AB182764

Anti-Rab18 antibody

Name: Anti-Rab18 antibody (ab182764) | Abcam
Brand: Abcam Limited
SKU: AB182764
Price: 535 USD
Availability: InStock

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Polyclonal RAB18 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human Ras-related protein Rab-18 aa 100-150.

View Alternative Names

Ras-related protein Rab-18, RAB18

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab18 antibody (AB182764)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab18 antibody (AB182764)

Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling Rab18 with ab182764 at 1/300 dilution, in the presence (right panel) or absence (left panel) of immunizing peptide.

Supplier Data

Western blot - Anti-Rab18 antibody (AB182764)

Gel : 10% SDS-PAGE

All lanes:

Western blot - Anti-Rab18 antibody (ab182764) at 1/400 dilution

All lanes:

Mouse heart tissue at 40 µg

Secondary

All lanes:

Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution

Predicted band size: 23 kDa

true

Exposure time: 1s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human Ras-related protein Rab-18 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

Q9NP72

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rab18 also known as RAB18A or RAB18 is a member of the Rab GTPase family a group of proteins involved in intracellular vesicle trafficking. Rab18 is approximately 23 kDa in mass. Researchers find Rab18 expressed widely in various tissues including the brain liver and adipose tissue. As a small GTPase Rab18 switches between an inactive GDP-bound state and an active GTP-bound state regulating membrane trafficking and fusion processes.

Biological function summary

The functions of Rab18 extend beyond simple vesicular transport. Rab18 interacts with lipid droplets and the endoplasmic reticulum influencing lipid metabolism and storage. It is sometimes present in a complex with proteins like TBC1D20 which acts as a GAP (GTPase-activating protein) that further regulates Rab18’s activity. Through these interactions Rab18 influences lipid homeostasis and endoplasmic reticulum organization adding a layer of control to cellular lipid balance.

Pathways

Rab18 participates in the regulation of lipid droplet growth and maturation linking it to lipid metabolism pathways. Its activity plays a role in the tethering of lipid droplets to the endoplasmic reticulum relating it functionally to proteins such as Seipin. Additionally Rab18 is involved in intracellular transport pathways where it cooperates with other Rab proteins like Rab3 and Rab10 to facilitate membrane trafficking and organelle dynamics.

Rab18 associations are evident in conditions like Warburg Micro syndrome and intellectual disability. Warburg Micro syndrome characterized by eye and brain abnormalities emerges from mutations affecting Rab18 or its interacting partners like RAB3GAP. Similarly Rab18’s involvement in lipid metabolism connects it with metabolic disorders where lipid storage and synthesis disruptions play a role. This links the functionality of Rab18 in cellular processes to broader impacts on human health.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes (PubMed : 24891604, PubMed : 30970241). Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (PubMed : 24891604, PubMed : 30970241). RAB18 is required for the localization of ZFYVE1 to lipid droplets and for its function in mediating the formation of endoplasmic reticulum-lipid droplets (ER-LD) contacts (PubMed : 30970241). Also required for maintaining endoplasmic reticulum structure (PubMed : 24891604). Plays a role in apical endocytosis/recycling (By similarity). Plays a key role in eye and brain development and neurodegeneration (PubMed : 21473985).

See full target information Ras-related protein Rab-18

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of addiction & prevention 13: PubMed40463894

2025

Alcohol, Anti-HIV Drugs, and/or Hippuric Acid Deteriorate Cellular Stresses in Senescent Hepatocytes and Aging Murine Liver.

Applications

Unspecified application

Species

Unspecified reactive species

L Chen,M Kaypaghian,E Duran,C Ji

Experimental and therapeutic medicine 24:476 PubMed35761814

2022

Hes-related family BHLH transcription factor with YRPW motif 1-activated proteasome 26S subunit, non-ATPase 14 regulates trophoblast function and endometrial angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Lingyun Zhang,Shan Zhang,Fangrong Chen

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Rab18 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab18 antibody (AB182764)

Western blot - Anti-Rab18 antibody (AB182764)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise