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AB3612

Anti-RAB11B antibody

4

(8 Reviews)

|

(38 Publications)

Rabbit Polyclonal RAB11B antibody. Suitable for ICC, WB and reacts with Human, Dog samples. Cited in 38 publications. Immunogen corresponding to Synthetic Peptide within Human RAB11B aa 1-50.

View Alternative Names

YPT3, RAB11B, Ras-related protein Rab-11B, GTP-binding protein YPT3

3 Images
Immunocytochemistry - Anti-RAB11B antibody (AB3612)
  • ICC

Supplier Data

Immunocytochemistry - Anti-RAB11B antibody (AB3612)

Immunocytochemical analysis of 4% paraformaldeehyde-fixed 0.1% Triton X-100 permeabilized HeLa cells treated/untreated with actinomycin D. Rab11B is stained with ab3612 at a 1/100 dilution and a Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor Plus 488 conjugate at a 1/500 dilution (green). Nuclear counterstaining is using Hoechst 33342 (blue).

Western blot - Anti-RAB11B antibody (AB3612)
  • WB

Unknown

Western blot - Anti-RAB11B antibody (AB3612)

ab3612 used on PC3 cell extract.

All lanes:

Western blot - Anti-RAB11B antibody (ab3612)

Predicted band size: 24 kDa

false

Western blot - Anti-RAB11B antibody (AB3612)
  • WB

Supplier Data

Western blot - Anti-RAB11B antibody (AB3612)

All lanes:

Western blot - Anti-RAB11B antibody (ab3612) at 1/500 dilution

All lanes:

MDCK (canine kidney cell line) whole cell lysate at 20 µg

Predicted band size: 24 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Dog, Human

Applications

ICC, WB

applications

Immunogen

Synthetic Peptide within Human RAB11B aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q15907

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>By Western blot, this antibody detects an ~22 kDa protein representing Rab 11 in CV1, PC3, and HeLa cell extract. Another less prominent, unknown band is also detected by this antibody at ~35 kDa.</p>" }, "Mouse": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>By Western blot, this antibody detects an ~22 kDa protein representing Rab 11 in CV1, PC3, and HeLa cell extract. Another less prominent, unknown band is also detected by this antibody at ~35 kDa.</p>" }, "Pig": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Primates": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAB11B also known as Ras-related protein Rab-11B is a small GTPase enzyme that belongs to the RAB protein family. RAB11B has a molecular mass of approximately 24 kDa. This protein is expressed in several tissues particularly in brain and testes. It plays a critical role in the regulation of membrane trafficking. As a member of the RAB family RAB11B cycles between an inactive GDP-bound form and an active GTP-bound form which is necessary for its function in vesicular transport.
Biological function summary

Small GTPases like RAB11B govern intracellular vesicle trafficking between different organelles. It resides mainly in recycling endosomes and is important for the recycling of receptors and other cell surface proteins. RAB11B often associates with other proteins like recycling endosome coat proteins to form complexes critical for its function. It maintains the proper localization and recycling of surface proteins which is essential for maintaining cellular function.

Pathways

Regulation of vesicular transport by RAB11B integrates into several cellular pathways particularly the endocytic recycling pathway. This pathway supports the recycling of endocytosed material back to the plasma membrane. RAB11B interacts with other proteins such as RAB11A which has overlapping roles in vesicular transport to fine-tune the trafficking of molecules and maintain homeostasis.

RAB11B has links to conditions such as cancer and neurodegenerative diseases. Its dysregulation can result in abnormalities in vesicular trafficking which disrupts cellular homeostasis. For example in cancer altered RAB11B activity changes receptor recycling influencing cellular proliferation. In neurodegenerative diseases it may interact with amyloid precursor protein (APP) and facilitate events leading to disease progression. Changes in RAB11B function can associate with altered cell surface protein dynamics impacting disease development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. The small Rab GTPase RAB11B plays a role in endocytic recycling, regulating apical recycling of several transmembrane proteins including cystic fibrosis transmembrane conductance regulator/CFTR, epithelial sodium channel/ENaC, potassium voltage-gated channel, and voltage-dependent L-type calcium channel. May also regulate constitutive and regulated secretion, like insulin granule exocytosis. Required for melanosome transport and release from melanocytes. Also regulates V-ATPase intracellular transport in response to extracellular acidosis. Promotes Rabin8/RAB3IP preciliary vesicular trafficking to mother centriole by forming a ciliary targeting complex containing Rab11, ASAP1, Rabin8/RAB3IP, RAB11FIP3 and ARF4, thereby regulating ciliogenesis initiation (Probable). On the contrary, upon LPAR1 receptor signaling pathway activation, interaction with phosphorylated WDR44 prevents Rab11-RAB3IP-RAB11FIP3 complex formation and cilia growth (Probable).
See full target information RAB11B
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Publications (38)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 97:e0021023 PubMed36975780

2023

Porcine Enteric Alphacoronavirus Entry through Multiple Pathways (Caveolae, Clathrin, and Macropinocytosis) Requires Rab GTPases for Endosomal Transport.

Applications

Unspecified application

Species

Unspecified reactive species

Xiong-Nan Chen,Yi-Fan Liang,Zhi-Jun Weng,Wei-Peng Quan,Chen Hu,Yun-Zhao Peng,Ying-Shuo Sun,Qi Gao,Zhao Huang,Gui-Hong Zhang,Lang Gong

Cellular and molecular gastroenterology and hepatology 15:533-558 PubMed36270602

2022

Hepatitis B Virus Utilizes a Retrograde Trafficking Route via the Trans-Golgi Network to Avoid Lysosomal Degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Ying-Yi Li,Kazuyuki Kuroki,Tetsuro Shimakami,Kazuhisa Murai,Kazunori Kawaguchi,Takayoshi Shirasaki,Kouki Nio,Saiho Sugimoto,Tomoki Nishikawa,Hikari Okada,Noriaki Orita,Hideo Takayama,Ying Wang,Phuong Doan Thi Bich,Astuya Ishida,Sadahiro Iwabuchi,Shinichi Hashimoto,Takeshi Shimaoka,Noriko Tabata,Miho Watanabe-Takahashi,Kiyotaka Nishikawa,Hiroshi Yanagawa,Motoharu Seiki,Kouji Matsushima,Taro Yamashita,Shuichi Kaneko,Masao Honda

International journal of molecular sciences 23: PubMed36142484

2022

SPIN90 Deficiency Ameliorates Amyloid β Accumulation by Regulating APP Trafficking in AD Model Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Youngsoo Oh,Wongyoung Lee,So Hee Kim,Sooji Lee,Byeong C Kim,Kun Ho Lee,Sung Hyun Kim,Woo Keun Song

Nature communications 13:4620 PubMed35941155

2022

FERARI and cargo adaptors coordinate cargo flow through sorting endosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Jachen A Solinger,Harun-Or Rashid,Anne Spang

Nature communications 12:7129 PubMed34880248

2021

Extracellular matrix remodeling through endocytosis and resurfacing of Tenascin-R.

Applications

Unspecified application

Species

Unspecified reactive species

Tal M Dankovich,Rahul Kaushik,Linda H M Olsthoorn,Gabriel Cassinelli Petersen,Philipp Emanuel Giro,Verena Kluever,Paola Agüi-Gonzalez,Katharina Grewe,Guobin Bao,Sabine Beuermann,Hannah Abdul Hadi,Jose Doeren,Simon Klöppner,Benjamin H Cooper,Alexander Dityatev,Silvio O Rizzoli

Cancers 13: PubMed34572914

2021

HMGB1 Protein Interactions in Prostate and Ovary Cancer Models Reveal Links to RNA Processing and Ribosome Biogenesis through NuRD, THOC and Septin Complexes.

Applications

Unspecified application

Species

Unspecified reactive species

Aida Barreiro-Alonso,Mónica Lamas-Maceiras,Lidia Lorenzo-Catoira,Mercedes Pardo,Lu Yu,Jyoti S Choudhary,M Esperanza Cerdán

Acta neuropathologica communications 9:109 PubMed34120654

2021

Autophagy is affected in patients with hypokalemic periodic paralysis: an involvement in vacuolar myopathy?

Applications

Unspecified application

Species

Unspecified reactive species

Thomas O Krag,Sonja Holm-Yildiz,Nanna Witting,John Vissing

Nature immunology 22:497-509 PubMed33790474

2021

TAP dysfunction in dendritic cells enables noncanonical cross-presentation for T cell priming.

Applications

Unspecified application

Species

Unspecified reactive species

Gaëtan Barbet,Priyanka Nair-Gupta,Michael Schotsaert,Stephen T Yeung,Julien Moretti,Fabian Seyffer,Giorgi Metreveli,Thomas Gardner,Angela Choi,Domenico Tortorella,Robert Tampé,Kamal M Khanna,Adolfo García-Sastre,J Magarian Blander

Asian journal of pharmaceutical sciences 16:120-128 PubMed33613735

2021

Unraveling GLUT-mediated transcytosis pathway of glycosylated nanodisks.

Applications

Unspecified application

Species

Unspecified reactive species

Huan Wang,Zui Zhang,Juan Guan,Weiyue Lu,Changyou Zhan

Nature communications 11:3017 PubMed32541798

2020

Rab11b-mediated integrin recycling promotes brain metastatic adaptation and outgrowth.

Applications

Unspecified application

Species

Unspecified reactive species

Erin N Howe,Miranda D Burnette,Melanie E Justice,Patricia M Schnepp,Victoria Hedrick,James W Clancy,Ian H Guldner,Alicia T Lamere,Jun Li,Uma K Aryal,Crislyn D'Souza-Schorey,Jeremiah J Zartman,Siyuan Zhang
View all publications
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