Rabbit Polyclonal RAB11FIP1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human RAB11FIP1 aa 400-550.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human RAB11FIP1 aa 400-550. The exact immunogen used to generate this antibody is proprietary information. Database link Q6WKZ4
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Target data
Function
A Rab11 effector protein involved in the endosomal recycling process. Also involved in controlling membrane trafficking along the phagocytic pathway and in phagocytosis.
Alternative names
RCP, RAB11FIP1, Rab11 family-interacting protein 1, Rab11-FIP1, Rab-coupling protein
Recommended products
Rabbit Polyclonal RAB11FIP1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human RAB11FIP1 aa 400-550.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human RAB11FIP1 aa 400-550. The exact immunogen used to generate this antibody is proprietary information. Database link Q6WKZ4
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RAB11FIP1 also known as Rab11 Family Interacting Protein 1 or pp75 is a protein that plays an important role in membrane trafficking. It acts as an effector molecule for Rab11 a small GTPase involved in the regulation of vesicle recycling. RAB11FIP1 has a mass of about 90 kDa and gets expressed in many tissues including brain liver and kidney. It contains regions critical for its interaction with Rab11 facilitating cargo sorting and transport within the endosomal recycling system.
- Biological function summary
RAB11FIP1 is involved in several cellular functions related to endosomal transport and recycling processes. It is part of the recycling endosome complex where it helps in the sorting and delivery of cargo to the plasma membrane. The protein contributes to processes like insulin-stimulated glucose transporter 4 translocation and transferrin receptor recycling indicating its role in important cellular functions. It also associates with various other proteins to ensure efficient endocytic and exocytic pathway operations.
- Pathways
RAB11FIP1 has significant roles in processes like the endocytic recycling pathway and the trans-Golgi network. Its interaction with Rab11 is part of the broader trafficking network regulating endocytic recycling critical for maintaining cell surface receptor levels. It is also linked with other proteins such as EHD1 known for their roles in endosomal transport highlighting its involvement in maintaining intracellular homeostasis and efficient transport of cellular material.
- Associated diseases and disorders
RAB11FIP1 contributes to conditions such as cancer and neurological disorders. Alterations in its function can disturb membrane trafficking pathways affecting processes like cell migration and proliferation linked to cancer development. Additionally its interactions with proteins such as E-cadherin important for cell adhesion further connect RAB11FIP1 to cancer progression. The protein is also implicated in neurological disorders due to its significant role in vesicle trafficking in neurons affecting synaptic function and potentially contributing to neurodegenerative diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Immunocytochemistry/ Immunofluorescence - Anti-RAB11FIP1 antibody (ab251765)
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for RAB11FIP1 (green) using ab251765 at 4 µg/ml in ICC/IF.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB11FIP1 antibody (ab251765)
Paraffin-embedded human small intestine tissue stained for RAB11FIP1 using ab251765 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB11FIP1 antibody (ab251765)
Paraffin-embedded human placenta tissue stained for RAB11FIP1 using ab251765 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB11FIP1 antibody (ab251765)
Paraffin-embedded human testis tissue stained for RAB11FIP1 using ab251765 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB11FIP1 antibody (ab251765)
Paraffin-embedded human skeletal muscle tissue stained for RAB11FIP1 using ab251765 at 1/50 dilution in immunohistochemical analysis.
Western blot - Anti-RAB11FIP1 antibody (ab251765)
All lanes: Western blot - Anti-RAB11FIP1 antibody (ab251765) at 0.4 µg/mL
Lane 1: A431 (Human epidermoid carcinoma cell line) cell lysate
Lane 2: U-251 MG (Human brain glioma cell line) cell lysate
Predicted band size: 137 kDa
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