Anti-RAB27A + RAB27B antibody [1G7]

7 product images

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  Western blot - Anti-RAB27A + RAB27B antibody [1G7] (ab55667)

  RAB27A + RAB27B Western blot staining using mouse Anti-RAB27A + RAB27B antibody

  Lane 1: Wild type HAP1 whole cell lysate (20 μg)
  Lane 2: RAB27A knockout HAP1 whole cell lysate (20 μg)
  Lane 3: Jurkat whole cell lysate (20 μg)
  Lane 4: MCF7 whole cell lysate (20 μg)
  

  Lanes 1 - 4: Merged signal (red and green). Green - ab55667 observed at 27 kDa. Red - loading control, Anti-GAPDH antibody [EPR16891] - Loading Control ab181602, observed at 37 kDa.

  ab55667 was shown to specifically react with RAB27A when RAB27A knockout samples were used. Wild-type and RAB27A knockout samples were subjected to SDS-PAGE. ab55667 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 2.5 ug/ml and 1/10000 dilution respectively. Blots were developed with IRDye^® 800CW Goat anti-Mouse IgG (H + L) and IRDye^® 680 Goat anti-Rabbit IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  This image was generated using the ascites version of the product.

  All lanes: Western blot - Anti-RAB27A + RAB27B antibody [1G7] (ab55667)

  Predicted band size: 25 kDa

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  Western blot - Anti-RAB27A + RAB27B antibody [1G7] (ab55667)

  RAB27A + RAB27B Western blot staining of HL-60 cell lysate using mouse Anti-RAB27A + RAB27B antibody

  This image was generated using the ascites version of the product.

  All lanes: Western blot - Anti-RAB27A + RAB27B antibody [1G7] (ab55667)

  All lanes: HL-60 cell lysate

  Predicted band size: 25 kDa

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  Western blot - Anti-RAB27A + RAB27B antibody [1G7] (ab55667)

  RAB27A + RAB27B Western blot staining using mouse Anti-RAB27A + RAB27B antibody

  GAPDH (36.1 kDa) used as specificity and loading control.
  

  This image was generated using the ascites version of the product.

  All lanes: Western blot - Anti-RAB27A + RAB27B antibody [1G7] (ab55667)

  Lane 1: RAB27A over-expressed 293 cell line, cotransfected with RAB27A Validated Chimera RNAi

  Lane 2: Non-transfected control

  Predicted band size: 25 kDa

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  Western blot - Anti-RAB27A + RAB27B antibody [1G7] (ab55667)

  RAB27A + RAB27B Western blot staining using mouse Anti-RAB27A + RAB27B antibody

  This image was generated using the ascites version of the product.

  All lanes: Western blot - Anti-RAB27A + RAB27B antibody [1G7] (ab55667)

  Lane 1: RAB27A transfected lysate(24.9 KDa)

  Lane 2: Non-transfected lysate

  Predicted band size: 25 kDa, 41 kDa

  Observed band size: 38 kDa

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  This image is courtesy of a customer review submitted by Dr Vladimir Milenkovic

  Western blot - Anti-RAB27A + RAB27B antibody [1G7] (ab55667)

  RAB27A + RAB27B Western blot staining using mouse Anti-RAB27A + RAB27B antibody

  Rab27a (25kDa)+GFP(27kDa)=52kDa
  

  The blot was blocked with 5% milk for 30 minutes at 25°C and incubated with the primary antibody for 12 hours at 4°C.

  This image was generated using the ascites version of the product.

  All lanes: Western blot - Anti-RAB27A + RAB27B antibody [1G7] (ab55667) at 1/200 dilution

  Lane 1: whole cell lysate of HEK 293 transfected with empty pCDNA3 vector at 20 µg

  Lane 2: whole cell lysate of HEK 293 cells expressing Rab27a-GFP at 20 µg

  Secondary

  All lanes: HRP conjugated goat anti-mouse

  Developed using the ECL technique.

  Predicted band size: 25 kDa

  Observed band size: 52 kDa

  Exposure time: 30min

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB27A + RAB27B antibody [1G7] (ab55667)

  RAB27A + RAB27B Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-RAB27A + RAB27B antibody

  Anti-RAB27A + RAB27B antibody [1G7] (ab55667) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human lymphoma. Heat induced antigen retrieval with Citrate buffer pH 6 has been performed prior to immunostaining.
  

  This image was generated using the ascites version of the product.

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  Western blot - Anti-RAB27A + RAB27B antibody [1G7] (ab55667)

  RAB27A + RAB27B Western blot staining using mouse Anti-RAB27A + RAB27B antibody

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.
  Anti-Flag tag antibody was used as a loading control at a 1/100,000 dilution.
  Anti-6X His tag® antibody [EPR20547] - ChIP Grade, Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204 was used at 1/5,000 dilution.

  All lanes: Western blot - Anti-RAB27A + RAB27B antibody [1G7] (ab55667) at 1/1000 dilution

  Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with an empty vector contain a His-tag whole cell lysate at 20 µg

  Lane 2: 293T cells transfected with a human RAB27A expression vector containi a His-tag whole cell lysate at 20 µg

  Lane 3: 293T cells transfected with a human RAB27B expression vector containi a Flag-tag whole cell lysate at 20 µg

  Secondary

  Lanes 1 - 3: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution

  Lanes 1 - 3: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/20000 dilution

  Observed band size: 27 kDa

  Exposure time: 1s