Rabbit Polyclonal RAB11B antibody. Suitable for ICC, WB and reacts with Human, Dog samples. Cited in 36 publications. Immunogen corresponding to Synthetic Peptide within Human RAB11B aa 1-50.
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- Journal of virology 97:e00210232023Porcine Enteric Alphacoronavirus Entry through Multiple Pathways (Caveolae, Clathrin, and Macropinocytosis) Requires Rab GTPases for Endosomal Transport.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiong-Nan Chen,Yi-Fan Liang,Zhi-Jun Weng,Wei-Peng Quan,Chen Hu,Yun-Zhao Peng,Ying-Shuo Sun,Qi Gao,Zhao Huang,Gui-Hong Zhang,Lang GongPubMed 36975780
- Cellular and molecular gastroenterology and hepatology 15:533-5582022Hepatitis B Virus Utilizes a Retrograde Trafficking Route via the Trans-Golgi Network to Avoid Lysosomal Degradation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing-Yi Li,Kazuyuki Kuroki,Tetsuro Shimakami,Kazuhisa Murai,Kazunori Kawaguchi,Takayoshi Shirasaki,Kouki Nio,Saiho Sugimoto,Tomoki Nishikawa,Hikari Okada,Noriaki Orita,Hideo Takayama,Ying Wang,Phuong Doan Thi Bich,Astuya Ishida,Sadahiro Iwabuchi,Shinichi Hashimoto,Takeshi Shimaoka,Noriko Tabata,Miho Watanabe-Takahashi,Kiyotaka Nishikawa,Hiroshi Yanagawa,Motoharu Seiki,Kouji Matsushima,Taro Yamashita,Shuichi Kaneko,Masao HondaPubMed 36270602
- International journal of molecular sciences 23:2022SPIN90 Deficiency Ameliorates Amyloid β Accumulation by Regulating APP Trafficking in AD Model Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoungsoo Oh,Wongyoung Lee,So Hee Kim,Sooji Lee,Byeong C Kim,Kun Ho Lee,Sung Hyun Kim,Woo Keun SongPubMed 36142484
- Nature communications 13:46202022FERARI and cargo adaptors coordinate cargo flow through sorting endosomes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJachen A Solinger,Harun-Or Rashid,Anne SpangPubMed 35941155
- Nature communications 12:71292021Extracellular matrix remodeling through endocytosis and resurfacing of Tenascin-R.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTal M Dankovich,Rahul Kaushik,Linda H M Olsthoorn,Gabriel Cassinelli Petersen,Philipp Emanuel Giro,Verena Kluever,Paola Agüi-Gonzalez,Katharina Grewe,Guobin Bao,Sabine Beuermann,Hannah Abdul Hadi,Jose Doeren,Simon Klöppner,Benjamin H Cooper,Alexander Dityatev,Silvio O RizzoliPubMed 34880248
- Cancers 13:2021HMGB1 Protein Interactions in Prostate and Ovary Cancer Models Reveal Links to RNA Processing and Ribosome Biogenesis through NuRD, THOC and Septin Complexes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAida Barreiro-Alonso,Mónica Lamas-Maceiras,Lidia Lorenzo-Catoira,Mercedes Pardo,Lu Yu,Jyoti S Choudhary,M Esperanza CerdánPubMed 34572914
- Nature immunology 22:497-5092021TAP dysfunction in dendritic cells enables noncanonical cross-presentation for T cell priming.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGaëtan Barbet,Priyanka Nair-Gupta,Michael Schotsaert,Stephen T Yeung,Julien Moretti,Fabian Seyffer,Giorgi Metreveli,Thomas Gardner,Angela Choi,Domenico Tortorella,Robert Tampé,Kamal M Khanna,Adolfo García-Sastre,J Magarian BlanderPubMed 33790474
- Asian journal of pharmaceutical sciences 16:120-1282021Unraveling GLUT-mediated transcytosis pathway of glycosylated nanodisks.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuan Wang,Zui Zhang,Juan Guan,Weiyue Lu,Changyou ZhanPubMed 33613735
- Nature communications 11:30172020Rab11b-mediated integrin recycling promotes brain metastatic adaptation and outgrowth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesErin N Howe et. al.PubMed 32541798
- The Journal of cell biology 219:2020Nucleobindin-1 regulates ECM degradation by promoting intra-Golgi trafficking of MMPs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNatalia Pacheco-Fernandez,Mehrshad Pakdel,Birgit Blank,Ismael Sanchez-Gonzalez,Kathrin Weber,Mai Ly Tran,Tobias Karl-Heinz Hecht,Renate Gautsch,Gisela Beck,Franck Perez,Angelika Hausser,Stefan Linder,Julia von BlumePubMed 32479594
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human RAB11B aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q15907
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Reactivity data
Select an application| ICC | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Chicken | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Expected | Tested |
| Pig | Predicted | Predicted |
| Primates | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Dog | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Chicken, Cow, Pig, Primates | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes By Western blot, this antibody detects an ~22 kDa protein representing Rab 11 in CV1, PC3, and HeLa cell extract. Another less prominent, unknown band is also detected by this antibody at ~35 kDa. |
Species Dog | Dilution info - | Notes By Western blot, this antibody detects an ~22 kDa protein representing Rab 11 in CV1, PC3, and HeLa cell extract. Another less prominent, unknown band is also detected by this antibody at ~35 kDa. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Chicken, Cow, Pig, Primates | Dilution info - | Notes - |
Associated Products
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Target data
Function
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. The small Rab GTPase RAB11B plays a role in endocytic recycling, regulating apical recycling of several transmembrane proteins including cystic fibrosis transmembrane conductance regulator/CFTR, epithelial sodium channel/ENaC, potassium voltage-gated channel, and voltage-dependent L-type calcium channel. May also regulate constitutive and regulated secretion, like insulin granule exocytosis. Required for melanosome transport and release from melanocytes. Also regulates V-ATPase intracellular transport in response to extracellular acidosis. Promotes Rabin8/RAB3IP preciliary vesicular trafficking to mother centriole by forming a ciliary targeting complex containing Rab11, ASAP1, Rabin8/RAB3IP, RAB11FIP3 and ARF4, thereby regulating ciliogenesis initiation (Probable). On the contrary, upon LPAR1 receptor signaling pathway activation, interaction with phosphorylated WDR44 prevents Rab11-RAB3IP-RAB11FIP3 complex formation and cilia growth (Probable).
Alternative names
YPT3, RAB11B, Ras-related protein Rab-11B, GTP-binding protein YPT3
Recommended products
Rabbit Polyclonal RAB11B antibody. Suitable for ICC, WB and reacts with Human, Dog samples. Cited in 36 publications. Immunogen corresponding to Synthetic Peptide within Human RAB11B aa 1-50.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human RAB11B aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q15907
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RAB11B also known as Ras-related protein Rab-11B is a small GTPase enzyme that belongs to the RAB protein family. RAB11B has a molecular mass of approximately 24 kDa. This protein is expressed in several tissues particularly in brain and testes. It plays a critical role in the regulation of membrane trafficking. As a member of the RAB family RAB11B cycles between an inactive GDP-bound form and an active GTP-bound form which is necessary for its function in vesicular transport.
- Biological function summary
Small GTPases like RAB11B govern intracellular vesicle trafficking between different organelles. It resides mainly in recycling endosomes and is important for the recycling of receptors and other cell surface proteins. RAB11B often associates with other proteins like recycling endosome coat proteins to form complexes critical for its function. It maintains the proper localization and recycling of surface proteins which is essential for maintaining cellular function.
- Pathways
Regulation of vesicular transport by RAB11B integrates into several cellular pathways particularly the endocytic recycling pathway. This pathway supports the recycling of endocytosed material back to the plasma membrane. RAB11B interacts with other proteins such as RAB11A which has overlapping roles in vesicular transport to fine-tune the trafficking of molecules and maintain homeostasis.
- Associated diseases and disorders
RAB11B has links to conditions such as cancer and neurodegenerative diseases. Its dysregulation can result in abnormalities in vesicular trafficking which disrupts cellular homeostasis. For example in cancer altered RAB11B activity changes receptor recycling influencing cellular proliferation. In neurodegenerative diseases it may interact with amyloid precursor protein (APP) and facilitate events leading to disease progression. Changes in RAB11B function can associate with altered cell surface protein dynamics impacting disease development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunocytochemistry - Anti-RAB11B antibody (ab3612)
Immunocytochemical analysis of 4% paraformaldeehyde-fixed 0.1% Triton X-100 permeabilized HeLa cells treated/untreated with actinomycin D. Rab11B is stained with ab3612 at a 1/100 dilution and a Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor Plus 488 conjugate at a 1/500 dilution (green). Nuclear counterstaining is using Hoechst 33342 (blue).
Western blot - Anti-RAB11B antibody (ab3612)
All lanes: Western blot - Anti-RAB11B antibody (ab3612) at 1/500 dilution
All lanes: MDCK (canine kidney cell line) whole cell lysate at 20 µg
Predicted band size: 24 kDa
Western blot - Anti-RAB11B antibody (ab3612)
ab3612 used on PC3 cell extract.
All lanes: Western blot - Anti-RAB11B antibody (ab3612)
Predicted band size: 24 kDa
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