Rabbit Polyclonal RAB1B antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Ras-related protein Rab-1B aa 100 to C-terminus.
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Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human Ras-related protein Rab-1B aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H0U4
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
| Cow | Predicted |
| Cynomolgus monkey | Predicted |
| Orangutan | Predicted |
| Pig | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Pig, Cynomolgus monkey, Orangutan | Dilution info - | Notes - |
Target data
Function
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes (PubMed:20545908, PubMed:9437002). Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (PubMed:9437002). Plays a role in the initial events of the autophagic vacuole development which take place at specialized regions of the endoplasmic reticulum (PubMed:20545908). Regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments (By similarity). Required to modulate the compacted morphology of the Golgi (PubMed:26209634). Promotes the recruitment of lipid phosphatase MTMR6 to the endoplasmic reticulum-Golgi intermediate compartment (By similarity).
Alternative names
Ras-related protein Rab-1B, RAB1B
Recommended products
Rabbit Polyclonal RAB1B antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Ras-related protein Rab-1B aa 100 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human Ras-related protein Rab-1B aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H0U4
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity >955
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RAB1B also known as Ras-related protein Rab-1B is a member of the Rab family of small GTPases. This protein weighs approximately 22.6 kDa and is expressed in the cytoplasm. Mechanically RAB1B regulates vesicular trafficking between the endoplasmic reticulum (ER) and Golgi apparatus. This involves ensuring the proper budding movement and fusion of transport vesicles facilitating efficient transportation of proteins and lipids necessary for cellular function.
- Biological function summary
RAB1B plays a role in maintaining cellular homeostasis and protein secretion. It is an important component of the protein transport machinery participating in vesicle coat complex formation. It regulates the movement between the ER and Golgi working closely with other Rab proteins and their effectors to keep the flow of materials steady. The localization and activity of RAB1B influence processes important for cell growth and differentiation.
- Pathways
RAB1B operates significantly in the secretory and trafficking pathways. It connects with the Golgi apparatus and ER pathways participating in sorting and cellular signaling. RAB1B interacts with other proteins such as COPII and Golgi SNARE proteins to facilitate transport vesicle movement. These proteins and pathways ensure precise and organized intracellular cargo traffic impacting multiple cellular processes directly tied to the secretory pathway.
- Associated diseases and disorders
Disruptions in RAB1B function can connect to neurodegenerative diseases and cancers. Altered expression can affect protein transport linked to Alzheimer's disease where its dysregulation impacts ER-Golgi pathway proteins like APP. In various cancers abnormal RAB1B levels can influence proliferative signals and cellular stress responses. Understanding RAB1B's role helps to delineate its involvement in these diseases offering targets for therapeutic development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunocytochemistry/ Immunofluorescence - Anti-RAB1B antibody (ab234717)
U-251 (human brain glioma cell line) cells stained for RAB1B (green) using ab234717 at 1/100 dilution in ICC/IF. Secondary antibody is Alexa Fluor® 488-congugated Goat Anti-Rabbit IgG (H+L).
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