AB232655

Anti-RAB11-FIP2 antibody [EPR12294-85] - BSA and Azide free

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Recombinant Monoclonal RAB11-FIP2 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

View Alternative Names

KIAA0941, RAB11FIP2, Rab11 family-interacting protein 2, Rab11-FIP2, NRip11

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-RAB11-FIP2 antibody [EPR12294-85] - BSA and Azide free (AB232655)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RAB11-FIP2 antibody [EPR12294-85] - BSA and Azide free (AB232655)

Immunofluorescence analysis of, paraformaldehyde-fixed, 293 cells labeling RAB11-FIP2 with unpurified
ab180504 at a 1/100 dilution. As secondary antibody goat anti rabbit IgG (Alexa Fluor®555) was used at a 1/250. In blue DAPI staining.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180504).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB11-FIP2 antibody [EPR12294-85] - BSA and Azide free (AB232655)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB11-FIP2 antibody [EPR12294-85] - BSA and Azide free (AB232655)

Immunohistochemical analysis of paraffin-embedded, Human brain tissue labeling RAB11-FIP2 with unpurified
ab180504 at a 1/250 dilution. Counter stained with hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180504).

Immunocytochemistry/ Immunofluorescence - Anti-RAB11-FIP2 antibody [EPR12294-85] - BSA and Azide free (AB232655)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-RAB11-FIP2 antibody [EPR12294-85] - BSA and Azide free (AB232655)

Immunocytochemistry/ Immunofluorescence analysis of HEK-293 (human embryonic kidney epithelial cell) cells labeling RAB11-FIP2 with purified ab180504 at 1 : 100 (5.5 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180504).

Flow Cytometry (Intracellular) - Anti-RAB11-FIP2 antibody [EPR12294-85] - BSA and Azide free (AB232655)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-RAB11-FIP2 antibody [EPR12294-85] - BSA and Azide free (AB232655)

Intracellular Flow Cytometry analysis of HEK-293 (Human embryonic kidney epithelial cell) cells labeling RAB11-FIP2 with purified ab180504 at 1/50 dilution (10 μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180504).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB11-FIP2 antibody [EPR12294-85] - BSA and Azide free (AB232655)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB11-FIP2 antibody [EPR12294-85] - BSA and Azide free (AB232655)

Immunohistochemical (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon cancer tissue sections labeling RAB11-FIP2 with Purified ab180504 at 1 : 500 dilution (1.09 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180504).

Unconjugated

Anti-RAB11-FIP2 antibody [EPR12294-85]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12294-85

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-P, Flow Cyt (Intra), ICC/IF, WB

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

style

left-column

Product details

ab232655 is the carrier-free version of ab180504.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAB11-FIP2 also known as Rab11 Family Interacting Protein 2 interacts mechanically with the small GTPase Rab11. It plays a role in the transport of recycling endosomes to the plasma membrane. The molecular mass of RAB11-FIP2 is approximately 55 kDa. This protein is expressed in a variety of tissues including epithelial cells and neurons indicating its role in diverse cellular environments. Its function is important for maintaining the dynamics of vesicular trafficking.

Biological function summary

The protein influences endocytic recycling processes facilitating the movement of molecules such as glucose transporters and integrins back to the cell surface. RAB11-FIP2 associates with several other proteins in the Rab11 pathway forming a complex that functions in the coordination and regulation of vesicular trafficking. Its precise regulation of membrane recycling has implications for processes like cell migration and signal transduction.

Pathways

The protein operates within the recycling endosome pathway and is part of the intricate network controlling intracellular trafficking. RAB11-FIP2 has significant interactions with proteins like Rab11 and EHD1. These interactions are integral to the proper functioning of pathways directing the distribution and localization of important cellular components impacting cellular homeostasis.

Improper regulation or mutations of RAB11-FIP2 have been associated with disorders such as cancer and epilepsy. Altered expression levels can affect cellular transport processes contributing to the progression of such diseases. Additionally RAB11-FIP2 shows connections with proteins like GLUT4 in metabolic pathways which suggests a possible role in metabolic disorders highlighting its clinical significance.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

A Rab11 effector binding preferentially phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) and phosphatidic acid (PA) and acting in the regulation of the transport of vesicles from the endosomal recycling compartment (ERC) to the plasma membrane. Involved in insulin granule exocytosis. Also involved in receptor-mediated endocytosis and membrane trafficking of recycling endosomes, probably originating from clathrin-coated vesicles. Required in a complex with MYO5B and RAB11 for the transport of NPC1L1 to the plasma membrane. Also acts as a regulator of cell polarity. Plays an essential role in phagocytosis through a mechanism involving TICAM2, RAC1 and CDC42 Rho GTPases for controlling actin-dynamics.

See full target information RAB11FIP2

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cells 13: PubMed39273021

2024

Cell-Sonar, a Novel Method for Intracellular Tracking of Secretory Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Sabrina Brockmöller,Thomas Seeger,Franz Worek,Simone Rothmiller

Cell reports 36:109491 PubMed34348154

2021

Exocyst protein subnetworks integrate Hippo and mTOR signaling to promote virus detection and cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Aubhishek Zaman,Xiaofeng Wu,Andrew Lemoff,Sivaramakrishna Yadavalli,Jeon Lee,Chensu Wang,Jonathan Cooper,Elizabeth A McMillan,Charles Yeaman,Hamid Mirzaei,Michael A White,Trever G Bivona

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com