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Rabbit Recombinant Monoclonal RAB27A antibody. Suitable for IHC-P, WB, IP, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat, Transfected cell lysate - Human, Transfected cell line - Human samples.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBIPICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Not recommended
Tested
Not recommended
Expected
Expected
Rat
Not recommended
Tested
Expected
Expected
Expected
Transfected cell line - Human
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Transfected cell lysate - Human
Not recommended
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

1/5000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse, Rat, Transfected cell lysate - Human, Transfected cell line - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Species

Transfected cell lysate - Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Transfected cell line - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/30

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Mouse, Transfected cell lysate - Human, Transfected cell line - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100

Notes

-

Species

Transfected cell line - Human

Dilution info

1/100

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Transfected cell lysate - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Transfected cell lysate - Human, Transfected cell line - Human

Dilution info

-

Notes

-

Target data

Function

Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate homeostasis of late endocytic pathway, including endosomal positioning, maturation and secretion (PubMed:30771381). Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.

Alternative names

Recommended products

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Rabbit Recombinant Monoclonal RAB27A antibody. Suitable for IHC-P, WB, IP, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat, Transfected cell lysate - Human, Transfected cell line - Human samples.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR27120-68

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

12 product images

  • Flow Cytometry (Intracellular) - Anti-RAB27A + RAB27B antibody [EPR27120-68] (ab313575), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-RAB27A + RAB27B antibody [EPR27120-68] (ab313575)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Raji (human Burkitt's lymphoma B lymphocyte, Left) / SK-MEL-28 (human malignant melanoma cell, Right) cells labelling RAB27A + RAB27B with ab313575 at 1/500 dilution (0.1 ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Negative control: Raji (PMID: 15548590).

  • Immunocytochemistry/ Immunofluorescence - Anti-RAB27A + RAB27B antibody [EPR27120-68] (ab313575), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-RAB27A + RAB27B antibody [EPR27120-68] (ab313575)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling RAB27A + RAB27B with ab313575 at 1/100 (5.04 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green). Confocal image showing both positive staining in 293T cells transfected with human RAB27A and RAB27B expression vectors containing a Myc tag and a Flag tag respectively. Counter stain:ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594), 1:100 5ug/ml. Anti-FLAG mouse monoclonal antibody, 1:500 2ug/ml. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). The Nuclear counterstain was DAPI (Blue).
    Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-RAB27A + RAB27B antibody [EPR27120-68] (ab313575), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-RAB27A + RAB27B antibody [EPR27120-68] (ab313575)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SK-MEL-28 (human malignant melanoma cell) cells labelling RAB27A + RAB27B with ab313575 at 1/100 (5.04 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green). Confocal image showing cytoplasmic staining in SK-MEL-28. Negative control: Raji (PMID: 15548590). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
    Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB27A + RAB27B antibody [EPR27120-68] (ab313575), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB27A + RAB27B antibody [EPR27120-68] (ab313575)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling RAB27A + RAB27B with ab313575 at 1/5000 (0.101 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on human skeletal muscle. The section was incubated with ab313575 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB27A + RAB27B antibody [EPR27120-68] (ab313575), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB27A + RAB27B antibody [EPR27120-68] (ab313575)

    Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue labeling RAB27A + RAB27B with ab313575 at 1/5000 (0.101 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human lung adenocarcinoma (PMID: 30480360). The section was incubated with ab313575 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunoprecipitation - Anti-RAB27A + RAB27B antibody [EPR27120-68] (ab313575), expandable thumbnail

    Immunoprecipitation - Anti-RAB27A + RAB27B antibody [EPR27120-68] (ab313575)

    RAB27A + RAB27B was immunoprecipitated from 0.35 mg K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate with ab313575 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab313575 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
    Lane 1: K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
    Lane 2: K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab313575 in K562 whole cell lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 10 seconds

    All lanes: Immunoprecipitation - Anti-RAB27A + RAB27B antibody [EPR27120-68] (AB313575) at 1/30 dilution

    All lanes: K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366) at 1/5000 dilution

    Exposure time: 10s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB27A + RAB27B antibody [EPR27120-68] (ab313575), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB27A + RAB27B antibody [EPR27120-68] (ab313575)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling RAB27A + RAB27B with ab313575 at 1/5000 (0.101 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human colon (PMID: 26070933). The section was incubated with ab313575 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Western blot - Anti-RAB27A + RAB27B antibody [EPR27120-68] (ab313575), expandable thumbnail

    Western blot - Anti-RAB27A + RAB27B antibody [EPR27120-68] (ab313575)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    This antibody recognizes both RAB27A and RAB27B.

    In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution.
    Exposure time: 3 seconds

    All lanes: Western blot - Anti-RAB27A + RAB27B antibody [EPR27120-68] (AB313575) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containi a His-tag whole cell lysate at 20 µg

    Lane 2: 293T cells transfected with a human RAB27A expression vector containi a His-tag whole cell lysate at 20 µg

    Lane 3: 293T cells transfected with a human RAB27B expression vector containi a Flag-tag whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/100000 dilution

    Observed band size: 27 kDa

    Exposure time: 3s

  • Western blot - Anti-RAB27A + RAB27B antibody [EPR27120-68] (ab313575), expandable thumbnail

    Western blot - Anti-RAB27A + RAB27B antibody [EPR27120-68] (ab313575)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Negative control: Raji (PMID:15548590)

    In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.
    Exposure time: 15 seconds

    All lanes: Western blot - Anti-RAB27A + RAB27B antibody [EPR27120-68] (AB313575) at 1/1000 dilution

    Lane 1: K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

    Lane 2: SK-MEL-2 (human skin malignant melanoma cell) whole cell lysate at 20 µg

    Lane 3: Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/100000 dilution

    Observed band size: 27 kDa

    Exposure time: 15s

  • Western blot - Anti-RAB27A + RAB27B antibody [EPR27120-68] (ab313575), expandable thumbnail

    Western blot - Anti-RAB27A + RAB27B antibody [EPR27120-68] (ab313575)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Negative control: skeletal muscle.

    In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.
    Exposure time: 180 seconds

    All lanes: Western blot - Anti-RAB27A + RAB27B antibody [EPR27120-68] (AB313575) at 1/1000 dilution

    Lane 1: Rat stomach tissue lysate at 20 µg

    Lane 2: Rat skeletal muscle tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/100000 dilution

    Observed band size: 27 kDa

    Exposure time: 180s

  • Western blot - Anti-RAB27A + RAB27B antibody [EPR27120-68] (ab313575), expandable thumbnail

    Western blot - Anti-RAB27A + RAB27B antibody [EPR27120-68] (ab313575)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Negative control: skeletal muscle.

    In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.
    Exposure time: 180 seconds

    All lanes: Western blot - Anti-RAB27A + RAB27B antibody [EPR27120-68] (AB313575) at 1/1000 dilution

    Lane 1: Mouse stomach tissue lysate at 20 µg

    Lane 2: Mouse skeletal muscle tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/100000 dilution

    Observed band size: 27 kDa

    Exposure time: 180s

  • Western blot - Anti-RAB27A + RAB27B antibody [EPR27120-68] (ab313575), expandable thumbnail

    Western blot - Anti-RAB27A + RAB27B antibody [EPR27120-68] (ab313575)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Negative control: skeletal muscle

    In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.
    Exposure time: 26 seconds

    All lanes: Western blot - Anti-RAB27A + RAB27B antibody [EPR27120-68] (AB313575) at 1/1000 dilution

    Lane 1: Human stomach tissue lysate at 20 µg

    Lane 2: Human skeletal muscle tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/100000 dilution

    Observed band size: 27 kDa

    Exposure time: 26s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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