AB256487

Anti-RAB12 (phospho S106) antibody [MJF-R25-9]

RabMAb
Recombinant
20ul selling size
What is this?

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(19 Publications)

Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487) is a rabbit monoclonal antibody detecting RAB12 in Western Blot, Dot Blot. Suitable for Mouse,.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

Ras-related protein Rab-12, RAB12

3 Images

Lab

Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (AB256487)

This antibody was used in the Mir, Tonelli et al. 2018 paper (PMID : 29743203).

MLi-2 is a potent, selective LARRK2 kinase inhibitor (PMID : 26407721).

The lysates were kindly provided by Professor Dario Alessi from University of Dundee.

The antibody also reacts with unidentifiable proteins greater than 60 kDa.

Blocking/Dilution buffer : AdvanBlock.

All lanes:

Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487) at 1/1000 dilution

Lane 1:

Mouse embryonic fibroblasts from LRRK2[R1441C] knock in mice without MLi-2 treatment, whole cell lysate at 20 µg

Lane 2:

Mouse embryonic fibroblasts from LRRK2[R1441C] knock in mice treated with 100nM MLi-2 for 90 min, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 27 kDa

false

Exposure time: 48s

Lab

Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (AB256487)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487) at 1/1000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) transfected with an empty vector, containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T transfected with RAB12 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 27 kDa

Observed band size: 30 kDa

false

Exposure time: 120s

Lab

Dot Blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (AB256487)

Dot Blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487 at a 1/1000 dilution).

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary at 1/100,000 dilution.

Lane 1 : RAB12 (phospho S106) peptide.

Lane 2 : RAB12 non-phospho peptide.

Exposure time : 10 seconds.

Blocking/Dilution buffer : 5% NFDM/TBST.

Carrier free

Anti-RAB12 (phospho S106) antibody [MJF-R25-9] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

MJF-R25-9

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

What is this antibody validated in?
Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Dot Blot in Mouse, samples.

What is the molecular weight of RAB12?
Anti-RAB12 (phospho S106) [MJF-R25-9] (ab256487) specifically detects a band for RAB12 (UniProt: Q6IQ22) at a molecular weight of 27kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [MJF-R25-9] also available for your convenience: ab256487, Carrier free - ab256765

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Collaborations
This antibody was developed with support from The Michael J. Fox Foundation.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAB12 also known as Ras-related protein Rab-12 belongs to the Rab family of small GTPases and has a mass of approximately 25 kDa. This protein plays a role in membrane trafficking and vesicular transport. RAB12 localizes to the Golgi apparatus and other cellular membrane compartments. It expresses in various tissues with significant levels in the brain liver and skeletal muscle. This expression pattern suggests a broad role in cellular function.

Biological function summary

RAB12 regulates receptor degradation and cargo transport through endosomes and lysosomes. It participates in complex cellular processes involving endocytic recycling and autophagy. RAB12 interaction with other proteins such as RILP (Rab-interacting lysosomal protein) assists in regulating lysosome positioning. The protein modulates cellular dynamics which affects the degradation and recycling of receptor molecules.

Pathways

RAB12 plays an important part in the autophagy and endocytic recycling pathways. It cooperates with other Rab family members like RAB7 which is critical for late endosome to lysosome trafficking. In these pathways RAB12 functions as a molecular switch coordinating trafficking processes that impact cellular homeostasis. Its role in autophagy involves coordination of lysosomal degradation and its modulation influences the cellular response to nutrient availability.

RAB12 involvement connects to neurodegenerative disorders and cancer. It contributes to cellular processes that when dysfunctional lead to conditions like Parkinson's disease. Abnormal RAB12 activity can affect autophagy leading to the accumulation of protein aggregates in neurodegenerative diseases. Moreover RAB12 can interact with proteins like TBC1D10C (RABGAP-10) which influences cancer progression through its regulation of cellular growth and survival pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (By similarity). RAB12 may play a role in protein transport from recycling endosomes to lysosomes regulating, for instance, the degradation of the transferrin receptor. Involved in autophagy (By similarity).

See full target information RAB12 pS106

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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 44:116031 PubMed40690364

2025

PPM1M, an LRRK2-counteracting, phosphoRab12-preferring phosphatase with a potential link to Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Claire Y Chiang,Neringa Pratuseviciute,Yu-En Lin,Ayan Adhikari,Wondwossen M Yeshaw,Chloe Flitton,Pemba L Sherpa,Francesca Tonelli,Irena Rektorova,Timothy Lynch,Joanna Siuda,Monika Rudzińska-Bar,Oleksandr Pulyk,Peter Bauer,Christian Beetz,Dennis W Dickson,Owen A Ross,Zbigniew K Wszolek,Zih-Hua Fang,Christine Klein,Alexander Zimprich,Dario R Alessi,Esther M Sammler,Suzanne R Pfeffer

NPJ Parkinson's disease 11:106 PubMed40319043

2025

Emergent glutamate & dopamine dysfunction in VPS35 knock-in mice and rapid reversal by LRRK2 inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

A Kamesh,C A Kadgien,N Kuhlmann,S Coady,A Pietrantonio,Y Cousineau,A Khayachi,A Jurado Santos,E P Hurley,J C Barron,M P Parsons,A J Milnerwood

BMC neuroscience 26:19 PubMed40038582

2025

Short-term lipopolysaccharide treatment leads to astrocyte activation in LRRK2 G2019S knock-in mice without loss of dopaminergic neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Hoang Kieu Chi Ngo,Akriti Srivastava,Hoang Le,Samuel J Ayer,Grace F Crotty,Michael A Schwarzschild,Rachit Bakshi

iScience 27:110980 PubMed39635134

2024

Rebalance of mitophagy by inhibiting LRRK2 improves colon alterations in an MPTP model.

Applications

Unspecified application

Species

Unspecified reactive species

Alessia Filippone,Deborah Mannino,Laura Cucinotta,Fabrizio Calapai,Lelio Crupi,Irene Paterniti,Emanuela Esposito

Science immunology 9:eadi7907 PubMed39514635

2024

Macrophage LRRK2 hyperactivity impairs autophagy and induces Paneth cell dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Shengxiang Sun,Miki Hodel,Xiang Wang,Javier De Vicente,Talin Haritunians,Anketse Debebe,Chen-Ting Hung,Changqing Ma,Atika Malique,Hoang N Nguyen,Maayan Agam,Michael T Maloney,Marisa S Goo,Jillian H Kluss,Richa Mishra,Jennifer Frein,Amanda Foster,Samuel Ballentine,Uday Pandey,Justin Kern,Shaohong Yang,Emebet Mengesha,Iyshwarya Balasubramanian,Annie Arguello,Anthony A Estrada,Nan Gao,Inga Peter,Dermot P B McGovern,Anastasia G Henry,Thaddeus S Stappenbeck,Ta-Chiang Liu

NPJ Parkinson's disease 10:133 PubMed39030200

2024

Adult-onset deletion of ATP13A2 in mice induces progressive nigrostriatal pathway dopaminergic degeneration and lysosomal abnormalities.

Applications

Unspecified application

Species

Unspecified reactive species

Madalynn L Erb,Kayla Sipple,Nathan Levine,Xi Chen,Darren J Moore

The Biochemical journal 481:265-278 PubMed38299383

2024

The Parkinson's disease related mutant VPS35 (D620N) amplifies the LRRK2 response to endolysosomal stress.

Applications

Unspecified application

Species

Unspecified reactive species

Katy R McCarron,Hannah Elcocks,Heather Mortiboys,Sylvie Urbé,Michael J Clague

NPJ Parkinson's disease 10:12 PubMed38191886

2024

A potential patient stratification biomarker for Parkinson´s disease based on LRRK2 kinase-mediated centrosomal alterations in peripheral blood-derived cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yahaira Naaldijk,Belén Fernández,Rachel Fasiczka,Elena Fdez,Coline Leghay,Ioana Croitoru,John B Kwok,Yanisse Boulesnane,Amelie Vizeneux,Eugenie Mutez,Camille Calvez,Alain Destée,Jean-Marc Taymans,Ana Vinagre Aragon,Alberto Bergareche Yarza,Shalini Padmanabhan,Mario Delgado,Roy N Alcalay,Zac Chatterton,Nicolas Dzamko,Glenda Halliday,Javier Ruiz-Martínez,Marie-Christine Chartier-Harlin,Sabine Hilfiker

eLife 12: PubMed37874635

2023

Genome-wide screen reveals Rab12 GTPase as a critical activator of Parkinson's disease-linked LRRK2 kinase.

Applications

Unspecified application

Species

Unspecified reactive species

Herschel S Dhekne,Francesca Tonelli,Wondwossen M Yeshaw,Claire Y Chiang,Charles Limouse,Ebsy Jaimon,Elena Purlyte,Dario R Alessi,Suzanne R Pfeffer

The Journal of biological chemistry 299:105192 PubMed37625589

2023

Endogenous Rab38 regulates LRRK2's membrane recruitment and substrate Rab phosphorylation in melanocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra Unapanta,Farbod Shavarebi,Jacob Porath,Yiyi Shen,Carson Balen,Albert Nguyen,Josh Tseng,Weng Si Leong,Michelle Liu,Pawel Lis,Santiago M Di Pietro,Annie Hiniker

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RAB12 (phospho S106) antibody [MJF-R25-9]

Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (AB256487)

Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (AB256487)

Dot Blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (AB256487)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (19)

Product promise