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AB240105

Anti-RAB22A antibody [EPR9486] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal RAB22A antibody. Carrier free. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

RAB22, RAB22A, Ras-related protein Rab-22A, Rab-22

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-RAB22A antibody [EPR9486] - BSA and Azide free (AB240105)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RAB22A antibody [EPR9486] - BSA and Azide free (AB240105)

Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling RAB22A with purified ab137093 at 1/100 dilution (7.6 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 μg/ml) dilution. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137093).

Immunocytochemistry/ Immunofluorescence - Anti-RAB22A antibody [EPR9486] - BSA and Azide free (AB240105)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RAB22A antibody [EPR9486] - BSA and Azide free (AB240105)

Immunocytochemistry/ Immunofluorescence analysis of MCF-7 cells labelling RAB22A with ab137093 (unpurified) at 1/250 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137093).

Immunoprecipitation - Anti-RAB22A antibody [EPR9486] - BSA and Azide free (AB240105)
  • IP

Unknown

Immunoprecipitation - Anti-RAB22A antibody [EPR9486] - BSA and Azide free (AB240105)

ab137093 (purified) at 1/40 dilution (2ug) immunoprecipitating RAB22A in MCF7 whole cell lysates.
Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates 10ug
Lane 2 (+) : ab137093 & MCF7 whole cell lysates
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab137093 in MCF7 whole cell lysates
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137093).

All lanes:

Immunoprecipitation - Anti-RAB22A antibody [EPR9486] (<a href='/en-us/products/primary-antibodies/rab22a-antibody-epr9486-ab137093'>ab137093</a>)

Predicted band size: 105 kDa,22 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9486

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IP, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab240105 is the carrier-free version of ab137093.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAB22A also known as ras-related protein Rab-22A is a small GTPase with a molecular mass of approximately 21 kDa. It belongs to the Rab family of proteins that are involved in vesicular trafficking processes. Expressed in a variety of tissues RAB22A plays an important role in the regulation of endocytic pathways ensuring the proper sorting and trafficking of membrane proteins and lipids. Its expression has been noted prominently in the brain and other tissues indicating its widespread functional relevance.
Biological function summary

RAB22A regulates the early endosomal membrane traffic and acts as an important regulator of intracellular transport. This protein is involved in macropinocytosis and phagocytosis processes essential for cellular nutrient uptake and immune responses. RAB22A participates in protein complexes that modulate membrane fusion and fission impacting cellular dynamics and homeostasis. Through these activities it helps maintain efficient intracellular communication and transport.

Pathways

The function of RAB22A is integrated within the endocytic and recycling pathways. It interacts closely with proteins like RAB5 and RAB11 to facilitate the sorting and recycling of endosomes. These coordinative actions within the endosomal trafficking pathway are important for the maintenance of cellular functions including nutrient absorption and signal transduction. The interaction with these proteins highlights its significant role in the regulation of membrane and protein traffic.

RAB22A has implications for conditions such as cancer and neurodegenerative diseases. Its deregulation can lead to abnormal vesicle trafficking contributing to tumor progression by enhancing invasive characteristics of cancer cells. Additionally in neurodegenerative disorders improper RAB22A function can disrupt neuronal communication and contribute to disease pathology. Interactions with proteins like RAB7 and SNX27 further illuminate its role in disease processes offering potential targets for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes (PubMed : 16537905, PubMed : 21849477). Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (PubMed : 16537905). RAB22A plays a role in endocytosis and intracellular protein transport. Mediates trafficking of transferrin/TF from early endosomes to recycling endosomes (PubMed : 16537905). Required for NGF-mediated endocytosis of NTRK1, and subsequent neurite outgrowth (PubMed : 21849477). Has low GTPase activity (PubMed : 16537905).
See full target information RAB22A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature 644:790-798 PubMed40533564

2025

Kupffer cell programming by maternal obesity triggers fatty liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Huang,Nora R Balzer,Lea Seep,Iva Splichalova,Nelli Blank-Stein,Maria Francesca Viola,Eliana Franco Taveras,Kerim Acil,Diana Fink,Franzisca Petrovic,Nikola Makdissi,Seyhmus Bayar,Katharina Mauel,Carolin Radwaniak,Jelena Zurkovic,Amir H Kayvanjoo,Klaus Wunderling,Malin Jessen,Mohamed H Yaghmour,Lukas Kenner,Thomas Ulas,Stephan Grein,Joachim L Schultze,Charlotte L Scott,Martin Guilliams,Zhaoyuan Liu,Florent Ginhoux,Marc D Beyer,Christoph Thiele,Felix Meissner,Jan Hasenauer,Dagmar Wachten,Elvira Mass
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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