Anti-RAB27A + RAB27B antibody [EPR27120-68] - BSA and Azide free (ab313576)

Rabbit Recombinant Monoclonal RAB27A antibody. Carrier free. Suitable for IHC-P, WB, IP, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat, Transfected cell lysate - Human, Transfected cell line - Human samples.

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Images

Western blot - Anti-RAB27A + RAB27B antibody [EPR27120-68] - BSA and Azide free (AB313576), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	WB	IP	ICC/IF	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Not recommended	Tested	Not recommended	Expected	Expected
Rat	Not recommended	Tested	Expected	Expected	Expected
Transfected cell line - Human	Not recommended	Tested	Not recommended	Not recommended	Not recommended
Transfected cell lysate - Human	Not recommended	Tested	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Transfected cell lysate - Human, Transfected cell line - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse, Rat, Transfected cell lysate - Human, Transfected cell line - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Transfected cell lysate - Human, Transfected cell line - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Human, Transfected cell line - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Human, Transfected cell line - Human	Dilution info -	Notes -

Associated Products

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1 product for Alternative Version

Target data

See full target information RAB27A

Function

Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate homeostasis of late endocytic pathway, including endosomal positioning, maturation and secretion (PubMed:30771381). Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.

Additional Targets

RAB27B

Alternative names

RAB27, RAB27A, Ras-related protein Rab-27A, Rab-27, GTP-binding protein Ram

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR27120-68
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

RAB27A and RAB27B are members of the Rab protein family which are small GTPases involved in membrane trafficking. These proteins also known as GTP-binding proteins are critical for vesicle transport and membrane fusion processes. RAB27A has a molecular mass of approximately 25 kDa and RAB27B has a molecular mass around 24.5 kDa. Both proteins express widely in various cell types yet show higher expression in secretory cells particularly in the brain pancreas and melanocytes. RAB27A and RAB27B localize to the membrane of secretory granules aiding in their mobilization.

Biological function summary

The proteins RAB27A and RAB27B facilitate the movement of secretory vesicles toward the cell membrane for exocytosis. RAB27A is a component of protein complexes known as exocyst complexes which are involved in tethering vesicles to the plasma membrane. The proteins recruit effector molecules such as Myosin Va and Slp2-a to the vesicle surface enhancing their docking and fusion with the cell membrane. This process is essential for regulated secretion of hormones neurotransmitters and other important molecules.

Pathways

RAB27A and RAB27B play important roles in the vesicular transport systems that govern secretion pathways including the exocytosis and endocytosis pathways. During exocytosis RAB27A works closely with SNARE proteins to stabilize vesicle fusion. It also interacts with other Rab proteins like RAB3D contributing to efficient vesicle trafficking. In addition RAB27B has a role in synaptic vesicle pathways which are key to neurotransmitter release in neural communication.

Associated diseases and disorders

Mutations in RAB27A lead to Griscelli syndrome type 2 characterized by immune system dysfunction and pigment dilution. This syndrome occurs due to defective vesicle transport in melanocytes and immune cells. RAB27A is linked with LYST a protein involved in lysosomal trafficking which causes Chediak-Higashi syndrome when mutated. Moreover altered RAB27B expression associates with colorectal cancer progression emphasizing its importance in cellular secretion dysfunctions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

15 product images

Western blot - Anti-RAB27A + RAB27B antibody [EPR27120-68] - BSA and Azide free (ab313576)
This data was developed using Anti-RAB27A + RAB27B antibody [EPR27120-68] ab313575, the same antibody clone in a different buffer formulation.
Anti-RAB27A + RAB27B antibody [EPR27120-68] ab313575 was shown to react with Rab27A in wild-type U-87 MG cells in Western blot with loss of signal observed in a Rab27A siRNA knockdown cell line. Cell lysates from wild-type U-87 MG transfected with either scrambled siRNA or Rab27A siRNA were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with Anti-RAB27A + RAB27B antibody [EPR27120-68] ab313575 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
All lanes: Western blot - Anti-RAB27A + RAB27B antibody [EPR27120-68] (Anti-RAB27A + RAB27B antibody [EPR27120-68] ab313575) at 1/1000 dilution
Lane 1: Wild-type U-87 MG transfected with scrambled siRNA control lysate at 20 µg
Lane 2: U-87 MG transfected with siRNA specifically targeting Rab27A cell lysate at 20 µg
Immunoprecipitation - Anti-RAB27A + RAB27B antibody [EPR27120-68] - BSA and Azide free (ab313576)
This data was developed using Anti-RAB27A + RAB27B antibody [EPR27120-68] ab313575, the same antibody clone in a different buffer formulation.
Immunoprecipitation of Rab27A in U-87 MG cells. Lysates were prepared and immunoprecipitation was performed using 2 ?g of Anti-RAB27A + RAB27B antibody [EPR27120-68] ab313575 pre-coupled to Protein A beads. Samples were then washed and processed for western blot.

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
All lanes: Immunoprecipitation - Anti-RAB27A + RAB27B antibody [EPR27120-68] (Anti-RAB27A + RAB27B antibody [EPR27120-68] ab313575) at 2 µg
Lanes 1 and 3: U-87 MG cells
Lane 2: Unbound fraction: Rabbit monoclonal IgG instead of Anti-RAB27A + RAB27B antibody [EPR27120-68] ab313575 in U-87 MG cells
Immunocytochemistry/ Immunofluorescence - Anti-RAB27A + RAB27B antibody [EPR27120-68] - BSA and Azide free (ab313576)
This data was developed using Anti-RAB27A + RAB27B antibody [EPR27120-68] ab313575, the same antibody clone in a different buffer formulation.
Anti-RAB27A + RAB27B antibody [EPR27120-68] ab313575 was shown to react with Rab27A in wild-type U-87 MG cells in immunocytochemistry with loss of signal observed in a Rab27A siRNA knockdown cell line. Wild-type and siRNA knockdown cells were mixed and pelleted at a 1:1 ratio on coverslips. The cells were fixed with 4% paraformaldehyde (15 min) then permeabilized with 0.1% Triton X-100 (10min) and then blocked with 1x PBS, 0.01% Triton X-100, 5% BSA, 5% NGS. The cells were then incubated with Anti-RAB27A + RAB27B antibody [EPR27120-68] ab313575 at 1/500 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat anti-rabbit secondary antibody to (Alexa Fluor® 555) at 0.5 ?g/ml. Acquisition of the green (wild-type), red (antibody staining) and far-red (siRNA knockdown) channels was performed. Representative grayscale images of the red channel are shown. Wild-type and siRNA knockdown cells are outlined with yellow and magenta dashed line, respectively. Schematic representation of the mosaic strategy used is shown on the bottom-right panel. Image was acquired with a Zeiss(LSM-880).

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
Flow Cytometry (Intracellular) - Anti-RAB27A + RAB27B antibody [EPR27120-68] - BSA and Azide free (ab313576)
This data was developed using Anti-RAB27A + RAB27B antibody [EPR27120-68] ab313575, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Raji (human Burkitt's lymphoma B lymphocyte, Left) / SK-MEL-28 (human malignant melanoma cell, Right) cells labelling RAB27A + RAB27B with Anti-RAB27A + RAB27B antibody [EPR27120-68] ab313575 at 1/500 dilution (0.1 ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody. Negative control: Raji (PMID: 15548590).
Immunocytochemistry/ Immunofluorescence - Anti-RAB27A + RAB27B antibody [EPR27120-68] - BSA and Azide free (ab313576)
This data was developed using Anti-RAB27A + RAB27B antibody [EPR27120-68] ab313575, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling RAB27A + RAB27B with Anti-RAB27A + RAB27B antibody [EPR27120-68] ab313575 at 1/100 (5.04 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green). Confocal image showing both positive staining in 293T cells transfected with human RAB27A and RAB27B expression vectors containing a Myc tag and a Flag tag respectively. Counter stain:Alexa Fluor® 594 Anti-Myc tag antibody [9E10] ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594), 1:100 5ug/ml. Anti-FLAG mouse monoclonal antibody, 1:500 2ug/ml. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.
Immunocytochemistry/ Immunofluorescence - Anti-RAB27A + RAB27B antibody [EPR27120-68] - BSA and Azide free (ab313576)
This data was developed using Anti-RAB27A + RAB27B antibody [EPR27120-68] ab313575, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SK-MEL-28 (human malignant melanoma cell) cells labelling RAB27A + RAB27B with Anti-RAB27A + RAB27B antibody [EPR27120-68] ab313575 at 1/100 (5.04 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green). Confocal image showing cytoplasmic staining in SK-MEL-28. Negative control: Raji (PMID: 15548590). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB27A + RAB27B antibody [EPR27120-68] - BSA and Azide free (ab313576)
This data was developed using Anti-RAB27A + RAB27B antibody [EPR27120-68] ab313575, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling RAB27A + RAB27B with Anti-RAB27A + RAB27B antibody [EPR27120-68] ab313575 at 1/5000 (0.101 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on human skeletal muscle. The section was incubated with Anti-RAB27A + RAB27B antibody [EPR27120-68] ab313575 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB27A + RAB27B antibody [EPR27120-68] - BSA and Azide free (ab313576)
This data was developed using Anti-RAB27A + RAB27B antibody [EPR27120-68] ab313575, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue labeling RAB27A + RAB27B with Anti-RAB27A + RAB27B antibody [EPR27120-68] ab313575 at 1/5000 (0.101 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human lung adenocarcinoma (PMID: 30480360). The section was incubated with Anti-RAB27A + RAB27B antibody [EPR27120-68] ab313575 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunoprecipitation - Anti-RAB27A + RAB27B antibody [EPR27120-68] - BSA and Azide free (ab313576)
This data was developed using Anti-RAB27A + RAB27B antibody [EPR27120-68] ab313575, the same antibody clone in a different buffer formulation.
RAB27A + RAB27B was immunoprecipitated from 0.35 mg K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate with Anti-RAB27A + RAB27B antibody [EPR27120-68] ab313575 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-RAB27A + RAB27B antibody [EPR27120-68] ab313575 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 2: K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-RAB27A + RAB27B antibody [EPR27120-68] ab313575 in K562 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds
All lanes: Immunoprecipitation - Anti-RAB27A + RAB27B antibody [EPR27120-68] (Anti-RAB27A + RAB27B antibody [EPR27120-68] ab313575) at 1/30 dilution
All lanes: K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 10s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB27A + RAB27B antibody [EPR27120-68] - BSA and Azide free (ab313576)
This data was developed using Anti-RAB27A + RAB27B antibody [EPR27120-68] ab313575, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling RAB27A + RAB27B with Anti-RAB27A + RAB27B antibody [EPR27120-68] ab313575 at 1/5000 (0.101 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human colon (PMID: 26070933). The section was incubated with Anti-RAB27A + RAB27B antibody [EPR27120-68] ab313575 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Western blot - Anti-RAB27A + RAB27B antibody [EPR27120-68] - BSA and Azide free (ab313576)
This data was developed using Anti-RAB27A + RAB27B antibody [EPR27120-68] ab313575, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody recognizes both RAB27A and RAB27B.

In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
Exposure time: 3 seconds
All lanes: Western blot - Anti-RAB27A + RAB27B antibody [EPR27120-68] (Anti-RAB27A + RAB27B antibody [EPR27120-68] ab313575) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containi a His-tag whole cell lysate at 20 µg
Lane 2: 293T cells transfected with a human RAB27A expression vector containi a His-tag whole cell lysate at 20 µg
Lane 3: 293T cells transfected with a human RAB27B expression vector containi a Flag-tag whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 27 kDa
Exposure time: 3s
Western blot - Anti-RAB27A + RAB27B antibody [EPR27120-68] - BSA and Azide free (ab313576)
This data was developed using Anti-RAB27A + RAB27B antibody [EPR27120-68] ab313575, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: Raji (PMID:15548590)

In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 15 seconds
All lanes: Western blot - Anti-RAB27A + RAB27B antibody [EPR27120-68] (Anti-RAB27A + RAB27B antibody [EPR27120-68] ab313575) at 1/1000 dilution
Lane 1: K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg
Lane 2: SK-MEL-2 (human skin malignant melanoma cell) whole cell lysate at 20 µg
Lane 3: Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 27 kDa
Exposure time: 15s
Western blot - Anti-RAB27A + RAB27B antibody [EPR27120-68] - BSA and Azide free (ab313576)
This data was developed using Anti-RAB27A + RAB27B antibody [EPR27120-68] ab313575, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: skeletal muscle.

In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 180 seconds
All lanes: Western blot - Anti-RAB27A + RAB27B antibody [EPR27120-68] (Anti-RAB27A + RAB27B antibody [EPR27120-68] ab313575) at 1/1000 dilution
Lane 1: Rat stomach tissue lysate at 20 µg
Lane 2: Rat skeletal muscle tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 27 kDa
Exposure time: 180s
Western blot - Anti-RAB27A + RAB27B antibody [EPR27120-68] - BSA and Azide free (ab313576)
This data was developed using Anti-RAB27A + RAB27B antibody [EPR27120-68] ab313575, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: skeletal muscle.

In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 180 seconds
All lanes: Western blot - Anti-RAB27A + RAB27B antibody [EPR27120-68] (Anti-RAB27A + RAB27B antibody [EPR27120-68] ab313575) at 1/1000 dilution
Lane 1: Mouse stomach tissue lysate at 20 µg
Lane 2: Mouse skeletal muscle tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 27 kDa
Exposure time: 180s
Western blot - Anti-RAB27A + RAB27B antibody [EPR27120-68] - BSA and Azide free (ab313576)
This data was developed using Anti-RAB27A + RAB27B antibody [EPR27120-68] ab313575, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: skeletal muscle

In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 26 seconds
All lanes: Western blot - Anti-RAB27A + RAB27B antibody [EPR27120-68] (Anti-RAB27A + RAB27B antibody [EPR27120-68] ab313575) at 1/1000 dilution
Lane 1: Human stomach tissue lysate at 20 µg
Lane 2: Human skeletal muscle tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 27 kDa
Exposure time: 26s