Rabbit Polyclonal RAB23 antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human RAB23.
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- Oncology letters 15:5155-51602018Rab23 promotes the cisplatin resistance of ovarian cancer via the Shh-Gli-ABCG2 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenjie Zhang et. al.PubMed 29552151
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human RAB23. The exact immunogen used to generate this antibody is proprietary information. Database link Q9ULC3
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5 | Notes - |
Species Mouse | Dilution info 1/5 | Notes - |
Target data
Function
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. Together with SUFU, prevents nuclear import of GLI1, and thereby inhibits GLI1 transcription factor activity. Regulates GLI1 in differentiating chondrocytes. Likewise, regulates GLI3 proteolytic processing and modulates GLI2 and GLI3 transcription factor activity. Plays a role in autophagic vacuole assembly, and mediates defense against pathogens, such as S.aureus, by promoting their capture by autophagosomes that then merge with lysosomes.
Alternative names
HSPC137, RAB23, Ras-related protein Rab-23
Recommended products
Rabbit Polyclonal RAB23 antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human RAB23.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human RAB23. The exact immunogen used to generate this antibody is proprietary information. Database link Q9ULC3
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Purity is > 95% (by SDS-PAGE).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RAB23 also known as Ras-related protein Rab-23 is a small GTPase of the RAB family. It has a molecular weight of approximately 25 kDa. This protein is expressed in various tissues with significant expression in the brain skin and kidney. RAB23 acts as a molecular switch by cycling between inactive GDP-bound and active GTP-bound states. It regulates intracellular vesicle trafficking influencing the movement and fusion of vesicles within the cell.
- Biological function summary
RAB23 plays an important role in modulating various cellular processes such as intracellular transport and signal transduction. It interacts with effector proteins through its active GTP-bound form which allows it to facilitate certain cellular functions. RAB23 is known to associate with specific protein complexes contributing to pathways that affect cellular movement and organization reflecting its importance in cellular dynamics.
- Pathways
RAB23 is a part of the Hedgehog signaling pathway involved in embryonic development and cell differentiation. Within this pathway RAB23 regulates the activity of proteins like GLI transcription factors to control gene expression important for developmental processes. Additionally RAB23 interacts with other GTPases linking to the actin cytoskeleton remodeling pathway which impacts cellular structure and polarity.
- Associated diseases and disorders
RAB23 has been linked to certain congenital conditions such as Carpenter syndrome which involves malformations in the skull and limbs. Mutations in the RAB23 gene can disrupt normal Hedgehog signaling leading to developmental defects. Furthermore the protein's association with GLI transcription factors connects it to various developmental disorders marked by alterations in early patterning and organ formation. Such links underline the significance of RAB23 in developmental biology and its potential as a therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-RAB23 antibody (ab192420)
All lanes: Western blot - Anti-RAB23 antibody (ab192420)
Lane 1: MCF7 whole cell lysate
Lane 2: HEK293T whole cell lysate
Lane 3: NIH 3T3 whole cell lysate
Predicted band size: 27 kDa
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