JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB122666

Anti-RAB12 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal RAB12 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RAB12 aa 100-200.

View Alternative Names

Ras-related protein Rab-12, RAB12

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB12 antibody (AB122666)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB12 antibody (AB122666)

ab122666 at 1/50 dilution staining RAB12 in paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry.

Immunocytochemistry/ Immunofluorescence - Anti-RAB12 antibody (AB122666)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RAB12 antibody (AB122666)

Immunofluorescent staining of Human cell line U-2 OS shows positivity in vesicles. Recommended concentration of ab122666 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human RAB12 aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

Q6IQ22

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAB12 also known as Ras-related protein Rab-12 belongs to the Rab family of small GTPases and has a mass of approximately 25 kDa. This protein plays a role in membrane trafficking and vesicular transport. RAB12 localizes to the Golgi apparatus and other cellular membrane compartments. It expresses in various tissues with significant levels in the brain liver and skeletal muscle. This expression pattern suggests a broad role in cellular function.
Biological function summary

RAB12 regulates receptor degradation and cargo transport through endosomes and lysosomes. It participates in complex cellular processes involving endocytic recycling and autophagy. RAB12 interaction with other proteins such as RILP (Rab-interacting lysosomal protein) assists in regulating lysosome positioning. The protein modulates cellular dynamics which affects the degradation and recycling of receptor molecules.

Pathways

RAB12 plays an important part in the autophagy and endocytic recycling pathways. It cooperates with other Rab family members like RAB7 which is critical for late endosome to lysosome trafficking. In these pathways RAB12 functions as a molecular switch coordinating trafficking processes that impact cellular homeostasis. Its role in autophagy involves coordination of lysosomal degradation and its modulation influences the cellular response to nutrient availability.

RAB12 involvement connects to neurodegenerative disorders and cancer. It contributes to cellular processes that when dysfunctional lead to conditions like Parkinson's disease. Abnormal RAB12 activity can affect autophagy leading to the accumulation of protein aggregates in neurodegenerative diseases. Moreover RAB12 can interact with proteins like TBC1D10C (RABGAP-10) which influences cancer progression through its regulation of cellular growth and survival pathways.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab may play a role in protein transport from recycling endosomes to lysosomes regulating, for instance, the degradation of the transferrin receptor. Involved in autophagy (By similarity).
See full target information RAB12
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Virologica Sinica 39:501-512 PubMed38789039

2024

Calmodulin-like 5 promotes PEDV replication by regulating late-endosome synthesis and innate immune response.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Jun Tian,Xiu-Zhong Zhang,Jing Wang,Jian-Feng Liu,Fu-Huang Li,Xiao-Jia Wang
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB256487

Anti-RAB12 (phospho S106) antibody [MJF-R25-9]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (AB256487)

  • 0
  • 0
Primary Antibodies

AB256765

Anti-RAB12 (phospho S106) antibody [MJF-R25-9] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] - BSA and Azide free (AB256765)

  • 5
  • 1

Complementary Products

Primary Antibodies

AB205029

Anti-RABIF antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RABIF antibody (AB205029)

  • 0
  • 0
Primary Antibodies

AB133449

Anti-LRRK2 (phospho S910) antibody [UDD1 15(3)]

RabMAb|Recombinant

Western blot - Anti-LRRK2 (phospho S910) antibody [UDD1 15(3)] (AB133449)

  • 0
  • 0
Primary Antibodies

AB237703

Anti-RAB10 antibody [MJF-R23]

20ul selling size|KO Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-RAB10 antibody [MJF-R23] (AB237703)

  • 0
  • 0
Primary Antibodies

AB241062

Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1]

RabMAb|Recombinant

Western blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] (AB241062)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com