Rabbit Polyclonal RAB21 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RAB21 aa 50 to C-terminus.
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Publications
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- Cancer management and research 12:8833-88452020Long Non-Coding RNA GAS5 Suppresses Tumor Progression and Enhances the Radiosensitivity of Prostate Cancer Through the miR-320a/RAB21 Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiulong Ma et. al.PubMed 33061579
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human RAB21 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UL25
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/50.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Dog | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Dog | Dilution info - | Notes - |
Target data
Function
Small GTPase involved in membrane trafficking control (PubMed:18804435, PubMed:25648148). During the mitosis of adherent cells, controls the endosomal trafficking of integrins which is required for the successful completion of cytokinesis (PubMed:18804435). Regulates integrin internalization and recycling, but does not influence the traffic of endosomally translocated receptors in general (By similarity). As a result, may regulate cell adhesion and migration (By similarity). Involved in neurite growth (By similarity). Following SBF2/MTMT13-mediated activation in response to starvation-induced autophagy, binds to and regulates SNARE protein VAMP8 endolysosomal transport required for SNARE-mediated autophagosome-lysosome fusion (PubMed:25648148). Modulates protein levels of the cargo receptors TMED2 and TMED10, and required for appropriate Golgi localization of TMED10 (PubMed:31455601).
Alternative names
Ras-related protein Rab-21, RAB21, KIAA0118
Recommended products
Rabbit Polyclonal RAB21 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RAB21 aa 50 to C-terminus.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human RAB21 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UL25
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The RAB21 protein also known as Ras-related protein Rab-21 plays an important role in vesicle trafficking by regulating endosomal transport. It belongs to the Rab family of small GTPases which are important for vesicle movement and fusion. RAB21 has a molecular mass of approximately 24 kDa. This protein is widely expressed in various tissues including liver kidney and brain indicating its broad role in cellular processes. It localizes to cellular compartments associated with trafficking such as early endosomes and recycling endosomes.
- Biological function summary
Functions of RAB21 extend to roles in endocytic recycling and phagocytosis. It operates as part of cellular machinery that governs receptor-mediated endocytosis. RAB21 associates with integrins facilitating their proper endosomal transport and recycling back to the plasma membrane which is critical for cell adhesion and migration. By coordinating these activities RAB21 significantly influences cellular dynamics and structural integrity.
- Pathways
RAB21 impacts the regulation of integrin-mediated signaling and the endocytic pathway. It serves as a connector between the transport of integrins and downstream signaling events. RAB21 interacts with proteins such as Integrin β1 highlighting its integral role within the integrin trafficking pathway. This interaction highlights its influence in signaling cascades that dictate cell movement and adhesion further integrating into the network of cellular communication.
- Associated diseases and disorders
RAB21 is implicated in cancer and neurological disorders. Its dysfunction affects cancer cell migration and invasion due to improper integrin trafficking which is central to tumor progression and metastasis. In the domain of neurological disorders aberrant RAB21 activity is related to improper neural cell movement which affects development. Moreover RAB21's association with Integrin β1 suggests it influences both cancer and neural diseases highlighting its importance in maintaining cellular balance and function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB21 antibody (ab224390)
Paraffin-embedded human colon tissue stained for RAB21 using ab224390 at 1/20 dilution in immunohistochemical analysis.
Western blot - Anti-RAB21 antibody (ab224390)
All lanes: Western blot - Anti-RAB21 antibody (ab224390) at 1/100 dilution
Lane 1: Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2: RAB21 overexpression HEK-293T cell lysate (Co-expressed with a C-terminal myc-DDK tag, ~3.1 kDa)
Predicted band size: 24 kDa
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