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AB326714

Anti-RAB12 antibody [MJF-D28814-65R] - BSA and Azide free

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Rabbit Recombinant Monoclonal RAB12 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse samples.

View Alternative Names

Ras-related protein Rab-12, RAB12

3 Images
Western blot - Anti-RAB12 antibody [MJF-D28814-65R] - BSA and Azide free (AB326714)
  • WB

Lab

Western blot - Anti-RAB12 antibody [MJF-D28814-65R] - BSA and Azide free (AB326714)

This data was developed using ab325799, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The data was kindly provided by a collaborator.

The identity of the bands higher than 45kda are unknown.

In Western blot, Anti-alpha Tubulin antibody - Loading Control (37KDa).

All lanes:

Western blot - Anti-RAB12 antibody [MJF-D28814-65R] (<a href='/en-us/products/primary-antibodies/rab12-antibody-mjf-d28814-65r-ab325799'>ab325799</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 (human lung carcinoma epithelial cell) whole cell lysate at 10 µg

Lane 2:

RAB12 knockout A549 whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 27 kDa,37 kDa

false

Exposure time: 180s

Western blot - Anti-RAB12 antibody [MJF-D28814-65R] - BSA and Azide free (AB326714)
  • WB

Lab

Western blot - Anti-RAB12 antibody [MJF-D28814-65R] - BSA and Azide free (AB326714)

This data was developed using ab325799, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : liver (PMID : 8575079, PMID : 1313420).

Samples are non-boiled as boiling may cause protein aggregation.

The identity of the bands higher than 45kda are unknown.

In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) 1 : 100000 (15Kda).

All lanes:

Western blot - Anti-RAB12 antibody [MJF-D28814-65R] (<a href='/en-us/products/primary-antibodies/rab12-antibody-mjf-d28814-65r-ab325799'>ab325799</a>) at 1/1000 dilution

Lane 1:

Mouse lung tissue lysate at 50 µg

Lane 2:

Mouse liver tissue lysate at 50 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 27 kDa,15 kDa

false

Exposure time: 3s

Western blot - Anti-RAB12 antibody [MJF-D28814-65R] - BSA and Azide free (AB326714)
  • WB

Lab

Western blot - Anti-RAB12 antibody [MJF-D28814-65R] - BSA and Azide free (AB326714)

This data was developed using ab325799, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The data was kindly provided by a collaborator.

The identity of the bands higher than 45kda are unknown.

In Western blot, Anti-alpha Tubulin antibody - Loading Control (37KDa).

All lanes:

Western blot - Anti-RAB12 antibody [MJF-D28814-65R] (<a href='/en-us/products/primary-antibodies/rab12-antibody-mjf-d28814-65r-ab325799'>ab325799</a>) at 1/1000 dilution

Lane 1:

Wild-type MEF (mouse embryo fibroblast) whole cell lysate at 10 µg

Lane 2:

RAB12 knockout MEF whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 27 kDa,37 kDa

false

Exposure time: 180s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

MJF-D28814-65R

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab326714 is the carrier-free version of ab325799

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Collaborations
This antibody was developed with support from The Michael J. Fox Foundation.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab may play a role in protein transport from recycling endosomes to lysosomes regulating, for instance, the degradation of the transferrin receptor. Involved in autophagy (By similarity).
See full target information RAB12
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com