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AB251765

Anti-RAB11FIP1 antibody

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(1 Publication)

Rabbit Polyclonal RAB11FIP1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RAB11FIP1 aa 400-550.

View Alternative Names

RCP, RAB11FIP1, Rab11 family-interacting protein 1, Rab11-FIP1, Rab-coupling protein

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB11FIP1 antibody (AB251765)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB11FIP1 antibody (AB251765)

Paraffin-embedded human placenta tissue stained for RAB11FIP1 using ab251765 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB11FIP1 antibody (AB251765)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB11FIP1 antibody (AB251765)

Paraffin-embedded human small intestine tissue stained for RAB11FIP1 using ab251765 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB11FIP1 antibody (AB251765)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB11FIP1 antibody (AB251765)

Paraffin-embedded human skeletal muscle tissue stained for RAB11FIP1 using ab251765 at 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-RAB11FIP1 antibody (AB251765)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RAB11FIP1 antibody (AB251765)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for RAB11FIP1 (green) using ab251765 at 4 µg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB11FIP1 antibody (AB251765)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB11FIP1 antibody (AB251765)

Paraffin-embedded human testis tissue stained for RAB11FIP1 using ab251765 at 1/50 dilution in immunohistochemical analysis.

Western blot - Anti-RAB11FIP1 antibody (AB251765)
  • WB

Unknown

Western blot - Anti-RAB11FIP1 antibody (AB251765)

All lanes:

Western blot - Anti-RAB11FIP1 antibody (ab251765) at 0.4 µg/mL

Lane 1:

A431 (Human epidermoid carcinoma cell line) cell lysate

Lane 2:

U-251 MG (Human brain glioma cell line) cell lysate

Predicted band size: 137 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human RAB11FIP1 aa 400-550. The exact immunogen used to generate this antibody is proprietary information.

Q6WKZ4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAB11FIP1 also known as Rab11 Family Interacting Protein 1 or pp75 is a protein that plays an important role in membrane trafficking. It acts as an effector molecule for Rab11 a small GTPase involved in the regulation of vesicle recycling. RAB11FIP1 has a mass of about 90 kDa and gets expressed in many tissues including brain liver and kidney. It contains regions critical for its interaction with Rab11 facilitating cargo sorting and transport within the endosomal recycling system.
Biological function summary

RAB11FIP1 is involved in several cellular functions related to endosomal transport and recycling processes. It is part of the recycling endosome complex where it helps in the sorting and delivery of cargo to the plasma membrane. The protein contributes to processes like insulin-stimulated glucose transporter 4 translocation and transferrin receptor recycling indicating its role in important cellular functions. It also associates with various other proteins to ensure efficient endocytic and exocytic pathway operations.

Pathways

RAB11FIP1 has significant roles in processes like the endocytic recycling pathway and the trans-Golgi network. Its interaction with Rab11 is part of the broader trafficking network regulating endocytic recycling critical for maintaining cell surface receptor levels. It is also linked with other proteins such as EHD1 known for their roles in endosomal transport highlighting its involvement in maintaining intracellular homeostasis and efficient transport of cellular material.

RAB11FIP1 contributes to conditions such as cancer and neurological disorders. Alterations in its function can disturb membrane trafficking pathways affecting processes like cell migration and proliferation linked to cancer development. Additionally its interactions with proteins such as E-cadherin important for cell adhesion further connect RAB11FIP1 to cancer progression. The protein is also implicated in neurological disorders due to its significant role in vesicle trafficking in neurons affecting synaptic function and potentially contributing to neurodegenerative diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

A Rab11 effector protein involved in the endosomal recycling process. Also involved in controlling membrane trafficking along the phagocytic pathway and in phagocytosis.
See full target information RAB11FIP1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of international medical research 52:3000605241282384 PubMed39628429

2024

RAB32 promotes glioma cell progression by activating the JAK/STAT3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Sinan Zhang,Xudong Jiang,Qing Wei,Liji Huang,Zhuoyan Huang,Lina Zhang
View all publications
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