Sample Prep & Detection Kits
Conjugation kitsPurification kitsSample preparation kitsChromogen kitsIHC kitsChIP kitsAccessory Reagents & Controls
Accessory reagents & controlsBiochemicals
BiochemicalsProteins and Peptides
Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
Learn about all product ranges with our product overviews.
Featured events
Make new connections at our global events.
Our programs
New Lab Program
Get a head start with our exclusive new lab discount. Enjoy 20% off and free shipping for three months.
New Biotech Program
Just starting out? Get 15% off and free shipping to your lab for six months.
Product promise
Peace of mind that all products perform as stated.
Product reviews
Leave reviews, get rewarded and help your community.
Trial program
Try untested species and applications to earn money off your next order.
Product Insider Program
Be the first to know about our latest product launches - and unlock exclusive offers and discounts.
Rabbit Recombinant Monoclonal RAB27A antibody. Suitable for IHC-P, WB, IP, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat, Transfected cell lysate - Human, Transfected cell line - Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
IHC-P | WB | IP | ICC/IF | Flow Cyt (Intra) | |
---|---|---|---|---|---|
Human | Tested | Tested | Tested | Tested | Tested |
Mouse | Not recommended | Tested | Not recommended | Expected | Expected |
Rat | Not recommended | Tested | Expected | Expected | Expected |
Transfected cell line - Human | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
Transfected cell lysate - Human | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/5000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Transfected cell lysate - Human, Transfected cell line - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species Transfected cell lysate - Human | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell line - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/30 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Transfected cell lysate - Human, Transfected cell line - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 | Notes - |
Species Transfected cell line - Human | Dilution info 1/100 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell lysate - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell lysate - Human, Transfected cell line - Human | Dilution info - | Notes - |
Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate homeostasis of late endocytic pathway, including endosomal positioning, maturation and secretion (PubMed:30771381). Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.
Ras-related protein Rab-27A, Rab-27, GTP-binding protein Ram, RAB27A, RAB27
Rabbit Recombinant Monoclonal RAB27A antibody. Suitable for IHC-P, WB, IP, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat, Transfected cell lysate - Human, Transfected cell line - Human samples.
Ras-related protein Rab-27A, Rab-27, GTP-binding protein Ram, RAB27A, RAB27
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR27120-68
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Raji (human Burkitt's lymphoma B lymphocyte, Left) / SK-MEL-28 (human malignant melanoma cell, Right) cells labelling RAB27A + RAB27B with ab313575 at 1/500 dilution (0.1 ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Negative control: Raji (PMID: 15548590).
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling RAB27A + RAB27B with ab313575 at 1/100 (5.04 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green). Confocal image showing both positive staining in 293T cells transfected with human RAB27A and RAB27B expression vectors containing a Myc tag and a Flag tag respectively. Counter stain:ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594), 1:100 5ug/ml. Anti-FLAG mouse monoclonal antibody, 1:500 2ug/ml. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SK-MEL-28 (human malignant melanoma cell) cells labelling RAB27A + RAB27B with ab313575 at 1/100 (5.04 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green). Confocal image showing cytoplasmic staining in SK-MEL-28. Negative control: Raji (PMID: 15548590). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling RAB27A + RAB27B with ab313575 at 1/5000 (0.101 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on human skeletal muscle. The section was incubated with ab313575 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue labeling RAB27A + RAB27B with ab313575 at 1/5000 (0.101 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human lung adenocarcinoma (PMID: 30480360). The section was incubated with ab313575 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
RAB27A + RAB27B was immunoprecipitated from 0.35 mg K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate with ab313575 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab313575 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 2: K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab313575 in K562 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds
All lanes: Immunoprecipitation - Anti-RAB27A + RAB27B antibody [EPR27120-68] (AB313575) at 1/30 dilution
All lanes: K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366) at 1/5000 dilution
Exposure time: 10s
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling RAB27A + RAB27B with ab313575 at 1/5000 (0.101 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human colon (PMID: 26070933). The section was incubated with ab313575 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody recognizes both RAB27A and RAB27B.
In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution.
Exposure time: 3 seconds
All lanes: Western blot - Anti-RAB27A + RAB27B antibody [EPR27120-68] (AB313575) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containi a His-tag whole cell lysate at 20 µg
Lane 2: 293T cells transfected with a human RAB27A expression vector containi a His-tag whole cell lysate at 20 µg
Lane 3: 293T cells transfected with a human RAB27B expression vector containi a Flag-tag whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/100000 dilution
Observed band size: 27 kDa
Exposure time: 3s
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: Raji (PMID:15548590)
In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.
Exposure time: 15 seconds
All lanes: Western blot - Anti-RAB27A + RAB27B antibody [EPR27120-68] (AB313575) at 1/1000 dilution
Lane 1: K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg
Lane 2: SK-MEL-2 (human skin malignant melanoma cell) whole cell lysate at 20 µg
Lane 3: Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/100000 dilution
Observed band size: 27 kDa
Exposure time: 15s
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: skeletal muscle.
In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.
Exposure time: 180 seconds
All lanes: Western blot - Anti-RAB27A + RAB27B antibody [EPR27120-68] (AB313575) at 1/1000 dilution
Lane 1: Rat stomach tissue lysate at 20 µg
Lane 2: Rat skeletal muscle tissue lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/100000 dilution
Observed band size: 27 kDa
Exposure time: 180s
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: skeletal muscle.
In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.
Exposure time: 180 seconds
All lanes: Western blot - Anti-RAB27A + RAB27B antibody [EPR27120-68] (AB313575) at 1/1000 dilution
Lane 1: Mouse stomach tissue lysate at 20 µg
Lane 2: Mouse skeletal muscle tissue lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/100000 dilution
Observed band size: 27 kDa
Exposure time: 180s
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: skeletal muscle
In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.
Exposure time: 26 seconds
All lanes: Western blot - Anti-RAB27A + RAB27B antibody [EPR27120-68] (AB313575) at 1/1000 dilution
Lane 1: Human stomach tissue lysate at 20 µg
Lane 2: Human skeletal muscle tissue lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/100000 dilution
Observed band size: 27 kDa
Exposure time: 26s
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com