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AB327396

Anti-RAB12 (phospho S106) antibody [MJF-D29764-507] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal RAB12 phospho S106 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

Ras-related protein Rab-12, RAB12

1 Images
Sandwich ELISA - Anti-RAB12 (phospho S106) antibody [MJF-D29764-507] - BSA and Azide free (Detector) (AB327396)
  • sELISA

Lab

Sandwich ELISA - Anti-RAB12 (phospho S106) antibody [MJF-D29764-507] - BSA and Azide free (Detector) (AB327396)

293T + Human LRRK2 (G2019S Mutant) + Human RAB12 Wildtype + Cal A : 293T cells transfected with a human LRRK2 (G2019S mutant) expression vector containing a his-tag and a human wildtype RAB12 expression vector containing a his-tag were treated with 100 nM Calyculin A for 30 minutes, whole cell lysate. Serial dilutions of lysates were analyzed. 293T + Empty Vector + Cal A : 293T cells transfected with an empty vector containing a his-tag were treated with 100 nM Calyculin A for 30 minutes, whole cell lysate. Serial dilutions of lysates were analyzed.

293T + Human LRRK2 (G2019S Mutant) + Cal A : 293T cells transfected with a human LRRK2 (G2019S mutant) expression vector containing a his-tag were treated with 100 nM Calyculin A for 30 minutes, whole cell lysate. Serial dilutions of lysates were analyzed.

293T + Human LRRK2 (G2019S Mutant) + Human RAB12 (S106A Mutant) + Cal A : 293T cells transfected with a human LRRK2 (G2019S mutant) expression vector containing a his-tag and a human RAB12 (S106A mutant) expression vector containing a his-tag were treated with 100 nM Calyculin A for 30 minutes, whole cell lysate. Serial dilutions of lysates were analyzed.

  • Unconjugated

    Anti-RAB12 (phospho S106) antibody [MJF-D29764-507]

  • Carrier free

    Anti-RAB12 (phospho S106) antibody [MJF-D29764-507] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

MJF-D29764-507

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

ab327396 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human RAB12 (phospho S106). The recommended pair for sandwich ELISA is:

Capture: ab327395, Human RAB12 (phospho S106) Capture Antibody (unconjugated)

Detector: ab327396, Human RAB12 (phospho S106) Detector Antibody (unconjugated) .

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Collaborations
This antibody was developed with support from The Michael J. Fox Foundation.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (By similarity). RAB12 may play a role in protein transport from recycling endosomes to lysosomes regulating, for instance, the degradation of the transferrin receptor. Involved in autophagy (By similarity).
See full target information RAB12 pS106
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com