AB223044

Anti-RAB27A antibody

Name: Anti-RAB27A antibody (ab223044) | Abcam
Brand: Abcam Limited
SKU: AB223044
Price: 525 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(4 Publications)

Rabbit Polyclonal RAB27A antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RAB27A.

View Alternative Names

RAB27, RAB27A, Ras-related protein Rab-27A, Rab-27, GTP-binding protein Ram

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB27A antibody (AB223044)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB27A antibody (AB223044)

Paraffin-embedded human pancreatic tissue stained for RAB27A using ab223044 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB27A antibody (AB223044)

Paraffin-embedded human spleen tissue stained for RAB27A using ab223044 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-RAB27A antibody (AB223044)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RAB27A antibody (AB223044)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for RAB27A (green) using ab223044 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488^® congugated Goat Anti-Rabbit IgG (H+L).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human RAB27A.

P51159

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%.

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAB27A also known as ras-related protein Rab-27A is a small GTPase that plays a role in intracellular vesicle trafficking. It has a molecular mass of approximately 25 kDa. RAB27A localizes in many cell types with higher expressions in secretory cells such as melanocytes and cytotoxic T lymphocytes. It primarily functions in the regulation of vesicle docking and fusion processes with target membranes facilitating the transport of contents such as enzymes and other proteins to specific sites within the cell.

Biological function summary

This protein regulates the exocytosis processes in certain secretory cells. RAB27A partners with effectors through its GDP-bound and GTP-bound states forming protein complexes to mediate the movement and anchoring of secretory granules. Melanocytes depend on RAB27A to control the distribution of melanosomes which are organelles containing pigment melanin. In immune cells RAB27A regulates the secretion of lytic granules significantly impacting the immune response.

Pathways

This protein is involved in the melanogenesis pathway and the lytic granule exocytosis pathway. In the melanogenesis pathway RAB27A works closely with proteins such as Melanophilin and Myosin Va to control melanosome transport. In the exocytosis pathway in immune cells RAB27A interacts with proteins like UNC13D which are vital for the release of cytotoxic molecules. By controlling these pathways RAB27A influences pigment formation in skin and hair as well as immune defense mechanisms.

Mutations in the RAB27A gene are associated with Griscelli syndrome type 2 a disorder characterized by partial albinism and immune system defects. This syndrome arises from impaired melanosome transport due to dysfunctional RAB27A and its associated proteins such as Melanophilin and Myosin Va. Additionally defects in RAB27A also relate to hemophagocytic lymphohistiocytosis type 2 where impaired function in cytotoxic T lymphocytes reduces immune response effectiveness leading to excessive inflammation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (PubMed : 30771381). RAB27A regulates homeostasis of late endocytic pathway, including endosomal positioning, maturation and secretion (PubMed : 30771381). Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse (PubMed : 18812475).

See full target information RAB27A

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of physiology and pharmacology : an official journal of the Polish Physiological Society 75:205-213 PubMed38736267

2024

Campothecin suppresses cell proliferation and migration in head and neck squamous cell carcinoma by blocking RAB27A-mediated phosphatidylinositol 3 kinase (PI3K)/protein kinase B (AKT) pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Y Zhao,Y Wang,L Zhao,L Qu,J H Zheng

Journal of cell science 136: PubMed37039765

2023

Activity-dependent post-translational regulation of palmitoylating and depalmitoylating enzymes in the hippocampus.

Applications

Unspecified application

Species

Unspecified reactive species

Danya Abazari,Angela R Wild,Tian Qiu,Bryan C Dickinson,Shernaz X Bamji

International journal of molecular sciences 20: PubMed31795513

2019

A Paradigm in Immunochemistry, Revealed by Monoclonal Antibodies to Spatially Distinct Epitopes on Syntenin-1.

Applications

Unspecified application

Species

Unspecified reactive species

Ian R D Johnson,Alexandra Sorvina,Jessica M Logan,Courtney R Moore,Jessica K Heatlie,Emma J Parkinson-Lawrence,Stavros Selemidis,John J O'Leary,Lisa M Butler,Douglas A Brooks

Journal of cellular physiology : PubMed30723916

2019

Blockage of transferred exosome-shuttled miR-494 inhibits melanoma growth and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Li,Jia Chen,Shaohua Wang,Ping Li,Changli Zheng,Xiao Zhou,Yongguang Tao,Xiang Chen,Lichun Sun,Aijun Wang,Ke Cao,Shijie Tang,Jianda Zhou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RAB27A antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB27A antibody (AB223044)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB27A antibody (AB223044)

Immunocytochemistry/ Immunofluorescence - Anti-RAB27A antibody (AB223044)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise