Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487) is a rabbit monoclonal antibody that is used to detect RAB12 in Western Blot, Dot Blot. Suitable for Mouse, samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
Images
![Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (AB256487), expandable thumbnail](https://content.abcam.com/products/images/rab12-phospho-s106-antibody-mjf-r25-9-ab256487--western-blot-img164639.png/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (AB256487)")
![Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (AB256487), expandable thumbnail](https://content.abcam.com/products/images/rab12-phospho-s106-antibody-mjf-r25-9-ab256487--western-blot-img77705.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (AB256487)")
![Dot Blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (AB256487), expandable thumbnail](https://content.abcam.com/products/images/rab12-phospho-s106-antibody-mjf-r25-9-ab256487--dot-blot-img21925.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (AB256487)")
Publications
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- The Biochemical journal 477:4397-44232020Endogenous Rab29 does not impact basal or stimulated LRRK2 pathway activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexia F Kalogeropulou,Jordana B Freemantle,Pawel Lis,Edmundo G Vides,Nicole K Polinski,Dario R AlessiPubMed 33135724
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Dot | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Predicted | Predicted | Not recommended | Not recommended |
| Mouse | Not recommended | Expected | Tested | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Synthetic peptide | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Synthetic peptide, Rat | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes The antibody also reacts with unidentifiable proteins greater than 60 kDa. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Synthetic peptide | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Synthetic peptide, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab may play a role in protein transport from recycling endosomes to lysosomes regulating, for instance, the degradation of the transferrin receptor. Involved in autophagy (By similarity).
Alternative names
Ras-related protein Rab-12, RAB12
Recommended products
Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487) is a rabbit monoclonal antibody that is used to detect RAB12 in Western Blot, Dot Blot. Suitable for Mouse, samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- MJF-R25-9
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Dot Blot, in Mouse, samples.
What is the molecular weight of RAB12?
Anti-RAB12 (phospho S106) [MJF-R25-9] (ab256487) specifically detects a band for RAB12 (UniProt: Q6IQ22) at a molecular weight of 27kDa.
Recommended positive controls
Dot Blot: RAB12 (phospho S106) peptide.WB: Mouse embryonic fibroblasts from LRRK2[R1441C] knock in mice without MLi-2 treatment whole cell lysate, 293T transfected with RAB12 expression vector containing a myc-His-tag®, whole cell lysate.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.Other related products
We have a range of other formats of antibody clone [MJF-R25-9] also available for your convenience:
ab256487, Carrier free - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] - BSA and Azide free ab256765
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Collaborations
This antibody was developed with support from The Michael J. Fox Foundation.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RAB12 also known as Ras-related protein Rab-12 belongs to the Rab family of small GTPases and has a mass of approximately 25 kDa. This protein plays a role in membrane trafficking and vesicular transport. RAB12 localizes to the Golgi apparatus and other cellular membrane compartments. It expresses in various tissues with significant levels in the brain liver and skeletal muscle. This expression pattern suggests a broad role in cellular function.
- Biological function summary
RAB12 regulates receptor degradation and cargo transport through endosomes and lysosomes. It participates in complex cellular processes involving endocytic recycling and autophagy. RAB12 interaction with other proteins such as RILP (Rab-interacting lysosomal protein) assists in regulating lysosome positioning. The protein modulates cellular dynamics which affects the degradation and recycling of receptor molecules.
- Pathways
RAB12 plays an important part in the autophagy and endocytic recycling pathways. It cooperates with other Rab family members like RAB7 which is critical for late endosome to lysosome trafficking. In these pathways RAB12 functions as a molecular switch coordinating trafficking processes that impact cellular homeostasis. Its role in autophagy involves coordination of lysosomal degradation and its modulation influences the cellular response to nutrient availability.
- Associated diseases and disorders
RAB12 involvement connects to neurodegenerative disorders and cancer. It contributes to cellular processes that when dysfunctional lead to conditions like Parkinson's disease. Abnormal RAB12 activity can affect autophagy leading to the accumulation of protein aggregates in neurodegenerative diseases. Moreover RAB12 can interact with proteins like TBC1D10C (RABGAP-10) which influences cancer progression through its regulation of cellular growth and survival pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487), expandable thumbnail](https://content.abcam.com/products/images/rab12-phospho-s106-antibody-mjf-r25-9-ab256487--western-blot-img164639.png "Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487)")
Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Observed Mw 30kDa
All lanes: Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487) at 1/1000 dilution
Lane 1: 293T (Human embryonic kidney epithelial cell) transfected with an empty vector, containing a myc-His-tag®, whole cell lysate at 20 µg
Lane 2: 293T transfected with RAB12 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 27 kDa
Exposure time: 120s
![Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487), expandable thumbnail](https://content.abcam.com/products/images/rab12-phospho-s106-antibody-mjf-r25-9-ab256487--western-blot-img77705.jpg "Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487)")
Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487)
This antibody was used in the Mir, Tonelli et al. 2018 paper (PMID: 29743203).MLi-2 is a potent, selective LARRK2 kinase inhibitor (PMID: 26407721).
The lysates were kindly provided by Professor Dario Alessi from University of Dundee.
The antibody also reacts with unidentifiable proteins greater than 60 kDa.
Blocking/Dilution buffer: AdvanBlock.
All lanes: Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487) at 1/1000 dilution
Lane 1: Mouse embryonic fibroblasts from LRRK2[R1441C] knock in mice without MLi-2 treatment, whole cell lysate at 20 µg
Lane 2: Mouse embryonic fibroblasts from LRRK2[R1441C] knock in mice treated with 100nM MLi-2 for 90 min, whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 27 kDa
Exposure time: 48s
![Dot Blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487), expandable thumbnail](https://content.abcam.com/products/images/rab12-phospho-s106-antibody-mjf-r25-9-ab256487--dot-blot-img21925.jpg "Dot Blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487)")
Dot Blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487)
Dot Blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487 at a 1/1000 dilution).
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used as the secondary at 1/100,000 dilution.
Lane 1: RAB12 (phospho S106) peptide.
Lane 2: RAB12 non-phospho peptide.
Exposure time: 10 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
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