Dr. Dario Alessi, and have been published (PMID: 29127256).

    Scanned with Licor Odyssey CLx.

    All lanes: Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] (ab230261) at 1/1000 dilution

    Lane 1: HEK-293 (human epithelial cell line from embryonic kidney) cells transfected with LRRK2 [Y1699C] and HA-tagged Rab3A expression vectors, were treated with 150 nM MLi-2 for 90 minutes, whole at 0.1 µg

    Lane 2: HEK-293 cells transfected with LRRK2 [Y1699C] and HA-tagged Rab8A expression vectors, were treated with 150 nM MLi-2 for 90 minutes, whole cell lysate at 0.1 µg

    Lane 3: HEK-293 cells transfected with LRRK2 [Y1699C] and HA-tagged Rab10 expression vectors, were treated with 150 nM MLi-2 for 90 minutes, whole cell lysate at 0.1 µg

    Lane 4: HEK-293 cells transfected with LRRK2 [Y1699C] and HA-tagged Rab35 expression vectors, were treated with 150 nM MLi-2 for 90 minutes, whole cell lysate at 0.1 µg

    Lane 5: HEK-293 cells transfected with LRRK2[Y1699C] and HA-tagged Rab43 expression vectors, were treated with 150 nM MLi-2 for 90 minutes, whole cell lysate at 0.1 µg

    Secondary

    All lanes: IRDye 800CW secondary antibody at 1/25000 dilution

    Predicted band size: 22 kDa

    Observed band size: 23 kDa

  • Dot Blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] (ab230261), expandable thumbnail

    Dot Blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] (ab230261)

    Dot blot analysis of Rab10 (phospho T73) labeled with ab230261 at 1/1000 dilution.

    Lane 1: Rab10 (phospho T73) peptide;

    Lane 2: Rab10 non-phospho peptide.

    Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100,000 dilution was used as secondary antibody.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 32 seconds.

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Product protocols

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