AB249892

Anti-RAB11B antibody [EPR12725] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal RAB11B antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

YPT3, RAB11B, Ras-related protein Rab-11B, GTP-binding protein YPT3

3 Images

Flow Cytometry (Intracellular) - Anti-RAB11B antibody [EPR12725] - BSA and Azide free (AB249892)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-RAB11B antibody [EPR12725] - BSA and Azide free (AB249892)

This data was developed using ab175925, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of Jurkat cells labeling RAB11B with ab175925 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

Supplier Data

Immunoprecipitation - Anti-RAB11B antibody [EPR12725] - BSA and Azide free (AB249892)

This data was developed using ab175925, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from (1) fetal brain lysate or (2) 1X PBS (negative control) using ab175925 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-RAB11B antibody [EPR12725] (<a href='/en-us/products/primary-antibodies/rab11b-antibody-epr12725-ab175925'>ab175925</a>)

Predicted band size: 24 kDa

false

Supplier Data

Western blot - Anti-RAB11B antibody [EPR12725] - BSA and Azide free (AB249892)

This data was developed using ab175925, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RAB11B antibody [EPR12725] (<a href='/en-us/products/primary-antibodies/rab11b-antibody-epr12725-ab175925'>ab175925</a>) at 1/1000 dilution

Lane 1:

Fetal brain tissue lysate at 10 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 3:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Predicted band size: 24 kDa

false

Unconjugated

Anti-RAB11B antibody [EPR12725]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12725

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab249892 is the carrier-free version of ab175925.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAB11B also known as Ras-related protein Rab-11B is a small GTPase enzyme that belongs to the RAB protein family. RAB11B has a molecular mass of approximately 24 kDa. This protein is expressed in several tissues particularly in brain and testes. It plays a critical role in the regulation of membrane trafficking. As a member of the RAB family RAB11B cycles between an inactive GDP-bound form and an active GTP-bound form which is necessary for its function in vesicular transport.

Biological function summary

Small GTPases like RAB11B govern intracellular vesicle trafficking between different organelles. It resides mainly in recycling endosomes and is important for the recycling of receptors and other cell surface proteins. RAB11B often associates with other proteins like recycling endosome coat proteins to form complexes critical for its function. It maintains the proper localization and recycling of surface proteins which is essential for maintaining cellular function.

Pathways

Regulation of vesicular transport by RAB11B integrates into several cellular pathways particularly the endocytic recycling pathway. This pathway supports the recycling of endocytosed material back to the plasma membrane. RAB11B interacts with other proteins such as RAB11A which has overlapping roles in vesicular transport to fine-tune the trafficking of molecules and maintain homeostasis.

RAB11B has links to conditions such as cancer and neurodegenerative diseases. Its dysregulation can result in abnormalities in vesicular trafficking which disrupts cellular homeostasis. For example in cancer altered RAB11B activity changes receptor recycling influencing cellular proliferation. In neurodegenerative diseases it may interact with amyloid precursor protein (APP) and facilitate events leading to disease progression. Changes in RAB11B function can associate with altered cell surface protein dynamics impacting disease development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (PubMed : 14627637, PubMed : 19029296, PubMed : 19244346, PubMed : 20717956, PubMed : 21248079, PubMed : 22129970, PubMed : 26032412). RAB11B plays a role in endocytic recycling, regulating apical recycling of several transmembrane proteins including cystic fibrosis transmembrane conductance regulator/CFTR, epithelial sodium channel/ENaC, potassium voltage-gated channel, and voltage-dependent L-type calcium channel. May also regulate constitutive and regulated secretion, like insulin granule exocytosis. Required for melanosome transport and release from melanocytes. Also regulates V-ATPase intracellular transport in response to extracellular acidosis (PubMed : 14627637, PubMed : 19029296, PubMed : 19244346, PubMed : 20717956, PubMed : 21248079, PubMed : 22129970). Promotes Rabin8/RAB3IP preciliary vesicular trafficking to mother centriole by forming a ciliary targeting complex containing Rab11, ASAP1, Rabin8/RAB3IP, RAB11FIP3 and ARF4, thereby regulating ciliogenesis initiation (PubMed : 25673879). On the contrary, upon LPAR1 receptor signaling pathway activation, interaction with phosphorylated WDR44 prevents Rab11-RAB3IP-RAB11FIP3 complex formation and cilia growth (PubMed : 31204173). Also interacts with RABL3 to promote ciliary vesicle formation (PubMed : 36052645).

See full target information RAB11B

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 9:775642 PubMed35004677

2021

HIF-1α Induces HECTD2 Up-Regulation and Aggravates the Malignant Progression of Renal Cell Cancer Repressing miR-320a.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Lv,Taimin Shen,Juncheng Yao,Qi Yang,Ying Xiang,Zhiwei Ma

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RAB11B antibody [EPR12725] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-RAB11B antibody [EPR12725] - BSA and Azide free (AB249892)

Immunoprecipitation - Anti-RAB11B antibody [EPR12725] - BSA and Azide free (AB249892)

Western blot - Anti-RAB11B antibody [EPR12725] - BSA and Azide free (AB249892)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise