AB128913

Anti-Rab11A antibody [EPR7587(B)]

Name: Anti-Rab11A antibody [EPR7587(B)] Rabbit monoclonal (ab128913) | Abcam
Brand: Abcam Limited
SKU: AB128913
Price: 210 USD
Availability: InStock
Rating: 5 (2 reviews)

20ul selling size
RabMAb
Recombinant
What is this?

(2 Reviews)

(22 Publications)

Rabbit Recombinant Monoclonal Rab11A antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 22 publications.

View Alternative Names

RAB11, RAB11A, Ras-related protein Rab-11A, Rab-11, YL8

5 Images

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Immunoprecipitation - Anti-Rab11A antibody [EPR7587(B)] (AB128913)

ab128913 (purified) at 1/20 immunoprecipitating Rab11A in A549 whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/10,000) was used for detection. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-Rab11A antibody [EPR7587(B)] (ab128913)

Predicted band size: 24 kDa

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Unknown

Western blot - Anti-Rab11A antibody [EPR7587(B)] (AB128913)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-Rab11A antibody [EPR7587(B)] (ab128913) at 1/10000 dilution

All lanes:

A549 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 24 kDa

false

Unknown

Western blot - Anti-Rab11A antibody [EPR7587(B)] (AB128913)

All lanes:

Western blot - Anti-Rab11A antibody [EPR7587(B)] (ab128913) at 1/10000 dilution

Lane 1:

Mouse brain cell lysate at 20 µg

Lane 2:

Mouse spleen lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 24 kDa

false

Unknown

Western blot - Anti-Rab11A antibody [EPR7587(B)] (AB128913)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-Rab11A antibody [EPR7587(B)] (ab128913) at 1/10000 dilution

Lane 1:

Rat brain lysate at 10 µg

Lane 2:

PC-12 cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 24 kDa

false

Unknown

Western blot - Anti-Rab11A antibody [EPR7587(B)] (AB128913)

All lanes:

Western blot - Anti-Rab11A antibody [EPR7587(B)] (ab128913) at 1/10000 dilution

Lane 1:

PC12 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

A549 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 109 kDa,142 kDa,17 kDa,24 kDa,27 kDa,274 kDa,29 kDa,39 kDa,40 kDa,44 kDa

Observed band size: 109 kDa,274 kDa,29 kDa

false

Carrier free

Anti-Rab11A antibody [EPR7587(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7587(B)

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rab11A also known as Ras-related protein Rab-11A is a member of the Rab family of small GTPases. This protein has a molecular weight of about 24.7 kDa and plays an important role in the regulation of intracellular recycling pathways. Rab11A is widely expressed in various tissues including the brain liver heart and lungs. It is found on recycling endosomes where it orchestrates vesicle trafficking a process critical for maintaining cellular homeostasis.

Biological function summary

Rab11A functions in intracellular transport by regulating vesicle budding and fusion. It ensures the proper recycling of membrane proteins like transferrin receptors and integrins. Rab11A interacts with a set of effector proteins that guide vesicle movement towards specific locations within the cell. This protein can also form complexes with other proteins such as Rab11FIP1 to facilitate effective cargo delivery. The actions of Rab11A are critical in processes like cell migration and growth factor signaling.

Pathways

Rab11A plays a role in the endocytic recycling and exocytic pathways. It ensures proper trafficking from endosomes to the plasma membrane. Rab11A interacts closely with proteins like Rab5 and Rab7 setting up a sequential transport system that regulates cargo delivery between different organelles. In the recycling pathway Rab11A is important for the sorting and return of cell surface receptors to the plasma membrane helping to maintain cell surface receptor pools.

Rab11A links to cancer and neurodegenerative diseases. Altered Rab11A expression and activity can affect processes like cell proliferation and apoptosis contributing to tumorigenesis. In neurodegenerative diseases aberrant Rab11A-mediated recycling may impact neurotrophic signaling and axonal transport leading to neuronal dysfunction. Mutations or dysregulation in related proteins such as Rab11FIP further implicate Rab11A in these diseases as they can disrupt the normal physiological functions governed by Rab11A.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (PubMed : 15601896, PubMed : 15689490, PubMed : 17462998, PubMed : 19542231, PubMed : 20026645, PubMed : 20890297, PubMed : 21282656, PubMed : 26032412). RAB11A regulates endocytic recycling (PubMed : 20026645). Forms a functional Rab11/RAB11FIP3/dynein complex that regulates the movement of peripheral sorting endosomes (SE) along microtubule tracks toward the microtubule organizing center/centrosome, generating the endosomal recycling compartment (ERC) (PubMed : 20026645). Acts as a major regulator of membrane delivery during cytokinesis (PubMed : 15601896). Together with MYO5B and RAB8A participates in epithelial cell polarization (PubMed : 21282656). Together with Rabin8/RAB3IP, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis (PubMed : 20890297). Together with MYO5B participates in CFTR trafficking to the plasma membrane and TF (Transferrin) recycling in nonpolarized cells (PubMed : 17462998). Required in a complex with MYO5B and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane (PubMed : 19542231). Participates in the sorting and basolateral transport of CDH1 from the Golgi apparatus to the plasma membrane (PubMed : 15689490). Regulates the recycling of FCGRT (receptor of Fc region of monomeric IgG) to basolateral membranes (By similarity). May also play a role in melanosome transport and release from melanocytes (By similarity). Promotes Rabin8/RAB3IP preciliary vesicular trafficking to mother centriole by forming a ciliary targeting complex containing Rab11, ASAP1, Rabin8/RAB3IP, RAB11FIP3 and ARF4, thereby regulating ciliogenesis initiation (PubMed : 25673879, PubMed : 31204173). On the contrary, upon LPAR1 receptor signaling pathway activation, interaction with phosphorylated WDR44 prevents Rab11-RAB3IP-RAB11FIP3 complex formation and cilia growth (PubMed : 31204173). Participates in the export of a subset of neosynthesized proteins through a Rab8-Rab10-Rab11-endososomal dependent export route via interaction with WDR44 (PubMed : 32344433). Also interacts with RABL3 to promote ciliary vesicle formation (PubMed : 36052645).

See full target information RAB11A

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Publications (22)

Recent publications for all applications. Explore the full list and refine your search

Cell & bioscience 15:69 PubMed40437641

2025

Rab11a-dependent recycling of Glut3 inhibits seizure-induced neuronal disulfidptosis by alleviating glucose deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Sijun Li,Junrui He,Huimin Kuang,Xiaojuan Wang,Muhua Zhou,Dongmei Li,Baoren Kang,Honghu He,Lina He,Wei Lin,Yuan Lv

iScience 28:112371 PubMed40330883

2025

Roles of class III phosphatidylinositol 3-kinase, Vps34, in phagocytosis, autophagy, and endocytosis in retinal pigmented epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Feng He,Ralph M Nichols,Melina A Agosto,Theodore G Wensel

The Journal of biological chemistry 300:107783 PubMed39303913

2024

Downregulation of the mA reader YTHDC2 upregulates exosome content in lung adenocarcinoma via inhibiting IFIT and OAS family members.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixin Yin,Lifang Ma,Xiaoting Tian,Qi Sun,Congcong Zhang,Yikun Wang,Yayou Miao,Xiangfei Xue,Yongjie Wang,Jiayi Wang,Xiao Zhang,Xumin Hou

eLife 12: PubMed37737843

2023

CRB3 navigates Rab11 trafficking vesicles to promote γTuRC assembly during ciliogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Wang,Zheyong Liang,Tan Tan,Miao Zhang,Yina Jiang,Yangyang Shang,Xiaoqian Gao,Shaoran Song,Ruiqi Wang,He Chen,Jie Liu,Juan Li,Yu Ren,Peijun Liu

Pharmaceutics 15: PubMed36986603

2023

Exploring Pathway to Hinder Chronic Myeloid Leukemia-Induced Angiogenesis In Vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Catarina Roma-Rodrigues,Alexandra R Fernandes,Pedro V Baptista

Cell genomics 3:100250 PubMed36950384

2023

Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Greta Pintacuda,Yu-Han H Hsu,Kalliopi Tsafou,Ka Wan Li,Jacqueline M Martín,Jackson Riseman,Julia C Biagini,Joshua K T Ching,Daya Mena,Miguel A Gonzalez-Lozano,Shawn B Egri,Jake Jaffe,August B Smit,Nadine Fornelos,Kevin C Eggan,Kasper Lage

Cellular and molecular life sciences : CMLS 79:615 PubMed36460928

2022

The endocytic recycling compartment serves as a viral factory for hepatitis E virus.

Applications

Unspecified application

Species

Unspecified reactive species

Cyrine Bentaleb,Kévin Hervouet,Claire Montpellier,Charline Camuzet,Martin Ferrié,Julien Burlaud-Gaillard,Stéphane Bressanelli,Karoline Metzger,Elisabeth Werkmeister,Maliki Ankavay,Nancy Leon Janampa,Julien Marlet,Julien Roux,Clarence Deffaud,Anne Goffard,Yves Rouillé,Jean Dubuisson,Philippe Roingeard,Cécile-Marie Aliouat-Denis,Laurence Cocquerel

Cellular and molecular gastroenterology and hepatology 14:295-310 PubMed35421597

2022

A Functional Relationship Between UNC45A and MYO5B Connects Two Rare Diseases With Shared Enteropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Qinghong Li,Zhe Zhou,Yue Sun,Chang Sun,Karin Klappe,Sven C D van IJzendoorn

eLife 11: PubMed35040777

2022

Lipid kinases VPS34 and PIKfyve coordinate a phosphoinositide cascade to regulate retriever-mediated recycling on endosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Sai Srinivas Panapakkam Giridharan,Guangming Luo,Pilar Rivero-Rios,Noah Steinfeld,Helene Tronchere,Amika Singla,Ezra Burstein,Daniel D Billadeau,Michael A Sutton,Lois S Weisman

Science advances 7:eabj8156 PubMed34860542

2021

MON-2, a Golgi protein, mediates autophagy-dependent longevity in .

Applications

Unspecified application

Species

Unspecified reactive species

Yoonji Jung,Murat Artan,Nari Kim,Jeonghun Yeom,Ara B Hwang,Dae-Eun Jeong,Özlem Altintas,Keunhee Seo,Mihwa Seo,Dongyeop Lee,Wooseon Hwang,Yujin Lee,Jooyeon Sohn,Eun Ji E Kim,Sungeun Ju,Seong Kyu Han,Hyun-Jun Nam,Linnea Adams,Youngjae Ryu,Dong Jin Moon,Chanhee Kang,Joo-Yeon Yoo,Sang Ki Park,Chang Man Ha,Malene Hansen,Sanguk Kim,Cheolju Lee,Seung-Yeol Park,Seung-Jae V Lee

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Rab11A antibody [EPR7587(B)]

Immunoprecipitation - Anti-Rab11A antibody [EPR7587(B)] (AB128913)

Western blot - Anti-Rab11A antibody [EPR7587(B)] (AB128913)

Western blot - Anti-Rab11A antibody [EPR7587(B)] (AB128913)

Western blot - Anti-Rab11A antibody [EPR7587(B)] (AB128913)

Western blot - Anti-Rab11A antibody [EPR7587(B)] (AB128913)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (22)

Product promise