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AB119900

Anti-Rab18 antibody

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(3 Publications)

Rabbit Polyclonal RAB18 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Ras-related protein Rab-18.

View Alternative Names

Ras-related protein Rab-18, RAB18

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab18 antibody (AB119900)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab18 antibody (AB119900)

ab119900 at 1/100 dilution staining Rab18 in Formalin-fixed, Paraffin-embedded Human prostate carcinoma tissue by Immunohistochemistry.

Western blot - Anti-Rab18 antibody (AB119900)
  • WB

Unknown

Western blot - Anti-Rab18 antibody (AB119900)

All lanes:

Western blot - Anti-Rab18 antibody (ab119900) at 1/1000 dilution

All lanes:

Human fetal testis lysate

Predicted band size: 23 kDa,24 kDa

Observed band size: 23 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human Ras-related protein Rab-18.

Q9NP72

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Lyophilized
Reconstitution
Reconstitute in 200 µL of water
Purification technique
Affinity purification Immunogen
Purification notes
ab119900 is purified by a protein affinity column.
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rab18 also known as RAB18A or RAB18 is a member of the Rab GTPase family a group of proteins involved in intracellular vesicle trafficking. Rab18 is approximately 23 kDa in mass. Researchers find Rab18 expressed widely in various tissues including the brain liver and adipose tissue. As a small GTPase Rab18 switches between an inactive GDP-bound state and an active GTP-bound state regulating membrane trafficking and fusion processes.
Biological function summary

The functions of Rab18 extend beyond simple vesicular transport. Rab18 interacts with lipid droplets and the endoplasmic reticulum influencing lipid metabolism and storage. It is sometimes present in a complex with proteins like TBC1D20 which acts as a GAP (GTPase-activating protein) that further regulates Rab18’s activity. Through these interactions Rab18 influences lipid homeostasis and endoplasmic reticulum organization adding a layer of control to cellular lipid balance.

Pathways

Rab18 participates in the regulation of lipid droplet growth and maturation linking it to lipid metabolism pathways. Its activity plays a role in the tethering of lipid droplets to the endoplasmic reticulum relating it functionally to proteins such as Seipin. Additionally Rab18 is involved in intracellular transport pathways where it cooperates with other Rab proteins like Rab3 and Rab10 to facilitate membrane trafficking and organelle dynamics.

Rab18 associations are evident in conditions like Warburg Micro syndrome and intellectual disability. Warburg Micro syndrome characterized by eye and brain abnormalities emerges from mutations affecting Rab18 or its interacting partners like RAB3GAP. Similarly Rab18’s involvement in lipid metabolism connects it with metabolic disorders where lipid storage and synthesis disruptions play a role. This links the functionality of Rab18 in cellular processes to broader impacts on human health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes (PubMed : 24891604, PubMed : 30970241). Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (PubMed : 24891604, PubMed : 30970241). RAB18 is required for the localization of ZFYVE1 to lipid droplets and for its function in mediating the formation of endoplasmic reticulum-lipid droplets (ER-LD) contacts (PubMed : 30970241). Also required for maintaining endoplasmic reticulum structure (PubMed : 24891604). Plays a role in apical endocytosis/recycling (By similarity). Plays a key role in eye and brain development and neurodegeneration (PubMed : 21473985).
See full target information Ras-related protein Rab-18
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 10:958566 PubMed36158190

2022

Perilipin-2 promotes lipid droplet-plasma membrane interactions that facilitate apocrine lipid secretion in secretory epithelial cells of the mouse mammary gland.

Applications

Unspecified application

Species

Unspecified reactive species

Jenifer Monks,David J Orlicky,Andrew E Libby,Monica Dzieciatkowska,Mark S Ladinsky,James L McManaman

Nature communications 12:5772 PubMed34599178

2021

Ring finger protein 213 assembles into a sensor for ISGylated proteins with antimicrobial activity.

Applications

Unspecified application

Species

Unspecified reactive species

Fabien Thery,Lia Martina,Caroline Asselman,Yifeng Zhang,Madeleine Vessely,Heidi Repo,Koen Sedeyn,George D Moschonas,Clara Bredow,Qi Wen Teo,Jingshu Zhang,Kevin Leandro,Denzel Eggermont,Delphine De Sutter,Katie Boucher,Tino Hochepied,Nele Festjens,Nico Callewaert,Xavier Saelens,Bart Dermaut,Klaus-Peter Knobeloch,Antje Beling,Sumana Sanyal,Lilliana Radoshevich,Sven Eyckerman,Francis Impens

Hepatology (Baltimore, Md.) 62:615-26 PubMed25482505

2014

Liver X receptors balance lipid stores in hepatic stellate cells through Rab18, a retinoid responsive lipid droplet protein.

Applications

ICC/IF

Species

Mouse

Fiona O'Mahony,Kevin Wroblewski,Sheila M O'Byrne,Hongfeng Jiang,Kara Clerkin,Jihane Benhammou,William S Blaner,Simon W Beaven
View all publications
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