Rabbit Recombinant Monoclonal RAB12 antibody. Carrier free. Suitable for WB and reacts with Human, Rat samples.
Images
![Western blot - Anti-RAB12 antibody [EPR28814-81] - BSA and Azide free (AB316771), expandable thumbnail](https://content.abcam.com/products/images/rab12-antibody-epr28814-81-bsa-and-azide-free-ab316771--western-blot-img427811.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RAB12 antibody [EPR28814-81] - BSA and Azide free (AB316771)")
![Western blot - Anti-RAB12 antibody [EPR28814-81] - BSA and Azide free (AB316771), expandable thumbnail](https://content.abcam.com/products/images/rab12-antibody-epr28814-81-bsa-and-azide-free-ab316771--western-blot-img427810.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RAB12 antibody [EPR28814-81] - BSA and Azide free (AB316771)")
![Western blot - Anti-RAB12 antibody [EPR28814-81] - BSA and Azide free (AB316771), expandable thumbnail](https://content.abcam.com/products/images/rab12-antibody-epr28814-81-bsa-and-azide-free-ab316771--western-blot-img427809.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RAB12 antibody [EPR28814-81] - BSA and Azide free (AB316771)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IP | ICC/IF | Flow Cyt (Intra) | IHC-Fr | IHC-P | |
|---|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab may play a role in protein transport from recycling endosomes to lysosomes regulating, for instance, the degradation of the transferrin receptor. Involved in autophagy (By similarity).
Alternative names
Ras-related protein Rab-12, RAB12
Recommended products
Rabbit Recombinant Monoclonal RAB12 antibody. Carrier free. Suitable for WB and reacts with Human, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR28814-81
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab316771 is the carrirer-free version of Anti-RAB12 antibody [EPR28814-81] ab316770.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RAB12 also known as Ras-related protein Rab-12 belongs to the Rab family of small GTPases and has a mass of approximately 25 kDa. This protein plays a role in membrane trafficking and vesicular transport. RAB12 localizes to the Golgi apparatus and other cellular membrane compartments. It expresses in various tissues with significant levels in the brain liver and skeletal muscle. This expression pattern suggests a broad role in cellular function.
- Biological function summary
RAB12 regulates receptor degradation and cargo transport through endosomes and lysosomes. It participates in complex cellular processes involving endocytic recycling and autophagy. RAB12 interaction with other proteins such as RILP (Rab-interacting lysosomal protein) assists in regulating lysosome positioning. The protein modulates cellular dynamics which affects the degradation and recycling of receptor molecules.
- Pathways
RAB12 plays an important part in the autophagy and endocytic recycling pathways. It cooperates with other Rab family members like RAB7 which is critical for late endosome to lysosome trafficking. In these pathways RAB12 functions as a molecular switch coordinating trafficking processes that impact cellular homeostasis. Its role in autophagy involves coordination of lysosomal degradation and its modulation influences the cellular response to nutrient availability.
- Associated diseases and disorders
RAB12 involvement connects to neurodegenerative disorders and cancer. It contributes to cellular processes that when dysfunctional lead to conditions like Parkinson's disease. Abnormal RAB12 activity can affect autophagy leading to the accumulation of protein aggregates in neurodegenerative diseases. Moreover RAB12 can interact with proteins like TBC1D10C (RABGAP-10) which influences cancer progression through its regulation of cellular growth and survival pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-RAB12 antibody [EPR28814-81] - BSA and Azide free (ab316771), expandable thumbnail](https://content.abcam.com/products/images/rab12-antibody-epr28814-81-bsa-and-azide-free-ab316771--western-blot-img427811.jpg "Western blot - Anti-RAB12 antibody [EPR28814-81] - BSA and Azide free (ab316771)")
Western blot - Anti-RAB12 antibody [EPR28814-81] - BSA and Azide free (ab316771)
This data was developed using Anti-RAB12 antibody [EPR28814-81] ab316770, the same antibody clone in a different buffer formulation.
Low expression: liver (PMID: 8575079).
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-RAB12 antibody [EPR28814-81] (Anti-RAB12 antibody [EPR28814-81] ab316770) at 1/1000 dilution
Lane 1: Rat testis tissue lysate at 40 µg with 5% NFDM/TBST
Lane 2: Rat liver tissue lysate at 40 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 27 kDa, 36 kDa
Exposure time: 59s
![Western blot - Anti-RAB12 antibody [EPR28814-81] - BSA and Azide free (ab316771), expandable thumbnail](https://content.abcam.com/products/images/rab12-antibody-epr28814-81-bsa-and-azide-free-ab316771--western-blot-img427810.jpg "Western blot - Anti-RAB12 antibody [EPR28814-81] - BSA and Azide free (ab316771)")
Western blot - Anti-RAB12 antibody [EPR28814-81] - BSA and Azide free (ab316771)
This data was developed using Anti-RAB12 antibody [EPR28814-81] ab316770, the same antibody clone in a different buffer formulation.
Low expression: liver (PMID: 8575079).
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-RAB12 antibody [EPR28814-81] (Anti-RAB12 antibody [EPR28814-81] ab316770) at 1/1000 dilution
Lane 1: Human adrenal gland tissue lysate at 40 µg with 5% NFDM/TBST
Lane 2: Human testis tissue lysate at 40 µg with 5% NFDM/TBST
Lane 3: Human lung tissue lysate at 40 µg with 5% NFDM/TBST
Lane 4: Human liver tissue lysate at 40 µg with 5% NFDM/TBST
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 27 kDa, 36 kDa
Exposure time: 180s
![Western blot - Anti-RAB12 antibody [EPR28814-81] - BSA and Azide free (ab316771), expandable thumbnail](https://content.abcam.com/products/images/rab12-antibody-epr28814-81-bsa-and-azide-free-ab316771--western-blot-img427809.jpg "Western blot - Anti-RAB12 antibody [EPR28814-81] - BSA and Azide free (ab316771)")
Western blot - Anti-RAB12 antibody [EPR28814-81] - BSA and Azide free (ab316771)
This data was developed using Anti-RAB12 antibody [EPR28814-81] ab316770, the same antibody clone in a different buffer formulation.
Low expression: HaCat (PMID: 33718142).
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-RAB12 antibody [EPR28814-81] (Anti-RAB12 antibody [EPR28814-81] ab316770) at 1/1000 dilution
Lane 1: SiHa (human cervical epithelial cell) whole cell lysate at 40 µg with 5% NFDM/TBST
Lane 2: HaCaT (human skin keratinocyte) whole cell lysate at 40 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 27 kDa, 124 kDa
Exposure time: 59s
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