JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB175925

Anti-RAB11B antibody [EPR12725]

5

(1 Review)

|

(3 Publications)

Rabbit Recombinant Monoclonal RAB11B antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.

View Alternative Names

YPT3, RAB11B, Ras-related protein Rab-11B, GTP-binding protein YPT3

3 Images
Flow Cytometry (Intracellular) - Anti-RAB11B antibody [EPR12725] (AB175925)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-RAB11B antibody [EPR12725] (AB175925)

Intracellular flow cytometric analysis of Jurkat cells labeling RAB11B with ab175925 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

Immunoprecipitation - Anti-RAB11B antibody [EPR12725] (AB175925)
  • IP

Supplier Data

Immunoprecipitation - Anti-RAB11B antibody [EPR12725] (AB175925)

Western blot analysis on immunoprecipitation pellet from (1) fetal brain lysate or (2) 1X PBS (negative control) using ab175925 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-RAB11B antibody [EPR12725] (ab175925)

Predicted band size: 24 kDa

false

Western blot - Anti-RAB11B antibody [EPR12725] (AB175925)
  • WB

Supplier Data

Western blot - Anti-RAB11B antibody [EPR12725] (AB175925)

All lanes:

Western blot - Anti-RAB11B antibody [EPR12725] (ab175925) at 1/1000 dilution

Lane 1:

Fetal brain tissue lysate at 10 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 3:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Predicted band size: 24 kDa

false

  • Carrier free

    Anti-RAB11B antibody [EPR12725] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12725

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAB11B also known as Ras-related protein Rab-11B is a small GTPase enzyme that belongs to the RAB protein family. RAB11B has a molecular mass of approximately 24 kDa. This protein is expressed in several tissues particularly in brain and testes. It plays a critical role in the regulation of membrane trafficking. As a member of the RAB family RAB11B cycles between an inactive GDP-bound form and an active GTP-bound form which is necessary for its function in vesicular transport.
Biological function summary

Small GTPases like RAB11B govern intracellular vesicle trafficking between different organelles. It resides mainly in recycling endosomes and is important for the recycling of receptors and other cell surface proteins. RAB11B often associates with other proteins like recycling endosome coat proteins to form complexes critical for its function. It maintains the proper localization and recycling of surface proteins which is essential for maintaining cellular function.

Pathways

Regulation of vesicular transport by RAB11B integrates into several cellular pathways particularly the endocytic recycling pathway. This pathway supports the recycling of endocytosed material back to the plasma membrane. RAB11B interacts with other proteins such as RAB11A which has overlapping roles in vesicular transport to fine-tune the trafficking of molecules and maintain homeostasis.

RAB11B has links to conditions such as cancer and neurodegenerative diseases. Its dysregulation can result in abnormalities in vesicular trafficking which disrupts cellular homeostasis. For example in cancer altered RAB11B activity changes receptor recycling influencing cellular proliferation. In neurodegenerative diseases it may interact with amyloid precursor protein (APP) and facilitate events leading to disease progression. Changes in RAB11B function can associate with altered cell surface protein dynamics impacting disease development.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. The small Rab GTPase RAB11B plays a role in endocytic recycling, regulating apical recycling of several transmembrane proteins including cystic fibrosis transmembrane conductance regulator/CFTR, epithelial sodium channel/ENaC, potassium voltage-gated channel, and voltage-dependent L-type calcium channel. May also regulate constitutive and regulated secretion, like insulin granule exocytosis. Required for melanosome transport and release from melanocytes. Also regulates V-ATPase intracellular transport in response to extracellular acidosis. Promotes Rabin8/RAB3IP preciliary vesicular trafficking to mother centriole by forming a ciliary targeting complex containing Rab11, ASAP1, Rabin8/RAB3IP, RAB11FIP3 and ARF4, thereby regulating ciliogenesis initiation (Probable). On the contrary, upon LPAR1 receptor signaling pathway activation, interaction with phosphorylated WDR44 prevents Rab11-RAB3IP-RAB11FIP3 complex formation and cilia growth (Probable).
See full target information RAB11B
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:315 PubMed39747150

2025

CDC40 suppression induces CDCA5 splicing defects and anti-proliferative effects in lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Die Hu,Brigitte L Thériault,Vida Talebian,Laurent Hoffer,Julie Owen,Justin Lim,Benjamin J Blencowe,Evelyne Lima-Fernandes,Punit Saraon,Richard Marcellus,Rima Al-Awar

Cellular and molecular life sciences : CMLS 79:615 PubMed36460928

2022

The endocytic recycling compartment serves as a viral factory for hepatitis E virus.

Applications

Unspecified application

Species

Unspecified reactive species

Cyrine Bentaleb,Kévin Hervouet,Claire Montpellier,Charline Camuzet,Martin Ferrié,Julien Burlaud-Gaillard,Stéphane Bressanelli,Karoline Metzger,Elisabeth Werkmeister,Maliki Ankavay,Nancy Leon Janampa,Julien Marlet,Julien Roux,Clarence Deffaud,Anne Goffard,Yves Rouillé,Jean Dubuisson,Philippe Roingeard,Cécile-Marie Aliouat-Denis,Laurence Cocquerel

International journal of molecular sciences 22: PubMed34768768

2021

α-Galactosidase a Deficiency in Fabry Disease Leads to Extensive Dysregulated Cellular Signaling Pathways in Human Podocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Ulrich Jehn,Samet Bayraktar,Solvey Pollmann,Veerle Van Marck,Thomas Weide,Hermann Pavenstädt,Eva Brand,Malte Lenders
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB228954

Anti-RAB11B antibody

Immunocytochemistry/ Immunofluorescence - Anti-RAB11B antibody (AB228954)

  • 0
  • 0
Primary Antibodies

AB176344

Anti-RAB11B antibody [EPR12719]

RabMAb|Recombinant

Immunoprecipitation - Anti-RAB11B antibody [EPR12719] (AB176344)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB137029

Anti-RAB7 antibody [EPR7589] - Late Endosome Marker

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-RAB7 antibody [EPR7589] - Late Endosome Marker (AB137029)

  • 5
  • 24
Primary Antibodies

AB128913

Anti-Rab11A antibody [EPR7587(B)]

RabMAb|Recombinant

Immunoprecipitation - Anti-Rab11A antibody [EPR7587(B)] (AB128913)

  • 5
  • 2
Primary Antibodies

AB218624

Anti-Rab5 antibody [EPR21801] - Early Endosome Marker

KO Validated|RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-Rab5 antibody [EPR21801] - Early Endosome Marker (AB218624)

  • 4
  • 2
Primary Antibodies

AB109009

Anti-Rab4 antibody [EPR3043] - Early Endosome Marker

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (AB109009)

  • 1
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com