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Rabbit Polyclonal RAB18 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Ras-related protein Rab-18.

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Images

Western blot - Anti-Rab18 antibody (AB119900), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab18 antibody (AB119900), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 1% BSA

Form

Lyophilized

Clonality

Polyclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human Ras-related protein Rab-18. Database link Q9NP72

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested
Rat
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/2000

Notes

-

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100 - 1/500

Notes

-

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes (PubMed:24891604, PubMed:30970241). Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (PubMed:24891604, PubMed:30970241). Required for the localization of ZFYVE1 to lipid droplets and for its function in mediating the formation of endoplasmic reticulum-lipid droplets (ER-LD) contacts (PubMed:30970241). Also required for maintaining endoplasmic reticulum structure (PubMed:24891604). Plays a role in apical endocytosis/recycling (By similarity). Plays a key role in eye and brain development and neurodegeneration (PubMed:21473985).

Alternative names

Recommended products

Rabbit Polyclonal RAB18 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Ras-related protein Rab-18.

Key facts

Isotype

IgG

Form

Lyophilized

Clonality

Polyclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Human Ras-related protein Rab-18. Database link Q9NP72
Purification technique

Affinity purification Immunogen

Reconstitution

Reconstitute in 200 µL of water

Concentration
Loading...
Purification notes

ab119900 is purified by a protein affinity column.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Rab18 also known as RAB18A or RAB18 is a member of the Rab GTPase family a group of proteins involved in intracellular vesicle trafficking. Rab18 is approximately 23 kDa in mass. Researchers find Rab18 expressed widely in various tissues including the brain liver and adipose tissue. As a small GTPase Rab18 switches between an inactive GDP-bound state and an active GTP-bound state regulating membrane trafficking and fusion processes.

Biological function summary

The functions of Rab18 extend beyond simple vesicular transport. Rab18 interacts with lipid droplets and the endoplasmic reticulum influencing lipid metabolism and storage. It is sometimes present in a complex with proteins like TBC1D20 which acts as a GAP (GTPase-activating protein) that further regulates Rab18’s activity. Through these interactions Rab18 influences lipid homeostasis and endoplasmic reticulum organization adding a layer of control to cellular lipid balance.

Pathways

Rab18 participates in the regulation of lipid droplet growth and maturation linking it to lipid metabolism pathways. Its activity plays a role in the tethering of lipid droplets to the endoplasmic reticulum relating it functionally to proteins such as Seipin. Additionally Rab18 is involved in intracellular transport pathways where it cooperates with other Rab proteins like Rab3 and Rab10 to facilitate membrane trafficking and organelle dynamics.

Associated diseases and disorders

Rab18 associations are evident in conditions like Warburg Micro syndrome and intellectual disability. Warburg Micro syndrome characterized by eye and brain abnormalities emerges from mutations affecting Rab18 or its interacting partners like RAB3GAP. Similarly Rab18’s involvement in lipid metabolism connects it with metabolic disorders where lipid storage and synthesis disruptions play a role. This links the functionality of Rab18 in cellular processes to broader impacts on human health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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2 product images

  • Western blot - Anti-Rab18 antibody (ab119900), expandable thumbnail

    Western blot - Anti-Rab18 antibody (ab119900)

    All lanes: Western blot - Anti-Rab18 antibody (ab119900) at 1/1000 dilution

    All lanes: Human fetal testis lysate

    Predicted band size: 23 kDa, 24 kDa

    Observed band size: 23 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab18 antibody (ab119900), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab18 antibody (ab119900)

    ab119900 at 1/100 dilution staining Rab18 in Formalin-fixed, Paraffin-embedded Human prostate carcinoma tissue by Immunohistochemistry.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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