Rabbit Recombinant Monoclonal RAB11B antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Immunoprecipitation - Anti-RAB11B antibody [EPR12725] - BSA and Azide free (AB249892), expandable thumbnail](https://content.abcam.com/products/images/rab11b-antibody-epr12725-bsa-and-azide-free-ab249892--immunoprecipitation-img139812.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-RAB11B antibody [EPR12725] - BSA and Azide free (AB249892)")
![Western blot - Anti-RAB11B antibody [EPR12725] - BSA and Azide free (AB249892), expandable thumbnail](https://content.abcam.com/products/images/rab11b-antibody-epr12725-bsa-and-azide-free-ab249892--western-blot-img116354.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RAB11B antibody [EPR12725] - BSA and Azide free (AB249892)")
![Flow Cytometry (Intracellular) - Anti-RAB11B antibody [EPR12725] - BSA and Azide free (AB249892), expandable thumbnail](https://content.abcam.com/products/images/rab11b-antibody-epr12725-bsa-and-azide-free-ab249892--flow-cytometry-intracellular-img51001.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-RAB11B antibody [EPR12725] - BSA and Azide free (AB249892)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Not recommended | Tested |
| Mouse | Predicted | Predicted | Not recommended | Not recommended | Predicted |
| Rat | Predicted | Predicted | Not recommended | Not recommended | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. The small Rab GTPase RAB11B plays a role in endocytic recycling, regulating apical recycling of several transmembrane proteins including cystic fibrosis transmembrane conductance regulator/CFTR, epithelial sodium channel/ENaC, potassium voltage-gated channel, and voltage-dependent L-type calcium channel. May also regulate constitutive and regulated secretion, like insulin granule exocytosis. Required for melanosome transport and release from melanocytes. Also regulates V-ATPase intracellular transport in response to extracellular acidosis. Promotes Rabin8/RAB3IP preciliary vesicular trafficking to mother centriole by forming a ciliary targeting complex containing Rab11, ASAP1, Rabin8/RAB3IP, RAB11FIP3 and ARF4, thereby regulating ciliogenesis initiation (Probable). On the contrary, upon LPAR1 receptor signaling pathway activation, interaction with phosphorylated WDR44 prevents Rab11-RAB3IP-RAB11FIP3 complex formation and cilia growth (Probable).
Alternative names
YPT3, RAB11B, Ras-related protein Rab-11B, GTP-binding protein YPT3
Recommended products
Rabbit Recombinant Monoclonal RAB11B antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR12725
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249892 is the carrier-free version of Anti-RAB11B antibody [EPR12725] ab175925.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RAB11B also known as Ras-related protein Rab-11B is a small GTPase enzyme that belongs to the RAB protein family. RAB11B has a molecular mass of approximately 24 kDa. This protein is expressed in several tissues particularly in brain and testes. It plays a critical role in the regulation of membrane trafficking. As a member of the RAB family RAB11B cycles between an inactive GDP-bound form and an active GTP-bound form which is necessary for its function in vesicular transport.
- Biological function summary
Small GTPases like RAB11B govern intracellular vesicle trafficking between different organelles. It resides mainly in recycling endosomes and is important for the recycling of receptors and other cell surface proteins. RAB11B often associates with other proteins like recycling endosome coat proteins to form complexes critical for its function. It maintains the proper localization and recycling of surface proteins which is essential for maintaining cellular function.
- Pathways
Regulation of vesicular transport by RAB11B integrates into several cellular pathways particularly the endocytic recycling pathway. This pathway supports the recycling of endocytosed material back to the plasma membrane. RAB11B interacts with other proteins such as RAB11A which has overlapping roles in vesicular transport to fine-tune the trafficking of molecules and maintain homeostasis.
- Associated diseases and disorders
RAB11B has links to conditions such as cancer and neurodegenerative diseases. Its dysregulation can result in abnormalities in vesicular trafficking which disrupts cellular homeostasis. For example in cancer altered RAB11B activity changes receptor recycling influencing cellular proliferation. In neurodegenerative diseases it may interact with amyloid precursor protein (APP) and facilitate events leading to disease progression. Changes in RAB11B function can associate with altered cell surface protein dynamics impacting disease development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunoprecipitation - Anti-RAB11B antibody [EPR12725] - BSA and Azide free (ab249892), expandable thumbnail](https://content.abcam.com/products/images/rab11b-antibody-epr12725-bsa-and-azide-free-ab249892--immunoprecipitation-img139812.jpg "Immunoprecipitation - Anti-RAB11B antibody [EPR12725] - BSA and Azide free (ab249892)")
Immunoprecipitation - Anti-RAB11B antibody [EPR12725] - BSA and Azide free (ab249892)
This data was developed using Anti-RAB11B antibody [EPR12725] ab175925, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from (1) fetal brain lysate or (2) 1X PBS (negative control) using Anti-RAB11B antibody [EPR12725] ab175925 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
All lanes: Immunoprecipitation - Anti-RAB11B antibody [EPR12725] (Anti-RAB11B antibody [EPR12725] ab175925)
Predicted band size: 24 kDa
![Western blot - Anti-RAB11B antibody [EPR12725] - BSA and Azide free (ab249892), expandable thumbnail](https://content.abcam.com/products/images/rab11b-antibody-epr12725-bsa-and-azide-free-ab249892--western-blot-img116354.jpg "Western blot - Anti-RAB11B antibody [EPR12725] - BSA and Azide free (ab249892)")
Western blot - Anti-RAB11B antibody [EPR12725] - BSA and Azide free (ab249892)
This data was developed using Anti-RAB11B antibody [EPR12725] ab175925, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-RAB11B antibody [EPR12725] (Anti-RAB11B antibody [EPR12725] ab175925) at 1/1000 dilution
Lane 1: Fetal brain tissue lysate at 10 µg
Lane 2: 293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg
Lane 3: U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate at 10 µg
Lane 4: Jurkat cell lysate at 10 µg
Predicted band size: 24 kDa
![Flow Cytometry (Intracellular) - Anti-RAB11B antibody [EPR12725] - BSA and Azide free (ab249892), expandable thumbnail](https://content.abcam.com/products/images/rab11b-antibody-epr12725-bsa-and-azide-free-ab249892--flow-cytometry-intracellular-img51001.jpg "Flow Cytometry (Intracellular) - Anti-RAB11B antibody [EPR12725] - BSA and Azide free (ab249892)")
Flow Cytometry (Intracellular) - Anti-RAB11B antibody [EPR12725] - BSA and Azide free (ab249892)
This data was developed using Anti-RAB11B antibody [EPR12725] ab175925, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of Jurkat cells labeling RAB11B with Anti-RAB11B antibody [EPR12725] ab175925 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
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