Rabbit Recombinant Monoclonal RAB22A antibody. Carrier free. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples.
Images
![Immunoprecipitation - Anti-RAB22A antibody [EPR9486] - BSA and Azide free (AB240105), expandable thumbnail](https://content.abcam.com/products/images/rab22a-antibody-epr9486-bsa-and-azide-free-ab240105--immunoprecipitation-img4631.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-RAB22A antibody [EPR9486] - BSA and Azide free (AB240105)")
![Immunocytochemistry/ Immunofluorescence - Anti-RAB22A antibody [EPR9486] - BSA and Azide free (AB240105), expandable thumbnail](https://content.abcam.com/products/images/rab22a-antibody-epr9486-bsa-and-azide-free-ab240105--immunocytochemistry-immunofluorescence-img18686.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-RAB22A antibody [EPR9486] - BSA and Azide free (AB240105)")
![Immunocytochemistry/ Immunofluorescence - Anti-RAB22A antibody [EPR9486] - BSA and Azide free (AB240105), expandable thumbnail](https://content.abcam.com/products/images/rab22a-antibody-epr9486-bsa-and-azide-free-ab240105--immunocytochemistry-immunofluorescence-img108249.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-RAB22A antibody [EPR9486] - BSA and Azide free (AB240105)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Mouse | Predicted | Expected | Predicted |
| Rat | Predicted | Expected | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Plays a role in endocytosis and intracellular protein transport. Mediates trafficking of TF from early endosomes to recycling endosomes (PubMed:16537905). Required for NGF-mediated endocytosis of NTRK1, and subsequent neurite outgrowth (PubMed:21849477). Binds GTP and GDP and has low GTPase activity. Alternates between a GTP-bound active form and a GDP-bound inactive form (PubMed:16537905).
Alternative names
RAB22, RAB22A, Ras-related protein Rab-22A, Rab-22
Recommended products
Rabbit Recombinant Monoclonal RAB22A antibody. Carrier free. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR9486
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab240105 is the carrier-free version of Anti-RAB22A antibody [EPR9486] ab137093.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RAB22A also known as ras-related protein Rab-22A is a small GTPase with a molecular mass of approximately 21 kDa. It belongs to the Rab family of proteins that are involved in vesicular trafficking processes. Expressed in a variety of tissues RAB22A plays an important role in the regulation of endocytic pathways ensuring the proper sorting and trafficking of membrane proteins and lipids. Its expression has been noted prominently in the brain and other tissues indicating its widespread functional relevance.
- Biological function summary
RAB22A regulates the early endosomal membrane traffic and acts as an important regulator of intracellular transport. This protein is involved in macropinocytosis and phagocytosis processes essential for cellular nutrient uptake and immune responses. RAB22A participates in protein complexes that modulate membrane fusion and fission impacting cellular dynamics and homeostasis. Through these activities it helps maintain efficient intracellular communication and transport.
- Pathways
The function of RAB22A is integrated within the endocytic and recycling pathways. It interacts closely with proteins like RAB5 and RAB11 to facilitate the sorting and recycling of endosomes. These coordinative actions within the endosomal trafficking pathway are important for the maintenance of cellular functions including nutrient absorption and signal transduction. The interaction with these proteins highlights its significant role in the regulation of membrane and protein traffic.
- Associated diseases and disorders
RAB22A has implications for conditions such as cancer and neurodegenerative diseases. Its deregulation can lead to abnormal vesicle trafficking contributing to tumor progression by enhancing invasive characteristics of cancer cells. Additionally in neurodegenerative disorders improper RAB22A function can disrupt neuronal communication and contribute to disease pathology. Interactions with proteins like RAB7 and SNX27 further illuminate its role in disease processes offering potential targets for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunoprecipitation - Anti-RAB22A antibody [EPR9486] - BSA and Azide free (ab240105), expandable thumbnail](https://content.abcam.com/products/images/rab22a-antibody-epr9486-bsa-and-azide-free-ab240105--immunoprecipitation-img4631.jpg "Immunoprecipitation - Anti-RAB22A antibody [EPR9486] - BSA and Azide free (ab240105)")
Immunoprecipitation - Anti-RAB22A antibody [EPR9486] - BSA and Azide free (ab240105)
Anti-RAB22A antibody [EPR9486] ab137093 (purified) at 1/40 dilution (2ug) immunoprecipitating RAB22A in MCF7 whole cell lysates.
Lane 1: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates 10ug
Lane 2 (+): Anti-RAB22A antibody [EPR9486] ab137093 & MCF7 whole cell lysates
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-RAB22A antibody [EPR9486] ab137093 in MCF7 whole cell lysates
For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RAB22A antibody [EPR9486] ab137093).
All lanes: Immunoprecipitation - Anti-RAB22A antibody [EPR9486] (Anti-RAB22A antibody [EPR9486] ab137093)
Predicted band size: 105 kDa, 22 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-RAB22A antibody [EPR9486] - BSA and Azide free (ab240105), expandable thumbnail](https://content.abcam.com/products/images/rab22a-antibody-epr9486-bsa-and-azide-free-ab240105--immunocytochemistry-immunofluorescence-img18686.jpg "Immunocytochemistry/ Immunofluorescence - Anti-RAB22A antibody [EPR9486] - BSA and Azide free (ab240105)")
Immunocytochemistry/ Immunofluorescence - Anti-RAB22A antibody [EPR9486] - BSA and Azide free (ab240105)
Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling RAB22A with purified Anti-RAB22A antibody [EPR9486] ab137093 at 1/100 dilution (7.6 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 μg/ml) dilution. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RAB22A antibody [EPR9486] ab137093).
![Immunocytochemistry/ Immunofluorescence - Anti-RAB22A antibody [EPR9486] - BSA and Azide free (ab240105), expandable thumbnail](https://content.abcam.com/products/images/rab22a-antibody-epr9486-bsa-and-azide-free-ab240105--immunocytochemistry-immunofluorescence-img108249.jpg "Immunocytochemistry/ Immunofluorescence - Anti-RAB22A antibody [EPR9486] - BSA and Azide free (ab240105)")
Immunocytochemistry/ Immunofluorescence - Anti-RAB22A antibody [EPR9486] - BSA and Azide free (ab240105)
Immunocytochemistry/ Immunofluorescence analysis of MCF-7 cells labelling RAB22A with Anti-RAB22A antibody [EPR9486] ab137093 (unpurified) at 1/250 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RAB22A antibody [EPR9486] ab137093).
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