AB256765

Anti-RAB12 (phospho S106) antibody [MJF-R25-9] - BSA and Azide free

Name: Anti-RAB12 (phospho S106) antibody [MJF-R25-9] - BSA and Azide free (ab256765) | Abcam
Brand: Abcam Limited
SKU: AB256765
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(1 Publication)

Rabbit Recombinant Monoclonal RAB12 phospho S106 antibody. Carrier free. Suitable for Dot, WB and reacts with Synthetic peptide, Mouse, Human, Transfected cell lysate samples. Cited in 1 publication.

View Alternative Names

Ras-related protein Rab-12, RAB12

3 Images

Lab

Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] - BSA and Azide free (AB256765)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256487).

This antibody was used in the Mir, Tonelli et al. 2018 paper (PMID : 29743203).

MLi-2 is a potent, selective LARRK2 kinase inhibitor (PMID : 26407721).

The lysates were kindly provided by Professor Dario Alessi from University of Dundee.

The antibody also reacts with unidentifiable proteins greater than 60 kDa.

Blocking/Dilution buffer : AdvanBlock.

All lanes:

Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (<a href='/en-us/products/primary-antibodies/rab12-phospho-s106-antibody-mjf-r25-9-ab256487'>ab256487</a>) at 1/1000 dilution

Lane 1:

Mouse embryonic fibroblasts from LRRK2[R1441C] knock in mice without MLi-2 treatment, whole cell lysate at 20 µg

Lane 2:

Mouse embryonic fibroblasts from LRRK2[R1441C] knock in mice treated with 100nM MLi-2 for 90 min, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 27 kDa

false

Exposure time: 48s

Lab

Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] - BSA and Azide free (AB256765)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256487).

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (<a href='/en-us/products/primary-antibodies/rab12-phospho-s106-antibody-mjf-r25-9-ab256487'>ab256487</a>) at 1/1000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) transfected with an empty vector, containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T transfected with RAB12 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 27 kDa

Observed band size: 30 kDa

false

Exposure time: 120s

Lab

Dot Blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] - BSA and Azide free (AB256765)

Dot Blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487 at a 1/1000 dilution).

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary at 1/100,000 dilution.

Lane 1 : RAB12 (phospho S106) peptide.

Lane 2 : RAB12 non-phospho peptide.

Exposure time : 10 seconds.

Blocking/Dilution buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256487).

Unconjugated

Anti-RAB12 (phospho S106) antibody [MJF-R25-9]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

MJF-R25-9

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "The antibody also reacts with unidentifiable proteins greater than 60 kDa." }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Synthetic peptide": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab256765 is the carrier-free version of ab256487.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Collaborations
This antibody was developed with support from The Michael J. Fox Foundation.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAB12 also known as Ras-related protein Rab-12 belongs to the Rab family of small GTPases and has a mass of approximately 25 kDa. This protein plays a role in membrane trafficking and vesicular transport. RAB12 localizes to the Golgi apparatus and other cellular membrane compartments. It expresses in various tissues with significant levels in the brain liver and skeletal muscle. This expression pattern suggests a broad role in cellular function.

Biological function summary

RAB12 regulates receptor degradation and cargo transport through endosomes and lysosomes. It participates in complex cellular processes involving endocytic recycling and autophagy. RAB12 interaction with other proteins such as RILP (Rab-interacting lysosomal protein) assists in regulating lysosome positioning. The protein modulates cellular dynamics which affects the degradation and recycling of receptor molecules.

Pathways

RAB12 plays an important part in the autophagy and endocytic recycling pathways. It cooperates with other Rab family members like RAB7 which is critical for late endosome to lysosome trafficking. In these pathways RAB12 functions as a molecular switch coordinating trafficking processes that impact cellular homeostasis. Its role in autophagy involves coordination of lysosomal degradation and its modulation influences the cellular response to nutrient availability.

RAB12 involvement connects to neurodegenerative disorders and cancer. It contributes to cellular processes that when dysfunctional lead to conditions like Parkinson's disease. Abnormal RAB12 activity can affect autophagy leading to the accumulation of protein aggregates in neurodegenerative diseases. Moreover RAB12 can interact with proteins like TBC1D10C (RABGAP-10) which influences cancer progression through its regulation of cellular growth and survival pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab may play a role in protein transport from recycling endosomes to lysosomes regulating, for instance, the degradation of the transferrin receptor. Involved in autophagy (By similarity).

See full target information RAB12 phospho S106

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Aging cell 20:e13454 PubMed34510683

2021

microRNA-425 loss mediates amyloid plaque microenvironment heterogeneity and promotes neurodegenerative pathologies.

Applications

Unspecified application

Species

Unspecified reactive species

Yong-Bo Hu,Yong-Fang Zhang,Ru-Jing Ren,Eric B Dammer,Xin-Yi Xie,Shi-Wu Chen,Qiang Huang,Wan-Ying Huang,Rui Zhang,Hong-Zhuan Chen,Hao Wang,Gang Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RAB12 (phospho S106) antibody [MJF-R25-9] - BSA and Azide free

Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] - BSA and Azide free (AB256765)

Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] - BSA and Azide free (AB256765)

Dot Blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] - BSA and Azide free (AB256765)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise