AB137093

Anti-RAB22A antibody [EPR9486]

RabMAb
Recombinant
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(17 Publications)

Rabbit Recombinant Monoclonal RAB22A antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 17 publications.

View Alternative Names

RAB22, RAB22A, Ras-related protein Rab-22A, Rab-22

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-RAB22A antibody [EPR9486] (AB137093)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RAB22A antibody [EPR9486] (AB137093)

Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling RAB22A with purified ab137093 at 1/100 dilution (7.6 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at 1/200 (2.5 μg/ml) dilution. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RAB22A antibody [EPR9486] (AB137093)

Immunocytochemistry/ Immunofluorescence analysis of MCF-7 cells labelling RAB22A with ab137093 (unpurified) at 1/250 dilution.

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Immunoprecipitation - Anti-RAB22A antibody [EPR9486] (AB137093)

ab137093 (purified) at 1/40 dilution (2ug) immunoprecipitating RAB22A in MCF7 whole cell lysates.
Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates 10ug
Lane 2 (+) : ab137093 & MCF7 whole cell lysates
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab137093 in MCF7 whole cell lysates
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-RAB22A antibody [EPR9486] (ab137093)

Predicted band size: 105 kDa,22 kDa

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Western blot - Anti-RAB22A antibody [EPR9486] (AB137093)

All lanes:

Western blot - Anti-RAB22A antibody [EPR9486] (ab137093) at 1/1000 dilution

Lane 1:

MCF-7 lysate at 10 µg

Lane 2:

Fetal kidney lysate at 10 µg

Lane 3:

BxPC-3 lysate at 10 µg

Lane 4:

Fetal heart lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 22 kDa

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Lab

Western blot - Anti-RAB22A antibody [EPR9486] (AB137093)

All lanes:

Western blot - Anti-RAB22A antibody [EPR9486] (ab137093) at 1/1000 dilution

All lanes:

Human brain lysates at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 22 kDa

Observed band size: 22 kDa

false

Lab

Western blot - Anti-RAB22A antibody [EPR9486] (AB137093)

All lanes:

Western blot - Anti-RAB22A antibody [EPR9486] (ab137093) at 1/1000 dilution

Lane 1:

MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Lane 2:

Mouse brain lysates at 15 µg

Lane 3:

Rat brain lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 22 kDa

Observed band size: 22 kDa

false

Carrier free

Anti-RAB22A antibody [EPR9486] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9486

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "For unpurified use at 1/250 - 1/500." }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAB22A also known as ras-related protein Rab-22A is a small GTPase with a molecular mass of approximately 21 kDa. It belongs to the Rab family of proteins that are involved in vesicular trafficking processes. Expressed in a variety of tissues RAB22A plays an important role in the regulation of endocytic pathways ensuring the proper sorting and trafficking of membrane proteins and lipids. Its expression has been noted prominently in the brain and other tissues indicating its widespread functional relevance.

Biological function summary

RAB22A regulates the early endosomal membrane traffic and acts as an important regulator of intracellular transport. This protein is involved in macropinocytosis and phagocytosis processes essential for cellular nutrient uptake and immune responses. RAB22A participates in protein complexes that modulate membrane fusion and fission impacting cellular dynamics and homeostasis. Through these activities it helps maintain efficient intracellular communication and transport.

Pathways

The function of RAB22A is integrated within the endocytic and recycling pathways. It interacts closely with proteins like RAB5 and RAB11 to facilitate the sorting and recycling of endosomes. These coordinative actions within the endosomal trafficking pathway are important for the maintenance of cellular functions including nutrient absorption and signal transduction. The interaction with these proteins highlights its significant role in the regulation of membrane and protein traffic.

RAB22A has implications for conditions such as cancer and neurodegenerative diseases. Its deregulation can lead to abnormal vesicle trafficking contributing to tumor progression by enhancing invasive characteristics of cancer cells. Additionally in neurodegenerative disorders improper RAB22A function can disrupt neuronal communication and contribute to disease pathology. Interactions with proteins like RAB7 and SNX27 further illuminate its role in disease processes offering potential targets for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes (PubMed : 16537905, PubMed : 21849477). Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (PubMed : 16537905). RAB22A plays a role in endocytosis and intracellular protein transport. Mediates trafficking of transferrin/TF from early endosomes to recycling endosomes (PubMed : 16537905). Required for NGF-mediated endocytosis of NTRK1, and subsequent neurite outgrowth (PubMed : 21849477). Has low GTPase activity (PubMed : 16537905).

See full target information RAB22A

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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Stem cell research & therapy 16:39 PubMed39901252

2025

Enhanced therapeutic effects of hypoxia-preconditioned mesenchymal stromal cell-derived extracellular vesicles in renal ischemic injury.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Yuan,Jie Liu,Liang Zhong,Pengtao Liu,Ting Li,Kexin Yang,Wei Gao,Guangyuan Zhang,Jie Sun,Xiangyu Zou

Journal of extracellular vesicles 13:e12494 PubMed39051763

2024

RAB22A sorts epithelial growth factor receptor (EGFR) from early endosomes to recycling endosomes for microvesicles release.

Applications

Unspecified application

Species

Unspecified reactive species

Yujie Lin,Denghui Wei,Xiaobo He,Lanqing Huo,Jingxuan Wang,Xia Zhang,Yuanzhong Wu,Ruhua Zhang,Ying Gao,Tiebang Kang

Molecular biotechnology 67:2357-2366 PubMed38850457

2024

Linc00239 Facilitates the Progress of Clear Cell Renal Cell Carcinoma via the miR-204-5p/RAB22A Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Cheng,Shuangquan Lin,Anyi Zhu,Zhengdong Hong,Zimin Shi,Huanhuan Deng,Gan Zhang

Acta biochimica et biophysica Sinica 54:1897-1908 PubMed36789688

2023

pPe p inhibits HGC-27 cell proliferation, migration and invasion by upregulating miR-30b-5p and down-regulating the Rac1/Cdc42 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjun Xu,Zhenjie Fu,Yuqin Xu,Man Hei Cheung,Yan Chen,Meiai Lin,Hang Wen,Hang Lv,Chun Liang,Jianshu Lou,Yitao Chen

Cell research 32:1086-1104 PubMed36280710

2022

Intercellular transfer of activated STING triggered by RAB22A-mediated non-canonical autophagy promotes antitumor immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Gao,Xueping Zheng,Boyang Chang,Yujie Lin,Xiaodan Huang,Wen Wang,Shirong Ding,Weixiang Zhan,Shang Wang,Beibei Xiao,Lanqing Huo,Youhui Yu,Yilin Chen,Run Gong,Yuanzhong Wu,Ruhua Zhang,Li Zhong,Xin Wang,Qiuyan Chen,Song Gao,Zhengfan Jiang,Denghui Wei,Tiebang Kang

Thoracic cancer 13:571-581 PubMed34989145

2022

Circ_0007385 regulates cell proliferation, apoptosis and stemness via targeting miR-493-3p/RAB22A axis in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Dongxiao Ding,Feng Yang,Zhongjie Chen,Junjie Ying

Frontiers in cell and developmental biology 9:653207 PubMed34012964

2021

Circular RNA Circ0021205 Promotes Cholangiocarcinoma Progression Through MiR-204-5p/RAB22A Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jianfei Tu,Weiqian Chen,Liyun Zheng,Shiji Fang,Dengke Zhang,Chunli Kong,Yang Yang,Rongfang Qiu,Zhongwei Zhao,Chenying Lu,Xiaojie Lu,Jiansong Ji

Small GTPases 12:282-293 PubMed32281471

2020

Rab22a regulates the establishment of epithelial polarity.

Applications

Unspecified application

Species

Unspecified reactive species

Isabella R Blum,Caroline Behling-Hess,Marco Padilla-Rodriguez,Samina Momtaz,Christopher Cox,Jean M Wilson

International journal of cardiology 315:72-80 PubMed32349937

2020

LncRNA ZFAS1 confers inflammatory responses and reduces cholesterol efflux in atherosclerosis through regulating miR-654-3p-ADAM10/RAB22A axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoqiang Tang,Ruohan Yin,Haifeng Shi,Xiang Wang,Dong Shen,Xiaoqin Wang,Changjie Pan

Cell reports 30:2444-2459.e7 PubMed32075774

2020

CtBP1-Mediated Membrane Fission Contributes to Effective Recycling of Synaptic Vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Daniela Ivanova,Cordelia Imig,Marcial Camacho,Annika Reinhold,Debarpan Guhathakurta,Carolina Montenegro-Venegas,Michael A Cousin,Eckart D Gundelfinger,Christian Rosenmund,Benjamin Cooper,Anna Fejtova

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RAB22A antibody [EPR9486]

Immunocytochemistry/ Immunofluorescence - Anti-RAB22A antibody [EPR9486] (AB137093)

Immunocytochemistry/ Immunofluorescence - Anti-RAB22A antibody [EPR9486] (AB137093)

Immunoprecipitation - Anti-RAB22A antibody [EPR9486] (AB137093)

Western blot - Anti-RAB22A antibody [EPR9486] (AB137093)

Western blot - Anti-RAB22A antibody [EPR9486] (AB137093)

Western blot - Anti-RAB22A antibody [EPR9486] (AB137093)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (17)

Product promise