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AB224390

Anti-RAB21 antibody

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(2 Publications)

Rabbit Polyclonal RAB21 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RAB21 aa 50 to C-terminus.

View Alternative Names

KIAA0118, RAB21, Ras-related protein Rab-21

1 Images
Western blot - Anti-RAB21 antibody (AB224390)
  • WB

Supplier Data

Western blot - Anti-RAB21 antibody (AB224390)

All lanes:

Western blot - Anti-RAB21 antibody (ab224390) at 1/100 dilution

Lane 1:

Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Lane 2:

RAB21 overexpression HEK-293T cell lysate (Co-expressed with a C-terminal myc-DDK tag, ~3.1 kDa)

Predicted band size: 24 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human RAB21 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UL25

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The RAB21 protein also known as Ras-related protein Rab-21 plays an important role in vesicle trafficking by regulating endosomal transport. It belongs to the Rab family of small GTPases which are important for vesicle movement and fusion. RAB21 has a molecular mass of approximately 24 kDa. This protein is widely expressed in various tissues including liver kidney and brain indicating its broad role in cellular processes. It localizes to cellular compartments associated with trafficking such as early endosomes and recycling endosomes.
Biological function summary

Functions of RAB21 extend to roles in endocytic recycling and phagocytosis. It operates as part of cellular machinery that governs receptor-mediated endocytosis. RAB21 associates with integrins facilitating their proper endosomal transport and recycling back to the plasma membrane which is critical for cell adhesion and migration. By coordinating these activities RAB21 significantly influences cellular dynamics and structural integrity.

Pathways

RAB21 impacts the regulation of integrin-mediated signaling and the endocytic pathway. It serves as a connector between the transport of integrins and downstream signaling events. RAB21 interacts with proteins such as Integrin β1 highlighting its integral role within the integrin trafficking pathway. This interaction highlights its influence in signaling cascades that dictate cell movement and adhesion further integrating into the network of cellular communication.

RAB21 is implicated in cancer and neurological disorders. Its dysfunction affects cancer cell migration and invasion due to improper integrin trafficking which is central to tumor progression and metastasis. In the domain of neurological disorders aberrant RAB21 activity is related to improper neural cell movement which affects development. Moreover RAB21's association with Integrin β1 suggests it influences both cancer and neural diseases highlighting its importance in maintaining cellular balance and function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (PubMed : 18804435, PubMed : 25648148, PubMed : 31455601). RAB21 is involved in membrane trafficking control (PubMed : 18804435, PubMed : 25648148). During the mitosis of adherent cells, controls the endosomal trafficking of integrins which is required for the successful completion of cytokinesis (PubMed : 18804435). Regulates integrin internalization and recycling, but does not influence the traffic of endosomally translocated receptors in general (By similarity). As a result, may regulate cell adhesion and migration (By similarity). Involved in neurite growth (By similarity). Following SBF2/MTMT13-mediated activation in response to starvation-induced autophagy, binds to and regulates SNARE protein VAMP8 endolysosomal transport required for SNARE-mediated autophagosome-lysosome fusion (PubMed : 25648148). Modulates protein levels of the cargo receptors TMED2 and TMED10, and required for appropriate Golgi localization of TMED10 (PubMed : 31455601).
See full target information RAB21
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 14:1182788 PubMed37089945

2023

L-carnitine prevents lenvatinib-induced muscle toxicity without impairment of the anti-angiogenic efficacy.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Jing,Tomohiro Iba,Hisamichi Naito,Pingping Xu,Jun-Ichi Morishige,Naoto Nagata,Hironao Okubo,Hitoshi Ando

Cancer management and research 12:8833-8845 PubMed33061579

2020

Long Non-Coding RNA GAS5 Suppresses Tumor Progression and Enhances the Radiosensitivity of Prostate Cancer Through the miR-320a/RAB21 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiulong Ma,Zhongwei Wang,Hongtao Ren,Xing Bao,Yang Zhang,Baofeng Wang,Dongli Ruan
View all publications
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Product promise

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