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Rabbit Recombinant Monoclonal RAB22A antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 13 publications.

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Images

Western blot - Anti-RAB22A antibody [EPR9486] (AB137093), expandable thumbnail
  • Immunoprecipitation - Anti-RAB22A antibody [EPR9486] (AB137093), expandable thumbnail
  • Western blot - Anti-RAB22A antibody [EPR9486] (AB137093), expandable thumbnail
  • Western blot - Anti-RAB22A antibody [EPR9486] (AB137093), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-RAB22A antibody [EPR9486] (AB137093), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IF
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Rat
Expected
Tested
Expected

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

For unpurified use at 1/250 - 1/500.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Plays a role in endocytosis and intracellular protein transport. Mediates trafficking of TF from early endosomes to recycling endosomes (PubMed:16537905). Required for NGF-mediated endocytosis of NTRK1, and subsequent neurite outgrowth (PubMed:21849477). Binds GTP and GDP and has low GTPase activity. Alternates between a GTP-bound active form and a GDP-bound inactive form (PubMed:16537905).

Alternative names

RAB22, RAB22A, Ras-related protein Rab-22A, Rab-22

Recommended products

Rabbit Recombinant Monoclonal RAB22A antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 13 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR9486
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

RAB22A also known as ras-related protein Rab-22A is a small GTPase with a molecular mass of approximately 21 kDa. It belongs to the Rab family of proteins that are involved in vesicular trafficking processes. Expressed in a variety of tissues RAB22A plays an important role in the regulation of endocytic pathways ensuring the proper sorting and trafficking of membrane proteins and lipids. Its expression has been noted prominently in the brain and other tissues indicating its widespread functional relevance.

Biological function summary

RAB22A regulates the early endosomal membrane traffic and acts as an important regulator of intracellular transport. This protein is involved in macropinocytosis and phagocytosis processes essential for cellular nutrient uptake and immune responses. RAB22A participates in protein complexes that modulate membrane fusion and fission impacting cellular dynamics and homeostasis. Through these activities it helps maintain efficient intracellular communication and transport.

Pathways

The function of RAB22A is integrated within the endocytic and recycling pathways. It interacts closely with proteins like RAB5 and RAB11 to facilitate the sorting and recycling of endosomes. These coordinative actions within the endosomal trafficking pathway are important for the maintenance of cellular functions including nutrient absorption and signal transduction. The interaction with these proteins highlights its significant role in the regulation of membrane and protein traffic.

Associated diseases and disorders

RAB22A has implications for conditions such as cancer and neurodegenerative diseases. Its deregulation can lead to abnormal vesicle trafficking contributing to tumor progression by enhancing invasive characteristics of cancer cells. Additionally in neurodegenerative disorders improper RAB22A function can disrupt neuronal communication and contribute to disease pathology. Interactions with proteins like RAB7 and SNX27 further illuminate its role in disease processes offering potential targets for therapeutic intervention.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-RAB22A antibody [EPR9486] (ab137093), expandable thumbnail

    Western blot - Anti-RAB22A antibody [EPR9486] (ab137093)

    All lanes: Western blot - Anti-RAB22A antibody [EPR9486] (ab137093) at 1/1000 dilution

    Lane 1: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 15 µg

    Lane 2: Mouse brain lysates at 15 µg

    Lane 3: Rat brain lysates at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 22 kDa

    Observed band size: 22 kDa

  • Immunoprecipitation - Anti-RAB22A antibody [EPR9486] (ab137093), expandable thumbnail

    Immunoprecipitation - Anti-RAB22A antibody [EPR9486] (ab137093)

    ab137093 (purified) at 1/40 dilution (2ug) immunoprecipitating RAB22A in MCF7 whole cell lysates.
    Lane 1: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates 10ug
    Lane 2 (+): ab137093 & MCF7 whole cell lysates
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab137093 in MCF7 whole cell lysates
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-RAB22A antibody [EPR9486] (ab137093)

    Predicted band size: 105 kDa, 22 kDa

  • Western blot - Anti-RAB22A antibody [EPR9486] (ab137093), expandable thumbnail

    Western blot - Anti-RAB22A antibody [EPR9486] (ab137093)

    All lanes: Western blot - Anti-RAB22A antibody [EPR9486] (ab137093) at 1/1000 dilution

    All lanes: Human brain lysates at 15 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 22 kDa

    Observed band size: 22 kDa

  • Western blot - Anti-RAB22A antibody [EPR9486] (ab137093), expandable thumbnail

    Western blot - Anti-RAB22A antibody [EPR9486] (ab137093)

    All lanes: Western blot - Anti-RAB22A antibody [EPR9486] (ab137093) at 1/1000 dilution

    Lane 1: MCF-7 lysate at 10 µg

    Lane 2: Fetal kidney lysate at 10 µg

    Lane 3: BxPC-3 lysate at 10 µg

    Lane 4: Fetal heart lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 22 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-RAB22A antibody [EPR9486] (ab137093), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-RAB22A antibody [EPR9486] (ab137093)

    Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling RAB22A with purified ab137093 at 1/100 dilution (7.6 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 μg/ml) dilution. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunocytochemistry/ Immunofluorescence - Anti-RAB22A antibody [EPR9486] (ab137093), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-RAB22A antibody [EPR9486] (ab137093)

    Immunocytochemistry/ Immunofluorescence analysis of MCF-7 cells labelling RAB22A with ab137093 (unpurified) at 1/250 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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